Hymenolepis diminuta (order cyclophyllidea): Histochemical localization of glycogen, neutral lipid, and alkaline phosphatase in developing worms

Mayberry, Lillian F.; Tibbitts, F. Donald

doi:10.1007/bf00259486

Cited by 7 publications

(10 citation statements)

References 33 publications

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“…This nonuniform activity of AlPase along the length of the worm may indicate a selective function in different regions. Mayberry and Tibbitts (1972) also found an increased intensity of AlPase in mature and gravid proglottides than in immature ones of Hymenolepis diminuta. Ohman-James (1968) related the increased AlPase activity in the anterior end of Diphyllobothrium dendriticum to an increased host-parasite contact in that part of the parasite.…”

Section: Discussionmentioning

confidence: 62%

“…However, there is experimental evidence that host-induced variations in histochemical reaction of trematode tissues occur (Watertor and Van Landingham, 1976) and this aspect merits further study. Though no precise function of AlPase is known for these parasites, it has been suggested it is involved in the active transport and/or digestion (Arme and Read, 1970;Mayberry and Tibbitts, 1972). The same functions are, therefore, suggested for AlPase in R. (R.) johri.…”

Section: Discussionmentioning

confidence: 92%

“…She, however, did not observe the AlPase in these canals. Lee and Tatchell (1964) and Mayberry and Tibbitts (1972) found intense activity of AlPase in the dorsal excretory canal of Anoplocephala perfoliata and H. diminuta, respectively. Similar observations were reported by Erasmus (1957) on the irregular AlPase activity along the ventral excretory canals of Moniezia expansa while Bogitsh (1963) did not find any variation in the intensity of enzyme reaction along the walls of the canal of H. microstoma.…”

Section: Discussionmentioning

confidence: 96%

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Histochemical studies on Raillietina (Raillietina) johri (Cestoda: Davaineidae). I. Nonspecific and specific phosphatases

Roy

1979

J. Helminthol.

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Certain phosphatases have been localized by histochemical techniques in various tissues of a pigeon cestode, Raillietina (Raillietina) johri. Acid phosphatase (AcPase), alkaline phosphatase (AlPase) and adenosine triphosphatase (ATPase) were present in almost all structures: tegument; subtegumental muscles; subtegumental cells; excretory canal; tsetes; sperm ductules; vas deferens; cirrus sac; cirrus; ovary; receptaculum seminis; vagina; vitelline gland cells; oocytes; uterus; embryonated eggs. AlPase was absent in parenchyma, spermatocytes, spermatids and spermatozoa. AlPase activity was more intense in the tegument of mature gravid proglottides. AcPase and ATPase were visualized in various stages of spermatogenesis of the parasite. ATPase activity was also observed in chromosomes. 5'-nucleotidase (AMPase) activity was restricted to embryonated eggs only. Functional significance of these phosphatases is discussed.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 96%

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Histochemical studies on Raillietina (Raillietina) johri (Cestoda: Davaineidae). I. Nonspecific and specific phosphatases

Roy

1979

J. Helminthol.

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“…OHMAN-JAMES (1968) reported AcPase to be present and AlPase to be absent in the excretory canals of the plerocercoid and adult of D. dendriticum. LEE & TATCHELL (1964) and MAYBERRY & TIBBITTS (1972) reported a high concentration of AlPase in the dorsal excretory canals of Anoplocephala perfoliata and H. diminuta, whereas ERASMUS (1957 b) reported it to be irregularly distributed along the ventral excretory canals of Moniezia expansa. BOGITSH (1963) and ROY (1979) could not detect any change in the intensity of these enzymes in H. microstoma and R. (R.)johri respectively.…”

Section: Disctss1onmentioning

confidence: 95%

“…It has been established that the cestode tegument is metabolically active and can take up, as well as digest, nutrients through active transport (SMYTH, 1969; DIKE & READ, 1971), which is accomplished by phosphohydrolases (ROY, 1979). The distribution of AcPase and AlPase in the tegument of A. lahorea in high concentrations indicates a close parallel between the distribution of phosphatases and the sites involved in active transport, presumably through AlPase (ARME & READ, 1970;MAYBERRY & TIBBITTS, 1972;ROY, 1979). Obviously these enzymes play the same role in A. lahorea since it occupies the same niche as H. diminuta and Moniezia expansa.…”

Section: Disctss1onmentioning

confidence: 99%

Histochemical localization of phosphomonoesterases in Avhellina lahorea Woodland, 1927 (Cestoda: Anoplocephalida)

Farooq¹,

Farooqi²

1984

J. Helminthol.

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Non-specific and specific phosphatases have been histochemically localized in the tissues of Avitellina lahorea, an intestinal parasite of sheep and goats. Large quantities of acid phosphatase, alkaline phosphatase and adenosine triphosphatase were observed in almost all organs except the parenchyma where there were moderate amounts of acid phosphatase and no alkaline phosphatase; the reproductive ducts contained moderate amounts of alkaline phosphatase. 5-nucleotidase was observed only in the uterus, egg pouches and eggs and glucose-6-phosphatase activity was restricted to the tegument. The probable functions of these moieties at different sites are discussed.

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Qualitative distribution of neutral lipids and phospholipids in Hymenolepis microstoma from the cysticercoid to the egg producing adult

Chowdhury

Rycke

1976

Z. Parasitenk

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The distribution of neutral lipids and phospholipids in Hymenolepis microstoma has been studied using Fettrot, Sudan Black B, Sudan IV and copper phthalocyanin staining techniques. In the cysticercoid, neutral lipids are found in the outer membrane, the lining of the cysticercoid cavity, the tegument of the larval worm and the calcareous corpuscles. A decreasing gradient of phospholipids is found starting from the acellular layer, through the circular fibrous layer, the longitudinal fibrous layer, the adjacent dense zone and ending with the lining of the cysticercoid cavity. Phospholipids are also found in the calcareous corpuscles and the tegument of the larval worm. In the young adult (3 days p.i.) fat globules are first seen to accumulate in the last 2-3 proglottids. Until the 6th day p.i. they are found in the posterior third of the worm, surrounding developing gonads, but mostly concentrated along the transverse line. The mature proglottids contain fat, (a) in both granular and globular forms: in the folds of the uterus, sperm ducts, cirrus pouch and tegument (proximal cytoplasm), (b) in a diffuse form: in the vitellaria, ovary, testes and the tegument (distal cytoplasm). Pre-gravid and gravid proglottids show the largest fat globules. From the cleaving embryo to the fully developed oncosphere the concentrations of neutral lipids and phospholipids vary in form, intensity and location. In all strobilar forms of the parasite neutral lipids and phospholipids are found in the tegument and calcareous corpuscles. Although in H. microstoma lipid droplets are found in the excretory canals, all lipids in the proglottids are not absolutely waste products. From the results it would appear that they play a role in the maturation of gonads and transformation of the fertilized ovum to the oncosphere.

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Hymenolepis diminuta (order cyclophyllidea): Histochemical localization of glycogen, neutral lipid, and alkaline phosphatase in developing worms

Cited by 7 publications

References 33 publications

Histochemical studies on Raillietina (Raillietina) johri (Cestoda: Davaineidae). I. Nonspecific and specific phosphatases

Histochemical studies on Raillietina (Raillietina) johri (Cestoda: Davaineidae). I. Nonspecific and specific phosphatases

Histochemical localization of phosphomonoesterases in Avhellina lahorea Woodland, 1927 (Cestoda: Anoplocephalida)

Qualitative distribution of neutral lipids and phospholipids in Hymenolepis microstoma from the cysticercoid to the egg producing adult

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