Histological Analysis of Somatic Embryogenesis in Itoh Peony

Boltenkov, Eugeny V.; Kuritskaya, Elena Vladimirovna; Vrzhosek, E. V.

doi:10.18520/cs/v111/i2/395-398

Cited by 3 publications

(2 citation statements)

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“…Our results show that a combination of cytokinin (BA or TDZ) and auxin (2,4-D) was effective in inducing somatic embryos in P. ostii 'Fengdan'. A combination of cytokinin (BA) and auxin (IBA) could also enhance somatic embryo induction from buds of Iton peony [23]. Numerous studies on the somatic embryogenesis in a wide range of species show that auxins have a critical role in the somatic embryo induction and cytokinins are also frequently involved [21].…”

Section: Discussionmentioning

confidence: 99%

“…Abscisic acid (ABA) or a combination of BA and NAA could stimulate somatic embryogenesis from cotyledon explant of peony [18,32]. Phenylacetic acid (PAA) enhances somatic embryo induction from anther explant of peony [32], and a combination of BA and IBA induces somatic embryogenesis from shoot explant of Itoh peony [23]. The type of auxin and cytokinin and their optimum concentration for embryo induction have been proven to be different for different explants in many other plants [33,34].…”

Section: Discussionmentioning

confidence: 99%

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Enhanced Somatic Embryo Induction of a Tree Peony, Paeonia ostii ‘Fengdan’, by a Combination of 6-benzylaminopurine (BA) and 1-naphthylacetic Acid (NAA)

Ren

Jeong

2019

Plants

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Somatic embryogenesis is a preferred method for vegetative propagation due to its high propagation efficiency. In this study, zygotic embryos, cotyledons, and hypocotyls of Paeonia ostii ‘Fengdan’ were used as the explant to induce somatic embryogenesis. The results showed that a combination of 0.5 mg·L−1 thidiazuron (TDZ) and 0.5 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D) was effective in inducing somatic embryos from the zygotic embryo and cotyledon explants. Hypocotyls only formed somatic embryos on Murashige and Skoog (MS) medium supplemented with both 0.5 mg·L−1 TDZ and 0.5 mg·L−1 1-naphthylacetic acid (NAA). Moreover, the compact callus was effectively produced from zygotic embryo, cotyledon, and hypocotyl explants in medium supplemented with a combination of 3.0 mg·L−1 6-benzylaminopurine (BA) and 1.0 mg·L−1 NAA, and then converted into somatic embryos in the same medium, and the ratio of the explants with embryo induction and number of embryos induced per explant were much higher than those induced by 0.5 mg·L−1 TDZ and either 0.5 mg·L−1 2,4-D or 0.5 mg·L−1 NAA. The MS medium was better than the woody plant medium (WPM) for inducing somatic embryos from zygotic embryo and hypocotyl explants, whereas the WPM was better than the MS medium for somatic embryogenesis induction from cotyledon explants. All of the somatic embryos developed well into mature embryos on their respective media supplemented with both 3.0 mg·L−1 BA and 1.0 mg·L−1 NAA. Overall, the protocols for indirect somatic embryogenesis from zygotic embryo, cotyledon, and hypocotyl of P. ostii ‘Fengdan’ were successfully established, which can greatly facilitate their propagation and breeding processes.

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Section: Discussionmentioning

confidence: 99%