Cytochemical Studies on the Origin and Composition of Yolk in <i>Chrysocoris stollii</i> (Hemiptera)

Oogenesis in <i>Cybister guaerini</i> Anbe (Coleoptera): Studies on the Origin and Composition of Yolk

“…While Verma and Basiston (1974) in Chrysocoris find direct involvement of nucleolar extrusions in the formation of yolk, the present authors do not agree with this view because the nucleolar extrusions in Cybister guaerini dissolve out in the ooplasm long before the appearance of yolk. However, being rich in RNA and pro tein the nucleolar extrusions can easily provide the raw materials for the production of 'finished' yolk.…”

“…However, being rich in RNA and pro tein the nucleolar extrusions can easily provide the raw materials for the production of 'finished' yolk. Such conclusion has also been drawn by Verma et al (1974) in the oogenesis of a spider Araneus nauticus. Basiston et al (1973), Verma and Das (1974) and Verma and Basiston (1974) have detected three categories of lipid bodies (L1, L2 and L3) during oogenesis of different insects.…”

“…Such conclusion has also been drawn by Verma et al (1974) in the oogenesis of a spider Araneus nauticus. Basiston et al (1973), Verma and Das (1974) and Verma and Basiston (1974) have detected three categories of lipid bodies (L1, L2 and L3) during oogenesis of different insects. They conclude that L3 bodies are the fatty yolks formed by the direct transformation of Golgi bodies.…”

“…Soon after they aggregate at periphery of the oocyte, enlarge and turn into fuchsinophilic bodies but a few of them continue to remain aurantiaphilic. King (1960) in Drosophila, Verma and Basiston (1974) in Chrysocoris have also noted a comparable derivation of mitochondrial yolk. CY3 bodies of Cybister however arise de novo in any zone of the ooplasm.…”

“…The materials fixed in formol-calcium were post chromed and embedded in gelatin for frozen sections, and those in other fixatives were processed as usual for paraffin sections. Different methods (see Verma and Basiston 1974) for the detection of Golgi bodies, mitochondria, carbohydrates, proteins, lipids and nucleic acids, were adopted as detailed in Gurr (1958Gurr ( , 1962 and Pearse (1968).…”