Histochemical and morphological aspects of fresh frozen bone: a preliminary study

Ponte, Francesco Saverio De; Cutroneo, Giuseppina; Falzea, R; Rizzo, Giuseppina; Catalfamo, Luciano; Favaloro, Angelo; Vermiglio, Giovanna; Runci, Michele; Centofanti, Antonio; Anastasi, Giuseppe

doi:10.4081/ejh.2016.2642

Cited by 17 publications

(20 citation statements)

References 23 publications

(20 reference statements)

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“…In other studies, the resorption rates reported confirmed the excellent volumetric stability and great flexibility of autologous bone blocks in all clinical situations, despite intra-and post-operative complications [25][26][27][28].…”

Section: Introductionsupporting

confidence: 67%

Comparative Investigation of Cutting Devices on Bone Blocks: An SEM Morphological Analysis

et al. 2019

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Background: Bone regeneration is a reliable technique when the bone volume is insufficient to provide a functional and aesthetic outcome in surgery and implantoprosthesis procedures. When bone blocks are used but do not match the shape of the defect, the block must be adapted. The aim of our research was to evaluate, by Scanning Electron Microscopy (SEM) morphological observation, how different cutting devices modify the bone surface. Method: Four equine bone blocks were divided into 15 cubic shape samples with ultrasonic and sonic tips, as well as diamond, tungsten carbide, and Lindemann burs. The uncut surface of the obtained bone block was used as a control. Two observers independently analyzed the SEM observation recording, including cut precision, depth of incision, thermal damages, and presence of bone debris. For each group, sharpness, depth, carbonization, and bone debris were expressed as mean values. Results: The osteotomy performed with an ultrasonic tip shows the best results, preserving the bone morphology in both quantitative and qualitative analyses. The bone surface appeared sufficiently clean from debris and showed a reduced presence of carbonization. Conclusion: The shaping of the bone block as in vivo osteotomy respects the bone morphology and allows it to achieve the relevant biological and clinical outcome.

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Section: Introductionsupporting

confidence: 67%

Comparative Investigation of Cutting Devices on Bone Blocks: An SEM Morphological Analysis

et al. 2019

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“…Tissue biopsy samples (1-2 mm 3 ) from the explanted ADM were obtained immediately after interruption of the blood supply during reconstructive surgeries. As controls, acellular dermal matrix before implantation and one sample of the native dermis of the skin were examined.…”

Section: Methodsmentioning

confidence: 99%

“…The removal of cellular components from the connective tissue leaves the water-insoluble matrix, generally known as the decellularized or acellular matrix / scaffold / graft. Currently, acellular matrices are used for various purposes and they have been studied extensively for their potential roles in regenerating tissues or organs in vascular surgery, 1 abdominal surgery, 2 dentistry and facial surgery, 3 or orthopedics. 4 , 5 The acellular matrix generates physiological cues that mimic the native tissue microenvironment and is, therefore, more appropriate for re-functionalization and regeneration of a tissue compared with commonly used synthetic biomaterials.…”

Section: Introductionmentioning

confidence: 99%

What happens to an acellular dermal matrix after implantation in the human body? A histological and electron microscopic study

et al. 2018

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Acellular matrices are used for various purposes and they have been studied extensively for their potential roles in regenerating tissues or organs. The acellular matrix generates physiological cues that mimic the native tissue microenvironment. Acellular dermal matrix (ADM) is a soft connective tissue graft generated by a decellularization process that preserves the intact extracellular skin matrix. Upon implantation, this structure serves as a scaffold for donor-side cells to facilitate subsequent incorporation and revascularization. In breast reconstruction, ADM is used mainly for lower pole coverage and the shaping of a new breast. It helps control the positioning of the implant in the inframammary fold, and prevents the formation of contractile pseudocapsule around the breast implant. In this study, we provide a comprehensive histological description of ADM used for human breast reconstruction over the course of several months following implementation. Using immunohistochemical methods (a panel of 12 antibodies) coupled with optical and transmission electron microscopy, we confirmed that the original acellular dermal matrix became recolonized by fibroblasts and myofibroblasts, and also by various other free cells of the connective tissue (lymphocytes, macrophages and multinucleated giant cells, granulocytes, mast cells) after implantation into the patient’s body. Within the implanted ADM, there was a relatively rapid ingrowth of blood vessels. Lymphatic vessels were only detected in one case 9 months after the implantation of the ADM. These results suggest that lymphangiogenesis is a longer process than angiogenesis.

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“…From allograft studies in rhesus monkeys, it is known that non‐decalcified freeze‐dried human allograft is superior to decalcified allografts in stimulating new bone formation . OC‐immunoreactive cells were also observed after application of fresh frozen bone for sinus lift after 6 months …”

Section: Discussionmentioning

confidence: 99%

Histological and immunohistochemical comparison of two different allogeneic bone grafting materials for alveolar ridge reconstruction: A prospective randomized trial in humans

Solakoglu

Götz

Heydecke

et al. 2019

Clin Implant Dent Rel Res

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BackgroundPreclinical studies have hypothesized a possible immunological reponse to allogeneic materials due to detection of remnants of potential immunogenic molecules. However, their impact on integration, bone remodeling and immunological reaction after the augmentation procedure is largely unknown and a direct correlation of analytical data and evaluation of human biopsies is missing.PurposeThe present study aimed to compare two commercially available allogeneic materials regarding their content of cellular remnants as well as the bone remodeling, and integration and potential immunologic reactions on a histological and immunohistochemical level, integrating also in vitro analytical evaluation of the specific batches that were used clinically.Materials and MethodsTwenty patients were randomly assigned to treatment with Maxgraft or Puros for lateral ridge augmentation in a two‐stage surgery. After a mean healing period of 5 months, implants were placed and biopsies were taken for histological, immunhistochemical, and histomorphometrical evaluation regarding bone remodeling and inflammation, protein concentrations in vitro and the presence of MHC molecules of the same batches used clinically.ResultsNo differences in clinical outcome, histological, immunohistochemical, and in vitro protein analysis between the two bone grafting materials were observed. Active bone remodeling, amount of newly formed bone, and residual grafting material was independent of the materials used, but varied between subjects. MHC1 residues were not detected in any sample.ConclusionsWithin the limitations of this study, both tested materials yielded equivalent results in terms of clinical outcome, new bone formation, and lack of immunological potential on a histological and immunohistochemical level.

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Histochemical and morphological aspects of fresh frozen bone: a preliminary study

Cited by 17 publications

References 23 publications

Comparative Investigation of Cutting Devices on Bone Blocks: An SEM Morphological Analysis

Comparative Investigation of Cutting Devices on Bone Blocks: An SEM Morphological Analysis

What happens to an acellular dermal matrix after implantation in the human body? A histological and electron microscopic study

Histological and immunohistochemical comparison of two different allogeneic bone grafting materials for alveolar ridge reconstruction: A prospective randomized trial in humans

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