Histomorphological investigations on the lips of Rahmani sheep (<scp><i>Ovis aries</i></scp>): A scanning electron and light microscopic study

Mohamed

Abdalla

et al. 2021

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The present study was implemented to provide comprehensive information on the developmental sequence of the proventriculus of Muscovy ducks by gross examination, macro-micrometric analysis and by using light microscope, scanning electron microscope (SEM) and transmission electron microscope (TEM). Samples from 55 healthy post-hatching Muscovy ducks of both sexes ranging from 1 to 60 days old. The proventriculus began cranially opposite to the cranial end of the liver at 1-15 days old, but in front this level at 30 and 60 days old. Morphometrically, the length of the proventriculus was increased by about four folds while weight by 19 folds at 60 days old when compared with those at one day old. Scanning electron microscopy of the proventricular lumen at one day old exhibited numerous small mostly rounded irregularly distributed openings of the glands, but uniformly distributed and surrounded by closely packed concentrically arranged mucosal folds resembling a rosette shape at the older ages. Histologically, in all studied stages, rounded, elongated oval or polymorphic shaped lobules of the proventricular glands were occupied within the muscularis mucosa. The tubuloalveolar secretory units lined with secretory (oxyntico-peptic) cells with variable shapes had secretory granules increased by the development. Numerous argyrophilic endocrine cells were demonstrated away from the glandular lumen at older ages. Transmission electron microscopy revealed that the cytoplasm of the secretory cells contained homogeneously electron-dense granules at a young age, but two types of these granules could be recognized at 60 days old. In conclusion, this study provides a wide difference in the morphometric and the structure of the proventriculus from one day to 60 days old. This difference between the examined age-stages may be related to the feeding strategy (behavior) and the functional adaptations from the young to the older ages.

Section: Digital Coloring Of Scanning and Transmission Electron Microscopic Imagesmentioning

confidence: 99%

Developmental stages and growth of the proventriculus of post‐hatching Muscovy duck: Light and electron microscopic study

Mohamed

Abdalla

et al. 2021

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“…(Photo Filter 7.2.1 is a free download program, the coloring process performed by the determination a certain area within the image by a polygon tool, after that, choose Filter, then color, and color layer or colorized.) Several authors used this method (Madkour, Mohamed, Abdalla, & Ahmed, 2021; Madkour & Mohammed, 2021; Madkour, Mohammed, Radey, & Abdelsabour‐Khalaf, 2021; Soliman & Madkour, 2021).…”

Section: Methodsmentioning

confidence: 99%

Scanning electron microscopy of the nasal skin in different animal species as a method for forensic identification

Abdelsabour-Khalaf

2021

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The current study aims to provide a new method for the identification of animal species by using the scanning electron microscopic study on the nasal skin around the nostril. The reference databases for animal identification using traditional ways (i.e., hair and bone analyses) were somewhat available. This study was conducted on the nasal skin of seven different animal species: large ruminants (Saidi buffalo and frozen Angus cattle); small ruminants (Egyptian goat and Rahmani sheep); camels (one‐hump dromedary camel); equines (African domestic donkey and Arabian horse); carnivores (farm domestic cat, Egyptian Baladi dog, and red fox); swine (Danish Landrace pig); and rodents (albino rat and New Zealand white rabbit). The nasal skin was divided by grooves into different shaped raised areas in some examined animals, the mean diameter of these areas and width of the grooves of each animal species were recorded. The characteristic differential features of the nasal skin of each animal species have been documented. Few openings of the nasolabial glands were demonstrated in large ruminants and swine, which were H letter‐shaped in buffaloes, oval‐shaped in cattle, and whirlpool‐shaped with several layers of folds on their margin in pig. Moreover, the openings of the lateral nasal glands were whirlpool, ovoid, and oval shaped in goats, sheep, and camels, respectively. In conclusion, the present study proved that the skin around the nostrils plays an important role as means of identification in forensic investigations and improves the field of veterinary forensic medicine in general, which is not well‐developed in comparison with human forensics.

“…This procedure clarified the hair cuticle scale patterns of the tested animal and the margin type of the cuticles. This technique was performed as previously described by Madkour and Mohammed (2021); Madkour, Mohamed, et al (2022); Madkour, Mohammed, et al (2022); and Soliman and Madkour (2021).…”

Section: Coloring Of Sem Imagesmentioning

confidence: 99%

Performance scanning electron microscopic investigations and elemental analysis of hair of the different animal species for forensic identification

Abdelsabour-Khalaf

2022

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Veterinary forensics have attracted less attention compared with human forensics. Animal hair morphological examination using scanning electron microscopy (SEM) and hair mineral analysis using energy-dispersive X-ray fluorescence (ED-XRF) provide reference databases for animal hair identification used in forensic investigations. This study was performed on four different animal species: large ruminants (buffalo and cattle), small ruminants (goat and sheep), carnivores (cat and dog), and equines (donkey and horse). The hair scale pattern, scale margin type, and distance between scales were identified. The hair scale pattern was imbricate in large ruminants, goat, and horse; coronal (crown-like) in carnivores and donkey; and spinous in sheep. The morphometric measurements, including cuticle layer thickness, cuticle scale height, cortex diameter, and hair shaft width were recorded. The highest cuticle thickness was found in horse, and the highest cuticle hair scale was detected in cattle. Moreover, the largest cortex diameter was measured in buffalo, whereas the smallest one was in cat. With regard the content of hair elements, some elements were specific for the hair of certain tested animals, such as bromine in sheep and magnesium and phosphorus in buffalo. In addition, vanadium and titanium were found only in cattle and dog, and the latter element was also detected in sheep. In conclusion, this study provides two techniques for animal hair identification that can be used in forensic investigations. Research highlights• Hair scale pattern was imbricate in (large ruminants, goat, and horse); coronal in (carnivores and donkey); and spinous in (sheep) with different scale margins type.• Hair shaft width in all tested animal species ranged from 23.78 ± 1.24 μm to 85.51 ± 1.14 μm.