Histologic Evidence of New Collagen Formulation Using Platelet Rich Plasma in Skin Rejuvenation: A Prospective Controlled Clinical Study

Abstract

BackgroundPlatelet-rich plasma (PRP) is an autologous concentration of human platelets contained in a small volume of plasma and has recently been shown to accelerate rejuvenate aging skin by various growth factors and cell adhesion molecules.ObjectiveThis study was conducted to evaluate the efficacy and safety of intradermal injection of PRP in the human facial rejuvenation.MethodsThis study was a prospective, single-center, single-dose, open-label, non-randomized controlled clinical study. PRP injected to the upper site of this right infra-auricular area and all face. Saline was injected to the left infra-auricular area. Histopathological examinations were performed before PRP treatment, 28 days after the PRP, and saline (control) treatments.ResultsTwenty women ranging in age from 40 to 49 years (mean age, 43.65±2.43 years) were enrolled in the study. The mean optical densities (MODs) of collagen in the pre-treatment, control, and PRP-treated area were measured. They were 539±93.2, 787±134.15, 1,019±178, respectively. In the MOD of PRP, 89.05 percent improvement was found when MOD of PRP was compared with MOD of pre-treatment. The mean MOD of collagen fibers was clearly highest on the PRP side (p<0.001). The PRP-to-saline improvement ratio (89.05% to 46.01%) was 1.93:1. No serious side effects were detected.ConclusionPRP increases dermal collagen levels not only by growth factors, but also by skin needling (the mesotherapy technique 'point by point'). PRP application could be considered as an effective (even a single application) and safety procedure for facial skin rejuvenation.

Differential effect of platelet-rich plasma fractions on &beta;1-integrin signaling, collagen biosynthesis, and prolidase activity in human skin fibroblasts

“…Numerous reports suggest the use of PRP in delayed bone healing and nonunions, chondropathy, osteoarthritis, tendinopathy, acute and chronic soft-tissue injuries, enhancement of healing after ligament reconstruction, muscle strains, and in skin rejuvenation 2–4,20. The specific role of collagen in bones, tendons, ligaments and skin repair is well established 21,22.…”

Re: Histologic Evidence of New Collagen Formulation Using Platelet Rich Plasma in Skin Rejuvenation: A Prospective Controlled Clinical Study: Authors' Reply

“…Secondly, we are completely agreed that the efficiency of treatment depends on the platelet concentration in PRP. A sterfile Conformité Européenne (CE) marked RegenLab Ⓡ kit (Regen Lab., Le Mont-sur-Lausanne, Switzerland) was used for preparation of PRP 1 . We are sorry that we did not perform a platelet count to our plasma samples.…”

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

“…PRF has been widely used in clinical practice to improve the efficacy of cell-assisted lipotransfer (CAL) and wound repair by ASCs, due to its advantages in preventing immunologic rejection, simple production, and few complications. Moreover, plastic surgeons have adopted PRP, a predecessor of PRF, as a rejuvenator to treat aging skin by intradermal injection [8, 34, 35]. …”