Highly Transparent Cyclic Olefin Copolymer Film with a Nanotextured Surface Prepared by One-Step Photografting for High-Density DNA Immobilization

Abstract

Compared with conventional glass slides and two-dimensional (2D) planar microarrays, polymer-based support materials and three-dimensional (3D) surface structures have attracted increasing attention in the field of biochips because of their good processability in microfabrication and low cost in mass production, as well as their improved sensitivity and specificity for the detection of biomolecules. In the present study, UV-induced emulsion graft polymerization was carried out on a cyclic olefin copolymer (COC) surface to generate 3D nanotextures composed of loosely stacked nanoparticles with a diameter of approximately 50 nm. The introduction of a hierarchical nanostructure on a COC surface only resulted in a 5% decrease in its transparency at a wavelength of 550 nm but significantly increased the surface area, which markedly improved immobilization density and efficiency of an oligonucleotide probe compared with the functional group and polymer brush-modified substrates. The highest immobilization efficiency of the probes reached 93%, and a limit of detection of 75 pM could be obtained. The hybridization experiment demonstrated that the 3D gene chip exhibited excellent sensitivity for target DNA detection and single-nucleotide polymorphism discrimination. This one-step approach to the construction of nanotextured surfaces on the COC has promising applications in the fields of biochips and immunoassays.

UV-Assisted Li+-Catalyzed Radical Grafting Polymerization of Vinyl Ethers: A New Strategy for Creating Hydrolysis-Resistant and Long-Lived Polymer Brushes as a “Smart” Surface Coating

“…The immobilization efficiency was quantified by evaluating the fluorescence intensity of the protein region before and after the final washing step. However, the immobilization efficiency was calculated to be only 20%, much lower than the value reported in our previous work, and it was mainly associated with the amount of −OH group and its reactivity, speculatively. On the whole, the PVE antifouling layer showed excellent anti-nonspecific protein interference performance, and it significantly increased the amount of chemically immobilized antibodies on the surface.…”

Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry

“…hybridization density = 0.43 pmol cm −2 ). 33 In agreement with the lower amount of surface reactive groups on S-azide slides, the hybridization density of S-azide-P-DNA surfaces culminated at 1.5 pmol cm −2 (entry 12).…”

One-step fabrication of three-dimensional macropore copolymer-modified polycarbonate array by photo-crosslinking for protein immunoassay

“…Due to the porous structure of the MC-PC surface, the hydrophilicity and hydrophobicity of the structure have an important influence on the penetration efficiency of the aqueous protein solution. 49 The water contact angle (CA) of the pristine PC surface is 78°, and the CA value of the PC surface modified by copolymer decreases (Fig. S8 † ).…”