Histochemical Demonstration of Oxidative Enzymes in Human Salivary Glands

Abstract

Histochemical Demonstration of Oxidative Enzymes in Human Salivary Glands. Recently, the salivary glands function from biochemical, physiological and histochemical standpoints have been rather highlighted. Many investigators have come to try a histochemical detection of enzymes participating in various metabolic pathway in the salivary glands of experimental animals and humans. The previous study of this series dealt with the histochemical demonstration of various hydrolytic enzymes and dehydrogenases in major salivary grands (KAWAKATSU et al. 1960 a, b, 1961 a, b, 1962 ad). It covered a comparison of the presence of enzymes in the serous and mucous cells of animal salivary glands. It also refered to relations between the localization of oxidative enzymes and their mitochondrias deposition in the electron microsrope. The present experiments were a histochemical approach to the distribution of oxidative enzymes in human major salivary glands.

Immunohistochemical evaluation of different filament proteins in human salivary glands.

“…The polarity of keratin localization in ED has already been found in SD and ED of animal specimens, and similar findings in which keratin staining strongly exists in basal cells of ducts were also obtained from animal salivary glands (34). Cytological findings in ductal epithelial cells in salivary glands have shown that mitochondrial accumulation and succinate dehydrogenase activity were situated in the basal aspects (20,21), whereas intermediate sized filament proteins were contained at the luminal border, and those findings were interesting features. It has been reported that keratin positive cells located at the basal layer of salivary gland ED were identified as myoepithelial cells (5, 9, 10), however the author did not recognize myoepithelial cells, because myoepithelial cells were generally localized in the periphery of acinar compartments and were not in the surrounding excretory ducts.…”