Histamine determination in human urine using sub‐2 μm C18 column with fluorescence and mass spectrometric detection

Hogan, Anna-Marie; Crean, Conor; Barrett, Una Marie; Guihen, Elizabeth; Glennon, Jeremy D.

doi:10.1002/jssc.201101045

Cited by 13 publications

(7 citation statements)

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“…This was demonstrated with the detection of histamine in synthetic urine and a LOD of 76 pM was achieved, suggesting low matrix effect (Figure 5b). There are very few reports detailing the detection of histamine in urine, or other biological fluids, 16 and the majority of them describe the use of chromatography-based methods, with a wide range of detection limits reported, for example a LOD of 225 nM reported by Comas-Bastéet al, 48 and 40 pM by Hogan et al, 16 using the same technique.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Histamine is considered to be the most relevant BA due to its biological toxicity, which is enhanced in the presence of putrescine, cadaverine, tyramine, and phenylethylamine due to the inhibition of histamine-detoxifying enzymes. , The analytical determination of histamine is a complicated task due to the complexity of the matrices, , the structural similarities between the different BAs and the low concentration in biological fluids, with the normal concentration of histamine in blood, being 4.01 ± 3.4 nM and in urine 213.5 ± 178.9 nM . Current methods of detection are based on chromatography, including High Performance Liquid Chromatography coupled with mass spectrometry and fluorescence detection, as well as gas chromatography and thin layer chromatography . Less frequently, BAs are detected using ELISA, radioimmunoassays (RIA)nd capillary electrophoresis coupled with mass spectrometry or spectrofluorimetry.…”

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confidence: 99%

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High Affinity Aptamer for the Detection of the Biogenic Amine Histamine

et al. 2019

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The importance of histamine in various physiological functions and its involvement in allergenic responses make this small molecule one of the most studied biogenic amines. Even though a variety of chromatography-based methods have been described for its analytical determination, the disadvantages they present in terms of cost, analysis time, and low portability limit their suitability for in situ routine testing. In this work, we sought to identify histamine-binding aptamers that could then be exploited for the development of rapid, facile, and sensitive assays for histamine detection suitable for point-of-need analysis. A classic SELEX process was designed employing magnetic beads for target immobilization and the selection was completed after ten rounds. Following Next Generation Sequencing of the last selection rounds from both positive and counter selection magnetic beads, several sequences were identified and initially screened using an apta-PCR affinity assay (APAA). Structural and functional characterization of the candidates resulted in the identification of the H2 aptamer. The high binding affinity of the H2 aptamer to histamine was validated using four independent assays (K D of 3−34 nM). Finally, the H2 aptamer was used for the development of a magnetic beads-based competitive assay for the detection of histamine in both buffer and synthetic urine, achieving very low limits of detection of 18 pM and 76 pM, respectively, while no matrix effects were observed. These results highlight the suitability of the strategy followed for identifying small moleculebinding aptamers and the compatibility of the selected H2 aptamer with the analysis of biological samples, thus facilitating the development of point-of-care devices for routine testing. Ongoing work is focused on extending the application of the H2 aptamer to the detection of spoilage in meat, fish, and beverages, as well as evaluating the affinity of truncated forms of the aptamer.

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Section: ■ Results and Discussionmentioning

confidence: 99%

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confidence: 99%

High Affinity Aptamer for the Detection of the Biogenic Amine Histamine

et al. 2019

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“…1.7, 1.8 μm) will be, in our opinion, of a great interest future science group Quantitative analysis of drugs in biological matrices Review in bioanalytical applications. The implementation of this latter equipment has emerged in 2012 for quantitative assay of histamine [65,66] and lately for L-tetrahydropalmatine and cocaine in human plasma in 2014 [96]. Nowadays the new detectors are developed to cope this technology thanks to a cell of reduced volume (e.g.…”

Section: Future Perspectivementioning

confidence: 98%

“…The LOD was 2, 5 and 2 nM and the LOQ was 0.60, 0.60 and 0.32 ng/ml for glutathione, cysteine and N-acetylcysteine, respectively. In 2012, Guihen et al developed two UPLC methods to determine histamine in human urine [65] and psoriatic plaques [66]. Histamine is a heterocyclic primary amine extensively studied for its mediation of allergic response.…”

Section: Other Disordersmentioning

confidence: 99%

Quantitative Analysis of Drugs in Biological Matrices by HPLC Hyphenated to Fluorescence Detection

Lipka

Vaccher

2015

Bioanalysis

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An overview of the state-of-the art in HPLC coupled with fluorescence detection is presented. Over the last 20 years, the increasing number of methodological papers on this topic (4082 between 1994 and 2004 and 7725 between 2004 and 2014) is testament to its utility in bioanalytical applications. Compared with conventional UV absorbance detection used in HPLC, fluorescence detection can greatly enhance the sensitivity leading to limits of detection similar to those obtained with mass spectrometry, offering researchers a sensitive, robust and relatively inexpensive instrumental method. This work will focus on the analysis of pharmaceutical compounds in different biological matrices, either naturally fluorescent or derivatized with a fluorescent agent, and some of them chiral. Therapeutic applications, sample preparation and derivatization, sensitivity for each example are described.

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“…HA release could be measured in plasma, but also in other matrix such as urine [6], cerebrospinal fluid [7], or even cell culture supernatant [8]. Due to HA low concentrations in these different fluids even after an important release, sensitive quantification methods are needed.…”

Section: Introductionmentioning

confidence: 99%

Histamine quantification in human plasma using high resolution accurate mass LC–MS technology

Laurichesse

Gicquel

Moreau

et al. 2016

Clinical Biochemistry

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Tables, and 27 References A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPTABSTRACT Background: Histamine (HA) is a small amine playing an important role in anaphylactic reactions. In order to identify and quantify HA in plasma matrix, different methods have been developed but present several disadvantages. Here, we developed an alternative method using liquid chromatography coupled with an ultra-high resolution and accurate mass instrument, Q Exactive TM (Thermofisher) (LCHRMS). Methods:The method includes a protein precipitation of plasma samples spiked with HA-d4as internal standard (IS). LC separation was performed on a C18 Accucore column (100*2.1 mm, 2.6 μm) using a mobile phase containing nonafluoropentanoic acid (3 nM) and acetonitrile with 0.1% (v/v) formic acid on gradient mode. Separation of analytes was obtained within 10 min. Analysis was performed from full scan mode and targeted MS2 mode using a 5 ppm mass window. Ion transition monitored for targeted MS2 mode were 112.0869>95.0607m/z for HA and 116.1120>99.0855m/z for HAd4. Calibration curves were obtained by adding standard calibration dilution at 1 to 180 nM in TrisBSA.Results: Elution of HA and IS occurred at 4.1 min. The method was validated over a range of concentrations from 1 nM to 100 nM. The intra-and inter-run precisions were <15% for quality controls. Human plasma samples from 30 patients were analysed by LCHRMS, and the results were highly correlated with those obtained using the gold standard Radioimmunoassay (RIA) method. Conclusion:Overall, we demonstrate here that LCHRMS is a sensitive method for histamine quantification in biological human plasmas, suitable for routinely use in medical laboratories. In addition, LCHRMS is less time-consuming than RIA, avoid the use of radioactivity, and could then be considered as an alternative quantitative method.

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Histamine determination in human urine using sub‐2 μm C18 column with fluorescence and mass spectrometric detection

Cited by 13 publications

References 32 publications

High Affinity Aptamer for the Detection of the Biogenic Amine Histamine

High Affinity Aptamer for the Detection of the Biogenic Amine Histamine

Quantitative Analysis of Drugs in Biological Matrices by HPLC Hyphenated to Fluorescence Detection

Histamine quantification in human plasma using high resolution accurate mass LC–MS technology

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