Hispidin Isolated from Phellinus linteus Protects Against Hydrogen Peroxide–Induced Oxidative Stress in Pancreatic MIN6N β-Cells

Abstract

Reactive oxygen species (ROS) have been shown to cause DNA damage, protein denaturation, loss of antioxidative enzyme activity, and lipid peroxidation. Thus, ROS are associated with tissue damage and are considered to be prime contributing factors in inflammation, diabetes, aging, and cancer. In this study, we investigated whether or not hispidin protects pancreatic MIN6N β-cells from oxidative stress caused by hydrogen peroxide. Treatment of MIN6N β-cells with 0.5 mM hydrogen peroxide for 4 hours caused significant loss of cell viability and an increase in the number of apoptotic cells. However, pretreatment of MIN6N β-cells with hispidin for 24 hours reduced loss of cell viability and decreased the number of apoptotic cells. In addition, 70 μM hispidin significantly scavenged intracellular ROS and inhibited apoptosis and caspase-3 induced by hydrogen peroxide. Furthermore, the generation of thiobarbituric acid-reactive substances was inhibited in the presence of hispidin in a dose-dependent manner. Also, 70 μM hispidin significantly increased insulin secretion in hydrogen peroxide-treated MIN6N β-cells. These results suggest that hispidin may be effective for protecting MIN6N β-cells from ROS toxicity in diabetes.

Immunomodulatory Activity of Red Ginseng against Influenza A Virus Infection

“…To investigate the effect of RGE on oxidative stress, the fluorescent probe H 2 DCFDA was used to evaluate the level of intracellular ROS [18]. Briefly, after various treatments, cells were incubated with 5 μM H 2 DCFDA solution in PBS for 2 h at 37 °C.…”

β-Cell protection and antidiabetic activities of Crassocephalum crepidioides (Asteraceae) Benth. S. Moore extract against alloxan-induced oxidative stress via regulation of apoptosis and reactive oxygen species (ROS)

“…The alloxan-induced pancreatic β-cell damage through the generation of cytotoxic reactive oxygen species (ROS) [6–9]. The ROS produced by alloxan is responsible for the breakdown of DNA strand of pancreatic β–cells [10]. ROS induces oxidative stress that plays a pathological role in the development and progression of diabetic complications [11].…”

Anti‐inflammatory activity of mushroom‐derived hispidin through blocking of NF‐κB activation

“…Hispidin (Fig. A), a polyphenol compound mainly derived from a valuable medicinal mushroom, Phellinus linteus , has been found to possess strong antioxidant, anti‐cancer, and DNA damage protection activities . However, the anti‐inflammatory potential of hispidin is not yet well studied.…”