Histamine H3 and H4 receptors are expressed on distinct endocrine cell types in the rat fundic mucosa

Morini, Giuseppina; Becchi, G; Shenton, Fiona C.; Chazot, Paul L.; Grandi, Daniela

doi:10.1007/s00011-007-0628-9

Cited by 28 publications

(25 citation statements)

References 3 publications

(5 reference statements)

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“…Our histochemical data add the collecting duct cells to the growing list of non-neuronal H 3 R-expressing cells already reported [7][8][9], thus providing further evidence for a role of H 3 R in mediating non-neuronal histamine effects. The collecting duct plays a pivotal role in kidney function and homeostasis by regulating ions and water transport.…”

Section: Discussionsupporting

confidence: 73%

“…Immunoperoxidase staining for H 3 R was performed on 5 µm kidney sections for formalin-fixed tissue from 24 male 8-week-old Wistar rats (12 non-diabetic and 12 diabetic animals). Renal sections were incubated overnight with anti-H 3 R (349-358) (2 µg/ml) [7], followed by a three-layer streptavidin-biotin-peroxidase complex staining method. Photomicrographs were acquired randomly with a digital camera connected to a light microscope equipped with a x40 objective (Leica DM750 To confirm the absence of false staining, tissue was also screened in the absence of primary antibodies and following pre-incubation with (349-358) peptide (data not shown).…”

Section: Methodsmentioning

confidence: 99%

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H3 receptor renal expression in normal and diabetic rats

et al. 2015

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Section: Discussionsupporting

confidence: 73%

Section: Methodsmentioning

confidence: 99%

H3 receptor renal expression in normal and diabetic rats

et al. 2015

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“…It has been largely demonstrated that H 3 R is mostly expressed in the central and, to a lesser extent, in the peripheral nervous system. Only a small number of experimental observations have showed its presence on non-neuronal cell types such as rodent fundic mucosa endocrine cells [26], cholangiocytes [37], pancreatic β-cells [38], and in the human bronchial epithelial cell line BEAS-2B [39]. Therefore, our data add a new non-neuronal localization of the H 3 R, contributing to the hypothesis that the H 3 R could also mediate nonneuronal histamine effects.…”

Section: Discussionsupporting

confidence: 64%

“…The final concentration of the organic solvent was less than 0.1%, which had no effect on cell viability. Antibodies H 3 R and H 4 R were detected by using the anti-hH 3 R (349-358) and the anti-hH 4 R (374-390) antibodies produced and validated for detecting both human and rodent H 3 R and H 4 R in the School of Biological and Biomedical Sciences, Durham University [21][22][23][24][25][26][27]. Briefly, anti-hH 3 R antibody was generated coupling the peptide corresponding to the amino acids RLSRDRKVAK Cys of the human and rat H 3 R to thyroglobulin by m-maleimidobenzoic acid N-hydroxysuccinimide ester coupling method as described previously [6] and antibodies were raised in rabbits to the resultant conjugate.…”

Section: Reagentsmentioning

confidence: 99%

Histamine receptor expression in human renal tubules: a comparative pharmacological evaluation

et al. 2015

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Objective and design The aim of this study is to evaluate the expression of the histamine receptors, particularly focusing on the H 4 R in human renal tubules. MaterialThe ex-vivo evaluation was carried on specimens from human renal cortex. Primary and immortalized tubular epithelial cells (TECs) and the HK-2 cell line were used as in vitro models.Treatment Cells were pretreated for 10 min with chlorfeniramine maleate 10 µM (H 1 R antagonist), ranitidine 10 µM (H 2 R antagonist), GSK189254 1 µM (H 3 R antagonist) or JNJ7777120 10 µM (H 4 R antagonist), and then exposed to histamine (3 pM -10 nM) for 30 min. MethodsThe ex-vivo evaluation on specimens from human renal cortex was performed by immunohistochemistry. The expression of histamine receptors on primary and immortalized TECs and the HK-2 cell line was evaluated at both gene (RT-PCR) and protein (immunocytofluorescence) levels. The pharmacological analysis was performed by TR-FRET measurements of second messenger (IP3 and cAMP) production induced by histamine with or without the selective antagonists.Results Our data revealed the presence of all histamine receptors in human tubules; however, only TECs expressed all the receptors. Indeed, histamine elicited a sigmoid dose-response curve for IP 3 production, shifted to the right by chlorpheniramine maleate, and elicited a double bell-shaped curve for cAMP production, partially suppressed by the selective H 2 R, H 3 R and H 4 R antagonists when each added alone, and completely ablated when combined together.Conclusions Herein, we report the identification of all four histamine receptors in human renal tubules.

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“…Another original contribution of our study is to provide an intra-nephron localization of the H putative distinct roles to the other histamine receptors in the body, the latter including exocrine and endocrine functions in salivary glands [44] and the GI tract [25], respectively.…”

Section: Renal H 4 Receptor Expression In Diabetic Ratsmentioning

confidence: 99%

Overexpression of histamine H4 receptors in the kidney of diabetic rat

Rosa

Grange²,

Pini

et al. 2012

Inflamm. Res.

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Objective and design: The renal expression of H 1 and H 2 receptors was previously demonstrated, while that of the H 4 receptor has been poorly investigated, thus the aim of this research was to investigate the expression of the H 4 receptor in the kidney of diabetic rats.Material or subjects: 24 8-week-old male Wistar rats Treatment: Diabetes was induced in 12 rats by a single i.v. injection of streptozotocin, and animals were sacrificed 6 weeks later.Methods: Kidneys were collected and processed for quantitative PCR or immunohistochemical analyses. To ascertain the renal topology of the H 4 receptor, colocalization experiments were performed with a series of markers.Results: H 4 receptor is expressed in healthy rats, although at a very low level, and is profoundly upregulated in diabetic animals. Immunohistochemical analysis revealed the highest immune-positivity in the medulla. Colocalization experiments revealed a close overlap in expression topology of the H 4 receptor and both Tamm-Horsfall glycoprotein and aquaporin 1 was observed.Conclusions: The results demonstrate, for the first time, that the H 4 receptor is expressed in the kidney mainly by resident renal cells of the loop of Henlé and that this receptor is significantly overexpressed in diabetic animals, thus suggesting a possible role in the pathogenesis of diabetes-associated renal disease.

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Histamine H3 and H4 receptors are expressed on distinct endocrine cell types in the rat fundic mucosa

Cited by 28 publications

References 3 publications

H3 receptor renal expression in normal and diabetic rats

H3 receptor renal expression in normal and diabetic rats

Histamine receptor expression in human renal tubules: a comparative pharmacological evaluation

Overexpression of histamine H4 receptors in the kidney of diabetic rat

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