Histochemical Methods for Acid Phosphatase Using Hexazonium Pararosanilin as Coupler

Barka, Tibor; Anderson, Paul J.

doi:10.1177/10.6.741

Cited by 1,514 publications

(329 citation statements)

References 0 publications

Supporting

Mentioning

317

Contrasting

Unclassified

Order By: Relevance

“…Activities of lysozyme and acid phosphatase were determined according to the method described by Deng et al [39] and Classics Barka and Anderson [42], respectively. Complement component 3 levels was assayed as in Wang et al [43].…”

Section: Plasma Ammonia Content (Pac) and Intestinal Immune Parametermentioning

confidence: 99%

The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine

Luo

Feng

Jiang³

et al. 2014

Fish & Shellfish Immunology

155

113

View full text Add to dashboard Cite

Section: Plasma Ammonia Content (Pac) and Intestinal Immune Parametermentioning

confidence: 99%

The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine

Luo

Feng

Jiang³

et al. 2014

Fish & Shellfish Immunology

155

113

View full text Add to dashboard Cite

“…After the first 100 sections, two steps of 50 sections and a final one of 100 were made, the first 4 sections from each step being selected for staining. To determine the size and position of the ischemic area, these sections were stained using the histotopochemical acid phosphatase reaction according to Barka and Anderson (1962) as modified by Meier-Ruge et al (1987). The stereologic measurement was performed with a Leitz "A SBA" optical-electronic image analysis system.…”

Section: Chemical and Histochemical Analysismentioning

confidence: 99%

Extracellular Antioxidants and Amino Acids in the Cortex of the Rat: Monitoring by Microdialysis of Early Ischemic Changes

Landolt

Lutz²,

Langemann³

et al. 1992

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

Summary: Extracellular concentrations of ascorbic acid, glutathione, cysteine, uric acid, tyrosine, and tryptophan were monitored using intracerebral microdialysis in the left frontoparietal cortex of spontaneous hypertensive rats before, and for 3 h after, either focal ischemia [left middle cerebral artery occlusion (MCAO)] or sham oper ation. The size of the ischemic area and the position of the microdialysis probe were checked using the enzyme his totopochemical acid phosphatase reaction. The probe was always located in the cortex inside the stained area. Ascorbic acid levels rose immediately after MCAO and remained at about 12-fold for 3 h. There was a transient Microdialysis (Tossman and V ngerstedt, 1986; Benveniste and Huttemeier, 1990) provides a mini mally invasive method for continuously monitoring changes in concentrations of low-molecular-weight substances in the extracellular (EC) fluid of various organs. It has been used to study cerebral ischemia in the rat, measurements being made mainly in the striatum, one of the areas most susceptible to isch emic damage. Changes have been found in this re gion at the onset of cerebral ischemia in the EC concentrations of various parameters, e. g. , ascor bic acid (Hillered et aI. , 1988), lactate (Hillered et aI., 1989), purine metabolites (Hagberg et aI. , 1987),

show abstract

“…Alternate serial cross-sections were stained with 1% toluidine blue/1% borax or submitted to the following reactions: acid phosphatase (8), myosin ATPase activity after alkali, pH 10.3 (9), or acid preincubation, pH 4.3 (10).…”

mentioning

confidence: 99%

Regenerated rat skeletal muscle after periodic contusions

Minamoto

Bunho

Salvini

2001

Braz J Med Biol Res

View full text Add to dashboard Cite

In the present study we evaluated the morphological aspect and changes in the area and incidence of muscle fiber types of long-term regenerated rat tibialis anterior (TA) muscle previously submitted to periodic contusions. Animals received eight consecutive traumas: one trauma per week, for eight weeks, and were evaluated one (N = 8) and four (N = 9) months after the last contusion. Serial cross-sections were evaluated by toluidine blue staining, acid phosphatase and myosin ATPase reactions. The weight of injured muscles was decreased compared to the contralateral intact one (one month: 0.77 ± 0.15 vs 0.91 ± 0.09 g, P = 0.03; four months: 0.79 ± 0.14 vs 1.02 ± 0.07 g, P = 0.0007, respectively) and showed abundant presence of split fibers and fibers with centralized nuclei, mainly in the deep portion. Damaged muscles presented a higher incidence of undifferentiated fibers when compared to the intact one (one month: 3.4 ± 2.1 vs 0.5 ± 0.3%, P = 0.006; four months: 2.3 ± 1.6 vs 0.3 ± 0.3%, P = 0.007, respectively). Injured TA evaluated one month later showed a decreased area of muscle fibers when compared to the intact one (P = 0.003). Thus, we conclude that: a) muscle fibers were damaged mainly in the deep portion, probably because they were compressed against the tibia; b) periodic contusions in the TA muscle did not change the percentage of type I and II muscle fibers; c) periodically injured TA muscles took four months to reach a muscle fiber area similar to that of the intact muscle.

show abstract

Histochemical Methods for Acid Phosphatase Using Hexazonium Pararosanilin as Coupler

Cited by 1,514 publications

References 0 publications

The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine

The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine

Extracellular Antioxidants and Amino Acids in the Cortex of the Rat: Monitoring by Microdialysis of Early Ischemic Changes

Regenerated rat skeletal muscle after periodic contusions

Contact Info

Product

Resources

About