Histologic Studies on Percutaneous Penetration with Special Reference to the Effect of Vehicles12

MacKee, George M.; Sulzberger, Marion B.; Herrmann, F.; Baer, Rudolf L.

doi:10.1038/jid.1945.6

Cited by 113 publications

(10 citation statements)

References 6 publications

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“…Hair follicles were discussed as an important penetration pathway due to a higher penetration rate into skin with a high density of follicles, compared with a skin site with or without a low density of follicles [44][45][46][47][48] . Penetration into the follicles seems to be the faster process compared with that via the SC [45,49] . Recently, the follicular penetration has been determined for organic dyes [41,42] , DNA [50] and topically applied nanoparticles [40] .…”

Section: Discussionmentioning

confidence: 99%

Penetration of Pollen Proteins into the Skin

Jacobi

Engel²,

Patzelt³

et al. 2007

Skin Pharmacol Physiol

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Atopic dermatitis is a chronic remittent skin disease. In the extrinsic form of atopic dermatitis, type IgE-mediated reactions play an important pathophysiological role. The aim of the present study was to examine whether type I allergens can penetrate into the skin. Therefore, pollen proteins were labeled with fluorescein isothiocyanate (FITC), and their penetration profile was studied qualitatively. Solutions of FITC-labeled pollen proteins were applied in vitro on porcine skin and in vivo on human skin. In vitro, the FITC-labeled proteins were observed within the complete stratum corneum (SC) and inside the hair follicles even 15 min after application. They were also distributed inside the dermis around the hair follicles. In vivo, a similar pattern of distribution within the SC and the hair follicles was observed. These results indicate penetration via the SC lipid layers and a faster penetration via the hair follicles. The FITC-labeled proteins entered the dermis via the follicular pathway. Therefore, the follicular penetration should be considered in the development of skin protection strategies. To evaluate such strategies, the developed method can be used, and further studies in atopic dermatitis patients are necessary to determine whether the penetration of type I allergens is increased.

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Section: Discussionmentioning

confidence: 99%

Penetration of Pollen Proteins into the Skin

Jacobi

Engel²,

Patzelt³

et al. 2007

Skin Pharmacol Physiol

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“…Die Schwcissdrüsenausführungsgänge spielen für den Penetra tionsweg des Antigens ebenfalls kaum eine Rolle, wie wir aus den U ntersuchungen mittels Analyse von Farbstoifresorption [7], aber [3], dem es gelang, Meerschweinchen perkutan gegen Pferde serum mit Hilfe einer serum getränkten Kompresse zu sensibilisieren und anschliessend durch intravenöse Reinjektion einen anaphylakti schen Schock auszulösen -, ferner die Untersuchungen von Kirnura [6], der durch Einreiben einer Eiklarvaseline Kaninchen mit Erfolg sensibilisierte u.a.m .…”

Section: Von W Gronemeyer Und M D Ebelicunclassified

Der sogenannte «Reibtest», seine Anwendung und klinische Bedeutung

Gronemeyer¹,

Debelić²

1967

Dermatology

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“…This resistance is present to many substances coming from within, as well as to those coming from without. It has been shown that the points of weakest resistance are located in the follicular walls [62]. The actual cause or mechanism of this " physiologic weakness" is not clear and in part still controversial.…”

Section: ( I Ll ) Functions O F Stratum Disjunction and Conjunctummentioning

confidence: 99%