Histochemical studies of the ovarian nurse tissues and oocytes of the milkweed bug, oncopeltus fasciatus (dallas) I. Cytology, nucleic acids, and carbohydrates

Cytochemical Studies on the Origin and Composition of Yolk in <i>Chrysocoris stollii</i> (Hemiptera)

“…Besides, no PAS-positive material has either been seen in or coming out of the folli cular epithelium in the present case. Such yolk spheres of Chrysocoris, however, resemble broadly speaking, in their histochemical nature, the yolk spheres of cock roach (Nath et al 1959 d), Culex (Nath et al 1958 a), Chrotogonus, Gryllodes and Labidura (Nath et al 1959a,b), Laccotrephes (Nath et al 1959d), Oncopeltus (Bonhag 1955), Locusta (Gupta 1968) and Oxya (Verma and Das 1974) inasmuch as they are protein-carbohydrate in nature. Nath et al (1958a,c;1959a,b,d) and Aggarwal (1962) have studied the dis tribution and chemical composition of mitochondria during oogenesis of several insects.…”

Histochemical analysis of origin and composition of compound yolks of Chrotogonus trachypterus (Orthoptera).

“…This shows the presence of glycogen chemically bonded with proteins (muco-or glycoproteins), which is resistant to these enzymes. Such muco-or glycoprotein nature of the yolk bodies has also been derived in the oocytes of Oncopeltus fasciatus (Bonhag 1955), Anisolabis mari tima (Bonhag 1956) and Panorpa communis (Ramamurty 1968). Ramamurty has postulated the synthesis of glycogen taking place in the oocyte itself.…”

“…In the support of this contention it may be pertinent to mention that the young oocytes of insects, do reportedly consist glycogen synthetase (Engles and Bier 1967), which is necessary for glycogen synthe sis. The yolks developing as granules (glycogen positive) in the central ooplasm have been recorded in Anisolabis by Bonhag (1956) and in Calosoma by Mohanty (1977). In the case of Chrotogonus also the CY2 bodies appear as glycogen granules (malt diastase and salivary amylase resistant) possibly conjugated with proteins (muco-or glycoproteins) in the central ooplasm of oocyte of stage 8.…”

A Cytochemical Study of Ribonucleic Acid Distribution in the Spermatogenic Cells of <i>Drosophila virilis</i>

“…Such a decrease in RNA synthesis could possibly account for some of the observed reduction in cyto plasmic basophilia in the advanced growth primary spermatocytes. According to Bohnag (1955), the decrease in cytoplasmic basophilia in the oocytes of the milk weed bug Oncopeltus fasciatus resulted from the volumetric increase that occurred during growth of the oocytes rather than a reduction in total RNA content. Since the primary spermatocytes of D. virilis undergo an approximate 300-fold increase in volume (Ito 1960), some of the decrease in cytoplasmic basophilia in the advanced growth primary spermatocytes may simply be a dilution resulting from the tre mendous increase in volume of the primary spermatocytes.…”