Histochemical analyses of glycoconjugates and antimicrobial substances in goat labial glands

Habata, Isao; Yasui, Tadashi; Fujimori, Osamu; Meyer, Wilfried; Tsukise, Azuma

doi:10.1016/j.acthis.2011.08.006

Cited by 5 publications

(4 citation statements)

References 60 publications

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“…In domestic animals, the histological structure of the lips is; outer external layer of skin and lined internally by mucous membrane containing in between them the orbicularis oris muscle, the labial vessels, nerves, connective tissue, and labial glands (Nickel et al, 1979; Saxena et al, 2007). In agreement to the present report, the labial glands of Rahmani sheep are seromucous in nature similar to that of goat and buffalo (Habata et al, 2012; Mahdy et al, 2020; Mansour et al, 2007). The Rahmani sheep labial glands may play important role in the mucosal immune system similar to that of goats as described previously by Habata et al (2012).…”

Section: Discussionsupporting

confidence: 92%

“…In agreement to the present report, the labial glands of Rahmani sheep are seromucous in nature similar to that of goat and buffalo (Habata et al, 2012; Mahdy et al, 2020; Mansour et al, 2007). The Rahmani sheep labial glands may play important role in the mucosal immune system similar to that of goats as described previously by Habata et al (2012).…”

Section: Discussionsupporting

confidence: 92%

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Histomorphological investigations on the lips of Rahmani sheep (Ovis aries): A scanning electron and light microscopic study

Madkour

Mohammed

2020

Microscopy Res & Technique

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This study was conducted to provide comprehensive information on the anatomical and histological features of the upper (UL) and lower (LL) lips of Rahmani sheep by gross examination, morphometric analysis in addition to Light and scanning electron microscope (SEM). Samples from normal healthy adult male sheep heads were collected directly after slaughtering. UL and LL were dissected, and specimens were collected for both light and SEM. The UL was longer approximately by one‐fold and thicker by one‐fold at the median and the oral angle areas, and by one‐ and half‐folds at the paramedian area than the LL. The free border of both lips was characterized rostrally by the presence of labial projections. By SEM the edges of the inner aspect of the lips and of the philtrum were distinguished by labial projections. These projections which surrounding the philtrum subdivided into polygonal areas with numerous keratinized scales especially at the apical parts which increased dorsally toward the nostril. Most of the openings of the upper labial salivary glands were volcanic crater‐shaped while that of the lower labial salivary glands were various shapes; round folded margin, rosette and whirlpool shaped. Histologically, the shape of the projection or papillae differs at the median and paramedian areas of the UL than the LL. However, there was no differences near the oral angle area. In conclusion, the shape, size and amount of keratinization of the papillae may offer efficiency to the lips during feeding process.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Histomorphological investigations on the lips of Rahmani sheep (Ovis aries): A scanning electron and light microscopic study

Madkour

Mohammed

2020

Microscopy Res & Technique

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“…However, the cytological and glycoconjugate histochemical observations of the secretory cells obtained differed from those of the salivary glandular cells. 26 , 43 , 44 According to Martin et al, three types of secretory granules may be present in the acinar cells of canine lacrimal and third eyelid glands. 30 The structural features of the granule type in tubular cells and the most common granule type in acinar cells correspond to our results except for the electron density of the latter.…”

Section: Discussionmentioning

confidence: 99%

Morphological and histochemical characterization of the secretory epithelium in the canine lacrimal gland

et al. 2021

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In the present study, the expression of secretory components and vesicular transport proteins in the canine lacrimal gland was examined and morphometric analysis was performed. The secretory epithelium consists of two types of secretory cells with different morphological features. The secretory cells constituting acinar units (type A cells) exhibited higher levels of glycoconjugates, including β-GlcNAc, than the other cell type constituting tubular units (type T cells). Immunoblot analysis revealed that antimicrobial proteins, such as lysozyme, lactoferrin and lactoperoxidase, Rab proteins (Rab3d, Rab27a and Rab27b) and soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins (VAMP2, VAMP4, VAMP8, syntaxin-1, syntaxin-4 and syntaxin-6), were expressed at various levels. We immunohistochemically demonstrated that the expression patterns of lysozyme, lactoferrin, Raba27a, Rab27b, VAMP4, VAMP8 and syntaxin-6 differed depending on the secretory cell type. Additionally, in type T cells, VAMP4 was confined to a subpopulation of secretory granules, while VAMP8 was detected in almost all of them. The present study displayed the morphological and histochemical characteristics of the secretory epithelium in the canine lacrimal gland. These findings will help elucidate the species-specific properties of this gland.

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“…The expression and/or secretion of salivary components, which include antimicrobial substances, such as lysozyme, lactoperoxidase, lactoferrin, immunoglobulin A, and β-defensins, and glycoconjugates, such as mucin glycoproteins, as well as digestive enzymes such as α-amylase and lingual lipase, 41,42 have been substantially demonstrated in the salivary glands of humans, 3,[43][44][45][46][47][48] rodents, 36,49,50 and goats. [51][52][53] Meanwhile, the expression of antimicrobial substances differed among glands, with high expressions of lysozyme in the parotid and zygomatic glands and lactoperoxidase in the parotid gland. Thus, our results emphasize that canine major salivary glands are functionally subdivided, such as for food digestion or the maintenance of the intraoral environment.…”

Section: Discussionmentioning

confidence: 99%

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties

Gomi

Osawa

Uno

et al. 2017

J Histochem Cytochem.

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The comparative structure and expression of salivary components and vesicular transport proteins in the canine major salivary glands were investigated. Histochemical analysis revealed that the morphology of the five major salivary glands-parotid, submandibular, polystomatic sublingual, monostomatic sublingual, and zygomatic glands-was greatly diverse. Immunoblot analysis revealed that expression levels of α-amylase and antimicrobial proteins, such as lysozyme, lactoperoxidase, and lactoferrin, differed among the different glands. Similarly, Rab proteins (Rab3d, Rab11a, Rab11b, Rab27a, and Rab27b) and soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins VAMP4, VAMP8, syntaxin-2, syntaxin-3, syntaxin-4, and syntaxin-6 were expressed at various levels in individual glands. mmunohistochemistry of Rab3d, Rab11b, Rab27b, VAMP4, VAMP8, syntaxin-4, and syntaxin-6 revealed their predominant expression in serous acinar cells, demilunes, and ductal cells. The VAMP4/syntaxin-6 SNARE complex, which is thought to be involved in the maturation of secretory granules in the Golgi field, was found more predominantly in the monostomatic sublingual gland than in the parotid gland. These results suggest that protein expression profiles in canine salivary glands differ among individual glands and reflect the properties of their specialized functions.

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Histochemical analyses of glycoconjugates and antimicrobial substances in goat labial glands

Cited by 5 publications

References 60 publications

Histomorphological investigations on the lips of Rahmani sheep (Ovis aries): A scanning electron and light microscopic study

Histomorphological investigations on the lips of Rahmani sheep (Ovis aries): A scanning electron and light microscopic study

Morphological and histochemical characterization of the secretory epithelium in the canine lacrimal gland

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties

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