Highly sensitive visual colorimetric sensor for xanthine oxidase detection by using MnO2-nanosheet-modified gold nanoparticles

Lian, Yawen; Xiang, Yuan; Wang, Yandan; Wei, Lin

doi:10.1016/j.saa.2022.121219

Cited by 8 publications

(1 citation statement)

References 38 publications

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“…To date, dark-field microscopy (DFM) has developed as a promising optical tool in the field of chemical reaction monitoring, − real-time optical sensing, − metal ions’ quantitative analysis, , and live cell imaging at single-cell level. − Compared with fluorescence microscopy imaging, DFM possesses the imaging characteristic of extraordinary contrast between the bright light scattering probes and the dark background, which overcome the inherent disadvantages of fluorescent molecules such as photobleaching and photo-damage . Meanwhile, every individual nanoprobe under DFM could act as an independent probe, which offers lower detection limits and higher spatial resolution than other methods based on ensemble analysis. − For instance, the Xu group detected microRNA in living cells with gold nanoparticles (Au NPs). , Kang and his co-workers realized the imaging of sub-10 nm Au NPs within a cellular medium under absorption modulated scattering microscopy .…”

Section: Introductionmentioning

confidence: 99%

Dark-Field Imaging Monitoring of Adenosine Triphosphate in Live Cells by Au NBPs@ZIF-8 Nanoprobes

et al. 2022

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Monitoring the fluctuation of adenosine triphosphate (ATP) level in living cells could promote the understanding of metabolic pathways and cell biology. Here, we proposed a highly sensitive, selective, and biocompatible nanoprobe with core−shell structure, namely Au NBPs@ZIF-8 composed by gold nanobipyramids (Au NBPs) and zeolitic imidazolate framework-8 (ZIF-8), for monitoring intracellular ATP level fluctuation in living cells. Because the coordination between ATP and Zn 2+ (the metal node of ZIF-8) was much stronger than that between 2-methylimidazole and Zn 2+ , which caused the decomposition of the ZIF-8 shell and the exposure of Au NBPs in the presence of ATP, it led to the change of the localized surface plasmon resonance scattering properties of nanoprobes under dark-field microscopy. Tricolor (RGB) analysis showed that R/G value had a good linear relationship with the ATP concentrations in the range of 10 μM to 4 mM (R 2 = 0.999) with a detection limit of 5.28 μM. This ATP sensing platform also exhibited excellent selectivity in complex intracellular interfering substances. Besides, we realized intracellular ATP real-time imaging in HeLa cells and observed the ATP level fluctuation under dark-field microscopy. The method mentioned here could be further applied for delivery of therapeutics for biomedical applications.

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