Histamine Content and Histamine H Receptor in Experimentally-induced Nasal Hypersensitivity

Increased expression of histamine H1 receptor mRNA in allergic rhinitis

“…We demonstrated that the HiR mRNA is expressed in larger amount in nasal mucosa from the patients with allergic rhinitis than in that from the normal individuals. Kubo [12], Ishibe [13], and Shimada [14] have reported on the number of H, Rs by binding assay, but our report is on an overall expression of H, R mRNA by RT-PCR. It may be difficult to compare mRNA with a receptor protein and to make a Top.…”

“…It has been speculated that histamine hypersensensitivity of the nasal mucosa in allergic rhinitis is due to the enhanced affinity and/or density ofthe H, R on the sensory nerve and other effector cells and a few investigators have reported the quantitation of H|R [12][13][14]. To our knowledge, this study is the first report that specifically demonstrated the expression of H, R mRNA in the nasal scraping of both the patients with allergic rhinitis and the normal individuals.…”

“…It is reported that the minimum histamine concentration which induces sneezing is lower in allergic patients than in normal controls [10,1!]. In previous studies using binding assay, Kubo [12] and Ishibe [13] reported that there was no quantitative or qualitative difference in H|Rs between allergic nasal mucosa and that in normal, in guinea-pig and human nasal mucosa, respectively. On the other hand, Shimada [14] reported that the number of H,Rs was on the decrease with aging and it increased with the development of nasal allergy.…”

Effect of Glucocorticoid on Upregulation of Histamine H1Receptor mRNA in Nasal Mucosa of Rats Sensitized by Exposure to Toluene Diisocyanate

“…Shimada (8) also reported that H 1 R bindings in the nasal mucosa increased with the development of nasal allergy. However, it was previously demonstrated that there were no differences in the density of H 1 R between allergic and normal nasal mucosa in human and guinea pig models (14,15). These controversial results may be due to non-specific binding of mepyramine, which is a ligand in the H 1 R binding assay.…”

Histamine H1‐receptors in the nasal mucosa: a mystery solved?

“…In the nasal mucosa, histamine H preceptors can be localized to the vascular endothelium using autoradiographic techniques [1], Reports that numbers of histamine receptors increase with the development of nasal allergy and decrease with aging conflict with reports that there is no quantitative or qualitative diflerence in the H)-receptors in the inflamed nasal mucosa of subjects with allergic rhinitis and the nasal mucosa of normal subjects [2][3][4], In these studies, receptor binding assays were utilized and specific pH]-mepyramine (pH]-pyrilamine) binding sites were assumed to represent the H]receptors. In this issue of Clinical and Experimental Allergy, lriyoshi et al provide more definitive evidence of increased numbers of Hi-receptors in the nasal mucosa of subjects with allergic rhinitis symptoms, as determined by an increased expression of histamine H|receptor mRNA [5], This new information is of considerable interest.…”