Histochemical Recognition of Calcium Oxalate

Pizzolato, Philip

doi:10.1177/12.5.333

Cited by 122 publications

(64 citation statements)

References 15 publications

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“…(17) Briefly, sequential paraffin sections were dewaxed and rinsed with distilled water. For Pizzolato staining, 30% hydrogen peroxide (1 mL) was added to the slide with tissue sections (pH 6.0).…”

Section: Animal Proceduresmentioning

confidence: 99%

Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice: Detection by association analysis of stone-related gene expression and microstructural observation

Okada

Yasui

Fujii

et al. 2010

Journal of Bone and Mineral Research

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Mice have a strong ability to eliminate renal calcium oxalate crystals, and our previous examination indicated a susceptibility in which monocyte-macrophage interaction could participate in the phenomenon. To clarify the macrophage-related factors playing roles in the prevention of crystal formation in mouse kidneys, morphologic and expression studies based on microarray pathway analysis were performed. Eight-week-old male C57BL/6N mice were administered 80 mg/kg of glyoxylate by daily intraabdominal injection for 15 days, and the kidneys were extracted every 3 days for DNA microarray analysis. Based on the raw data of microarray analysis, pathway analyses of inflammatory response demonstrated macrophage activation through the increased expression of chemokine (C-X-C) ligand 1, fibronectin 1, and major histocompatability (MHC) class II. Association analysis of related gene expression values by quantitative reverse transcription polymerase chain reaction (RT-PCR) indicated the high association of chemokine (C-C) ligand 2, CD44, colony-stimulating factor 1, fibronectin 1, matrix gla protein, secreted phosphoprotein 1, and transforming growth factor b1 (TGF-b1) with the amount of both renal crystals and F4/80, a macrophage marker. Immunohistochemically, interstitial macrophages increased during the experimental course, and CD44 and MHC class II were upregulated around crystal-formation sites. Ultrastructural observation of renal macrophages by transmission electron microscopy indicated interstitial macrophage migration with the phagocytosis of crystals. In conclusion, increased expression of inflammation-related genes of renal tubular cells induced by crystal formation and deposition could induce monocyte-macrophage migration and phagocytosis via the interaction of CD44 with osteopontin and fibronectin. Such crystalremoving ability of macrophages through phagocytosis and digestion might become a new target for the prevention of stone formation. ß

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Section: Animal Proceduresmentioning

confidence: 99%

Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice: Detection by association analysis of stone-related gene expression and microstructural observation

Okada

Yasui

Fujii

et al. 2010

Journal of Bone and Mineral Research

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“…They were rinsed in distilled water, immersed in a 1% alizarin red solution (a special stain for calcium) for 2 min, rinsed again in distilled water and reexamined under the light microscope. [31][32][33] We then performed full-thickness sections through the optic of the explanted lenses. Some of the resultant cylindrical blocks were directly stained with 1% alizarin red.…”

Section: Analyses Performed In Our Laboratorymentioning

confidence: 99%

“…Calcium salts stain dark brown with this technique. [31][32][33] Some lenses in each group were air-dried at room temperature for 7 days, sputter-coated with aluminium and examined under a JEOL JSM 5410LV scanning electron microscope (SEM). The specimens were then further analysed by Dr DG Dunkelberger (Electron Microscopy Center of the University of South Carolina, Columbia, SC, USA) under a Hitachi 2500 Delta scanning electron microscope equipped with a Kevex X-ray detector with light element capabilities for energy-dispersive X-ray spectroscopic analyses (EDS).…”

Section: Analyses Performed In Our Laboratorymentioning

confidence: 99%

Calcification of modern foldable hydrogel intraocular lens designs

Izak

Werner

Pandey

et al. 2003

Eye

122

114

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Purpose To report and compare clinical and pathological features of hydrophilic acrylic intraocular lenses (IOLs) of three major designs, explanted from patients who had visual disturbances caused by opacification of the lens optic. Methods Eighty-seven hydrophilic acrylic IOLs (25 Hydroview TM , 54 SC60B-OUV, and 8 Aqua-Sense TM lenses) were explanted and sent to our center. Most patients became symptomatic during the second year after cataract surgery. A fine granularity was observed on the surface of the lens optic in the case of Hydroview TM . With the SC60B-OUV and Aqua-Sense TM lenses, the opacity resembled a nuclear cataract. Gross examination, light microscopy and staining with alizarin red and the von Kossa method (for calcium) were performed. Some lenses were submitted for scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). Results Light microscopic and SEM analyses revealed the presence of irregular granular deposits on the external optical surfaces of Hydroview TM lenses. With the SC60B-OUV lenses, the opacity was caused by the presence of multiple fine, granular deposits within the lens optic, distributed in a line parallel to the anterior and posterior curvatures of the optic, with a clear zone just beneath its external surfaces. The Aqua-Sense TM lenses exhibited both patterns simultaneously. The deposits in all cases stained positive with alizarin red and von Kossa method. EDS also demonstrated the presence of calcium and phosphates within the deposits. Conclusion Differences in the water content of the hydrophilic acrylic materials used in the manufacture of these three lens designs may be responsible for the different patterns of calcium precipitation. Careful clinical follow up of patients implanted with these lenses is necessary to determine if this phenomenon is rare and sporadic or may be more widespread.

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“…Sagittal sections were performed and stained using the von Kossa method for calcium (Ca). [48][49][50] One lens was analyzed under scanning electron microscope (SEM).…”

Section: Analyses Of Explanted Iolsmentioning

confidence: 99%

Post cataract-intraocular lens (IOL) surgery opacification

Trivedi

Werner

Apple

et al. 2002

Eye

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Intraocular lens (IOL) implantation has no doubt been one of the most satisfying advances of medicine. Millions of individuals with visual disability or frank blindness from cataracts had and continue to have benefit from this procedure. It has been reported by ophthalmologists that the modern cataract-intraocular lens (IOL) surgery is safe and complication-free most of the time. This makes the watchword for any cataract surgeon to be 'implantation,' 'implantation,' 'implantation.' In the mid1980s, as IOLs were evolving rapidly, the watchword of the implant surgeon was 'fixation,' 'fixation,' 'fixation.' Most techniques, lenses and surgical adjuncts now allow us to achieve the basic requirement for successful IOL implantation, namely longterm stable IOL fixation in the capsular bag.However despite this advancement some items 'slipped through cracks.' In this article, we would like to alert the reader to a new watchword, namely 'opacification,' 'opacification,' 'opacification.' Here we will be talking about the good, the bad, and the ugly. Examples of the 'good' include the recent successes now being achieved in reducing the incidence of posterior capsule opacification. Examples of the 'bad' include various proliferations of anterior capsule cells, problems caused by silicone oil adherence to IOLs and problems with piggyback IOLs. The 'ugly' include the sometimes striking and often visually disabling opacifications occurring on and within IOL optics, both on some modern foldable IOLs as well as a poly(methyl methacrylate) (PMMA) optic degradation occurring with some models a decade or more after implantation.

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Histochemical Recognition of Calcium Oxalate

Cited by 122 publications

References 15 publications

Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice: Detection by association analysis of stone-related gene expression and microstructural observation

Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice: Detection by association analysis of stone-related gene expression and microstructural observation

Calcification of modern foldable hydrogel intraocular lens designs

Post cataract-intraocular lens (IOL) surgery opacification

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