Highly Sensitive Urine‐Based Enzyme‐Linked Immunosorbent Assay for Detection of Antibody to <i>Helicobacter pylori</i>

Katsuragi, Kiyonori; Noda, Atsunari; Tachikawa, Tetsuya; Azuma, Atsushi; Mukai, Fumie; Murakami, Kazunari; Fujioka, Toshio; Kato, Mototsugu; Asaka, Masahiro

doi:10.1046/j.1523-5378.1998.08045.x

Cited by 61 publications

(90 citation statements)

References 19 publications

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“…The advantages of urine-based enzyme immunoassays are that they are noninvasive, the sample collection is very simple (3,6,7), it does not require centrifugation, and the cost of using urine as a sample is much lower than that of serum (4,7,20). Antibody concentration in urine samples was approximately 1/10000 of antibody concentration in serum (12,21). Although anti-H. pylori IgG is at a low concentration in urine samples, it has been found to correlate with anti-H. pylori IgG in serum samples (5,20).…”

Section: Discussionmentioning

confidence: 96%

“…The performance of an antibody detection-based kit is affected by the ethnic factor, and therefore, it is different in various patient populations (26). URINELISA assay plates and RAPIRUN membranes contain antigens from H. pylori strain (OHPC-040), which have important genes (vacA, ureB and cagA) from Japanese patients (3,9,11,18,21,22). However, the urine antibody test accuracy did not differ between Asian and Western ethnic groups (26).…”

Section: Discussionmentioning

confidence: 99%

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The diagnostic accuracy of urine IgG antibody tests for the detection of Helicobacter pylori infection in Turkish dyspeptic patients

Gürbüz

Gönen²,

Bekmen³

et al. 2012

Turk J Gastroenterol

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Girifl ve Amaç: Helikobakter pilori enfeksiyonunun de¤erlendirilmesinde invaziv olmayan testlere ilgi artm›fl ve idrar temelli testler Helikobakter pilori enfeksiyonunu saptamada invaziv olmayan testler olarak s›k kullan›lmaktad›r. Bu çal›flmada Türk eriflkin dispeptik hastalarda idrarda sal›nan anti-Helikobakter pilori IgG antikorunu saptamada hasta bafl› idrar kart testi ve idrar antikor enzim-ba¤l›

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

The diagnostic accuracy of urine IgG antibody tests for the detection of Helicobacter pylori infection in Turkish dyspeptic patients

Gürbüz

Gönen²,

Bekmen³

et al. 2012

Turk J Gastroenterol

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“…21,22 The high sensitivity of the test may be explained in part by the profiles of antigens extracted from the whole cell of H pylori. 20 Moreover, the addition of extracted proteins of E coli to the assay system reduces cross reactivity, probably contributing to the high degree of specificity. In this study, the specificity of the urine-based ELISA was consistently high, ranging from 93% to 99%.…”

Section: Discussionmentioning

confidence: 99%

“…H pylori-specific IgG antibodies in urine are stable for at least 60 days at 4°C. 20 Therefore, the method should be suitable for large-scale epidemiologic studies concerning the organism. In the near future, the urine-based ELISA method might be available for noninvasive diagnosis of various infectious diseases.…”

Section: Discussionmentioning

confidence: 99%

“…An OHPC-040 H pylori strain (vacA and cagA gene-positive) isolated from a Japanese patient with gastritis was chosen as the best strain for the antigen of the urine-based ELISA. 20 On H pylori extracted antigen-coated 96-well microtiter plate, 100 L of urine samples, 2 positive controls, and 3 negative controls were incubated in 0.2 M Tris aminomethane chloride buffer (pH 7.3) containing 0.001% Escherichia coli extracted proteins at 37°C for 1 hour. After washing the wells with buffer solution, 100 L of horseradish peroxidaseconjugated anti-human goat IgG antibody was added and incubated at 37°C for 1 hour.…”

Section: Urine-based Elisa Methodsmentioning

confidence: 99%

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Urine-Based Enzyme-Linked Immunosorbent Assay for the Detection of Helicobacter pylori Infection in Children

et al. 2001

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ABSTRACT. Objective. Serology and 13 C-urea breath test have been widely used as noninvasive tests to detect Helicobacter pylori infection. However, easier collection of samples and lower costs are desirable for diagnosis of the individual patient or for use in epidemiologic studies. Our aim was to study the diagnostic accuracy of a recently developed urine-based enzyme-linked immunosorbent assay (ELISA) kit for the detection of H pylorispecific immunoglobulin G (IgG) antibodies in children.Study Design. Specimens of serum and randomly voided urine were collected from 816 children (0 -15 years old) and were analyzed using 2 serum-based ELISA kits and a urine-based ELISA kit, respectively. Based on results of serology, the sensitivity, specificity, and accuracy of the urine-based ELISA kit were evaluated. With regard to false-positive and false-negative results, urinary IgG concentrations and IgG/creatinine levels were studied.Results. Both serum-based ELISAs were positive in 41 children and were negative in 666, who were enrolled in this study. The remaining 109 children were excluded because of disagreement between the results of the 2 serum-based ELISAs, including indeterminate values. Overall sensitivity, specificity, and accuracy of urinebased ELISA test compared with serology were 85.4%, 95.5%, and 94.9%, respectively. On positivity rates, the urine-based ELISA was closely coincident with the serum-based ELISA in each age group. There was no correlation between antibody levels detected by urine-based ELISA and each serum-based ELISA. Urinary IgG concentrations and IgG/creatinine levels were significantly higher in false-positives and were lower in false-negatives than in true-positives plus true-negatives for serology. Most of those with false-positive results had trace to moderate proteinuria.Conclusions. The urine-based ELISA is an alternative to serum-based ELISA for diagnosis of H pylori infection in children and should be suitable for large-scale epidemiologic studies concerning the organism. In children with proteinuria, results of the test should be interpreted with caution. It is possible that the urine-based ELISA method would be applicable to diagnosis of other infectious diseases. Pediatrics 2001;107(6). URL: http://www. pediatrics.org/cgi/content/full/107/6/e87; enzyme-linked immunosorbent assay, Helicobacter pylori, immunoglobulin G, urine.

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Non-invasive diagnostic tests forHelicobacter pyloriinfection

Best

Takwoingi

Siddique

et al. 2018

Cochrane Database of Systematic Reviews

154

130

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In people without a history of gastrectomy and those who have not recently had antibiotics or proton ,pump inhibitors, urea breath tests had high diagnostic accuracy while serology and stool antigen tests were less accurate for diagnosis of Helicobacter pylori infection.This is based on an indirect test comparison (with potential for bias due to confounding), as evidence from direct comparisons was limited or unavailable. The thresholds used for these tests were highly variable and we were unable to identify specific thresholds that might be useful in clinical practice.We need further comparative studies of high methodological quality to obtain more reliable evidence of relative accuracy between the tests. Such studies should be conducted prospectively in a representative spectrum of participants and clearly reported to ensure low risk of bias. Most importantly, studies should prespecify and clearly report thresholds used, and should avoid inappropriate exclusions.

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Highly Sensitive Urine‐Based Enzyme‐Linked Immunosorbent Assay for Detection of Antibody to Helicobacter pylori

Cited by 61 publications

References 19 publications

The diagnostic accuracy of urine IgG antibody tests for the detection of Helicobacter pylori infection in Turkish dyspeptic patients

The diagnostic accuracy of urine IgG antibody tests for the detection of Helicobacter pylori infection in Turkish dyspeptic patients

Urine-Based Enzyme-Linked Immunosorbent Assay for the Detection of Helicobacter pylori Infection in Children

Non-invasive diagnostic tests forHelicobacter pyloriinfection

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