Histochemical demonstration of dehydrogenase activity in the cuticle of cestodes

Histochemistry of Hymenolepis microstoma (Cestoda: Hymenolepididae)

“…His findings closely parallel the histochemical findings in the present investigation; however, he had not included observations on the cuticle or the anterior regions of the worm. Rothman & Lee (1963) clearly demonstrate dehydrogenase activity in the basal layer of the cuticle of a number of species of Hymenolepis. Threadgold (1962), while studying the ultrastructure of the cuticle and subcuticle of Dipylidium canium, and Lumsden (1965) in describing the ultrastructure of the cuticle of H. diminuta, describe cells whose protoplasmic processes, containing mitochondria, traverse the subcuticular muscle layers and basement membrane of the cuticle and come in contact with the cuticle.…”

“…With the exception of the reports by Rothman & Lee (1963) and Waitz & Schardein (1963), histochemical investigations pertaining to the distribution of succinic dehydrogenase in tissues of members of the genus Hymenolepis have been based on formazan deposits from tetrazolium salts other than NBT or MTT-Co. The advantages of the former compound in dehydrogenase studies are reviewed by Nachlas, et al (1957) and Pearse (1960).…”

“…While certain aspects of the biology of the worm have been investigated (see Bogitsh (1963) for a review), little work has been done on the enzymology of this form. Bogitsh (1963) has reported on the histochemical localization of acid and alkaline phosphatases in the cuticule and subcuticle, and Rothman & Lee (1963) have demonstrated the presence of succinic dehydrogenase in the basal layer of the cuticle of H. microstoma. The latter report is the only investigation in the literature referring to succinic dehydrogenase activity in H. microstoma, and it is concerned only with the cuticular regions of the worm.…”

“…Briefly, then, the goals of this paper are: (1) To present material supplementing the report of Rothman & Lee (1963) by analysing histochemically the distribution of succinic dehydrogenase throughout the entire worm. (2) To present quantitative data pertaining to the distribution of the enzyme in various regions of the strobila to determine whether or not a correlation exists between the pattern of distribution and the level of development of the proglottids.…”

Histochemical Localisation of Lactate Dehydrogenase in Schistosoma Spindale

“…Adult F. hepatica showed the presence of Lactate dehydrogenase activity. Histochemical demonstration of Lactate dehydrogenase activity was observed in the cuticle of cestodes by Rothman and Lee(1963) [18]. Histochemical localization of lactate dehydrogenase was carried out in Paramphistomum cervi (Trematoda: Paramphistomidae by Patil and Rodgi(1976) [19].…”