Highly Sensitive Virome Capture Sequencing Technique VirCapSeq-VERT Identifies Partial Noncoding Sequences but no Active Viral Infection in Cutaneous T-Cell Lymphoma

Cutaneous T-Cell Lymphoma Skin Microbiome Is Characterized by Shifts in Certain Commensal Bacteria but not Viruses when Compared with Healthy Controls

“…While the biology of these malignancies is not fully understood, some microbes, particularly viruses, have been hypothesized to play roles in malignant T cell transformation in CTCL (Berger et al, 2002, Mirvish et al, 2013, van der Loo et al, 1979. However, high throughput sequencing approaches have failed to consistently detect viral sequences in the skin or peripheral blood of CTCL patients (Anderson et al, 2018, Dereure et al, 2013. Infections are common in advanced stage patients, and antibiotic treatment results in skin improvement and decreased disease activity (Lindahl et al, 2019).…”

Mycosis Fungoides and Sézary Syndrome

“…45,46 Recently, a more sophisticated genomic analysis of SS samples using VirCapSeq-VERT revealed no functional coding sequences for viral pathogens or unknown viruses or evidence for active infection. 47 Interestingly, partially coding proviral sequences of human endogenous retroviruses (HERVs) were detected. 47 Although these particles may play a pathogenic role in disease, given that HERVs are normally present in the human genome, demonstrating a causal role in CTCL will be challenging.…”

“…47 Interestingly, partially coding proviral sequences of human endogenous retroviruses (HERVs) were detected. 47 Although these particles may play a pathogenic role in disease, given that HERVs are normally present in the human genome, demonstrating a causal role in CTCL will be challenging. 48…”