Highly specific C–C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging

Abstract

A novel FRET fluorescence “off–on” system based on the highly specific, sensitive and effective C–C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO).

An N-nitrosation reactivity-based two-photon fluorescent probe for the specific in situ detection of nitric oxide

“… 6 Furthermore, the OPD moiety is also easily oxidized by some ROS and RNS, such as H 2 O 2 and ONOO – . 7 This poor selectivity has provoked broad concern about the OPD-dependent design of the NO probe. Therefore, it is still highly demanded and challenging to develop a fluorescent probe for NO with the desired selectivity.…”

In Vivo Biosensing Using Resonance Energy Transfer

“…Three days after a subcutaneous injection of Freund’s adjuvant to initiate inflammation on the left rear paws of mice, 0.5 mg kg −1 DHPFQ was injected intravenously and the mice imaged every ten minutes for one hour (Figure 6A). Using the semi-quantitative analysis of a region of interest (ROI) on the fluorescence image, their findings showed an 8-fold increase in fluorescence intensity of FITC within 10 min post-injection in the inflamed region of the left paw as compared to the non-inflamed paw, leading to nanomolar detection of NO (Figure 6B) [114].…”

“…Fluorescence signals were collected at 600 nm after 470 nm excitation. Reprinted from [114]. Distributed under a Creative Commons Attribution 3.0 Unported License (CC BY 3.0).…”

2-Amino-3′-dialkylaminobiphenyl-based fluorescent intracellular probes for nitric oxide surrogate N2O3

“…[6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] In order to establish NO levels and activity, scientists have employed NO-specic electrodes [26][27][28][29][30][31][32][33][34] and chemical probes that, upon reaction with NO or its surrogates, produce a change in electron paramagnetic resonance (EPR), [35][36][37][38][39] UV-vis absorbance, 40,41 chemiluminescence, [42][43][44][45] and/or uorescence. [46][47][48][49][50][51][52][53][54][55]…”