Histamine release test in the diagnosis of human hydatidosis

Aceti, Antonio; Celestino, D; Teggi, Antonella; Caferro, Marta; Pennica, Alfredo; Grilli, Anastasio; Sebastiani, A.; Rosa, F. De

doi:10.1111/j.1365-2222.1989.tb02392.x

Cited by 16 publications

(9 citation statements)

References 18 publications

(21 reference statements)

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“…Wc have reported that in basophils from hydatid pa tients the antigen-induced histamine release correlates neither with serum nor with cell-bound specific IgE [8], From the present study it is apparent that E. granulosus in fection induces both generation of histamine releasing factor and production of IgE that can bind this cytokine. Thus, it is possible that these two events can influence the response of basophils to antigenic challenge.…”

Section: Discussioncontrasting

confidence: 60%

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Spontaneous in vitro Generation of Histamine Releasing Factor from Mononuclear Cells of Patients with Hydatidosis

Aceti¹,

Celestino²,

Teggi³

et al. 1992

Int Arch Allergy Immunol

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We have shown that Echinococcus granulosus infection can induce an enhanced sensitivity of basophils to IgE-dependent stimuli. To determine whether a cytokine, histamine releasing factor, could account for it, we evaluated 35 patients with active hydatidosis, 5 patients with past E. granulosus infection, and 20 normal volunteers. The spontaneous production of a factor in vitro that provoked the release of histamine from basophils was observed by mononuclear cells from patients with active disease, but not by those from subjects with past infection or from normal individuals. The histamine releasing factor was found to activate basophils through surface-bound IgE. It is concluded that E. granulosus infection induces both generation of histamine releasing factor and production of IgE that can bind this cytokine.

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Section: Discussioncontrasting

confidence: 60%

“…Indeed, this hypcrrcleasable state makes it possible to obtain a high response to appropriate antigen, even though levels of cell-bound IgE are low [8].…”

Section: Introductionmentioning

confidence: 99%

Spontaneous in vitro Generation of Histamine Releasing Factor from Mononuclear Cells of Patients with Hydatidosis

Aceti¹,

Celestino²,

Teggi³

et al. 1992

Int Arch Allergy Immunol

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“…basophil) depends also on its 'releasability', that is the intrinsic capacity of the cell to release mediators in response to a variety of immunologic and non-immunologic stimuli [1], We have studied the antigen-specific histamine release from blood basophils in subjects with hydatidosis [2,3]. In our patients, a high rate of histamine liberation was noted in all cases, even though the levels of serum reaginic antibodies were undetectable.…”

mentioning

confidence: 93%

“…Histamine release experiments were carried out following the procedure previously described [3]. Histamine content was as sessed by the automated fluorometric assay [4], The following stimuli were used for challenge: calcium ionophore A23187 (Calbiochem); formyl-methionylleucyl-phenylalanine (f-met peptide; Sigma); goat anti-human IgE (Sigma), and hydatid antigen, pre pared according to the method previously described [3]. IgE was dissociated from basophils of 12 patients with hydatidosis by acetate elution [5].…”

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Basophil Releasability in Human Hydatidosis

Aceti¹,

Celestino²,

Caferro³

et al. 1990

Int Arch Allergy Immunol

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We studied immunoglobulin E (IgE)- and non-IgE-mediated releasability in basophils from 31 patients with hydatidosis. Histamine release to non-IgE-dependent stimuli did not differ significantly between normal individuals and patients with hydatidosis. On the contrary, an increased histamine liberation was obtained by challenging basophils from hydatid patients with anti-human IgE. It is concluded that Echinococcus granulosus infection induces an enhanced sensitivity of basophils to IgE-dependent stimuli.

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Echinococcosis and allergy

Vuitton

2004

Clinic Rev Allerg Immunol

108

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The larval stages of Echinococcus granulosus and E. multilocularis are involved in parasitic diseases in humans: cystic echinococcosis (CE) ("hydatid disease") and alveolar echinococcosis (AE), respectively. Both diseases and parasites have tight links with allergy because of the immunological characteristics that contribute to maintain the larvae in their human host as well as their potential in inducing clinical anaphylactic reactions in some patients. Clinical observations in patients and data obtained from mass screenings in various countries have identified both forms of echinococcosis as "polar diseases," i.e., diseases where immunological background of the patients was related to the clinical presentation and course. In particular, abortive cases (i.e., spontaneous cures) have been found in many subjects in endemic areas. On the other hand, immune suppression was associated with severe disease. AE especially might be considered as an opportunistic infection. Experimental and clinical studies have shown that Th1-related immune response was associated with protection and Th2-related response was associated with parasite growth. Genetic characteristics of the host are related to both occurrence and severity of AE and are associated with the extent of IL-10 secretion, which is a major feature of chronic progressing echinococcosis. Anaphylactic reactions, including urticaria, edema, respiratory symptoms, and anaphylactic shock due to spontaneous or provoked rupture of the parasitic cyst, are well known in CE. Anaphylactic reactions in AE are far less frequent, and have been observed in rare cases at time of metastatic dissemination of the parasitic lesions. Echinococcus-specific IgE is present in most of the patients and associated with severity. Specific histamine release by circulating basophils stimulated with E. granulosus antigens is present in all patients with CE and AE. Echinococcus allergens include (1) AgB 12-kDa subunit, a protease inhibitor and a potent Th2 inducer; (2) Ag5, a serine protease; (3) EA 21, a specific cyclophilin, with a homology with other types of cyclophilins; (4) Eg EF-1 beta/delta an elongation factor, with a homology with Strongyloides stercoralis EF that shares the same IgE epitope. A clinical cross-reaction with Thiomucase, a mucopolysaccharidase used in arthritis treatment, has recently been published. However, despite the potential risk of allergic reactions, the dogma "never puncture a hydatid cyst" is no longer valid. International experience of therapeutic technique of "puncture, aspiration, injection, re-aspiration" of hydatid cysts developed at the beginning of the 1980s has proved to be successful in a variety of selected indications that have been reviewed by WHO recommendations. A better understanding of the immunological background of echinococcosis in humans has led to new therapeutic developments, such as immunomodulation using interferon alpha. Th2-driven immunological response and IL-10-related tolerance state are common characteristics of atopic allergy and echi...

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Histamine release test in the diagnosis of human hydatidosis

Cited by 16 publications

References 18 publications

Spontaneous in vitro Generation of Histamine Releasing Factor from Mononuclear Cells of Patients with Hydatidosis

Spontaneous in vitro Generation of Histamine Releasing Factor from Mononuclear Cells of Patients with Hydatidosis

Basophil Releasability in Human Hydatidosis

Echinococcosis and allergy

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