Highly sensitive transient absorption imaging of graphene and graphene oxide in living cells and circulating blood

Li, Junjie; Zhang, Weixia; Chung, Ting-Fung; Slipchenko, Mikhail N.; Chen, Yong P.; Cheng, Ji‐Xin; Yang, Chen

doi:10.1038/srep12394

Cited by 30 publications

(22 citation statements)

References 60 publications

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“…Moreover, transient absorption imaging can also be realized through exogenous contrast agents such as nanoparticles. The non-fluorescent contrasts have been generated from several nanostructures in biological samples, including semiconducting and metallic carbon nanotubes 241 , nanodiamonds 242 , gold nanorods 243 and graphene/graphene oxide 244 .…”

Section: Pump-probe Transient Absorptionmentioning

confidence: 99%

Recent advances in nonlinear optics for bio-imaging applications

Zhang

Liu²,

Ren

et al. 2020

Opto-Electronic Advances

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Nonlinear optics, which is a subject for studying the interaction between intense light and materials, has great impact on various research fields. Since many structures in biological tissues exhibit strong nonlinear optical effects, nonlinear optics has been widely applied in biomedical studies. Especially in the aspect of bio-imaging, nonlinear optical techniques can provide rapid, label-free and chemically specific imaging of biological samples, which enable the investigation of biological processes and analysis of samples beyond other microscopy techniques. In this review, we focus on the introduction of nonlinear optical processes and their applications in bio-imaging as well as the recent advances in this filed. Our perspective of this field is also presented.

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Section: Pump-probe Transient Absorptionmentioning

confidence: 99%

Recent advances in nonlinear optics for bio-imaging applications

Zhang

Liu²,

Ren

et al. 2020

Opto-Electronic Advances

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“…Transient absorption imaging has been used to generate non-fluorescent contrast in biological tissue from several types of nanostructures, including semiconducting and metallic single-walled carbon nanotubes, 77,171 nanodiamonds, 85 gold nanorods, 86 and graphene and graphene oxide. 172…”

Section: B Biological Imagingmentioning

confidence: 99%

Invited Review Article: Pump-probe microscopy

Fischer

Wilson

Robles

et al. 2016

Review of Scientific Instruments

202

143

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Multiphoton microscopy has rapidly gained popularity in biomedical imaging and materials science because of its ability to provide three-dimensional images at high spatial and temporal resolution even in optically scattering environments. Currently the majority of commercial and home-built devices are based on two-photon fluorescence and harmonic generation contrast. These two contrast mechanisms are relatively easy to measure but can access only a limited range of endogenous targets. Recent developments in fast laser pulse generation, pulse shaping, and detection technology have made accessible a wide range of optical contrasts that utilize multiple pulses of different colors. Molecular excitation with multiple pulses offers a large number of adjustable parameters. For example, in two-pulse pump-probe microscopy, one can vary the wavelength of each excitation pulse, the detection wavelength, the timing between the excitation pulses, and the detection gating window after excitation. Such a large parameter space can provide much greater molecular specificity than existing single-color techniques and allow for structural and functional imaging without the need for exogenous dyes and labels, which might interfere with the system under study. In this review, we provide a tutorial overview, covering principles of pump-probe microscopy and experimental setup, challenges associated with signal detection and data processing, and an overview of applications.

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“…In recent years, the commercial use of nanomaterials has been significantly increased especially in medical applications 14 , 15 . Carbon nanomaterials with unique structural and physical properties have gained a great deal of attention in a large range of biomedical fields such as cellular imaging and drug delivery 16 , 17 .…”

Section: Introductionmentioning

confidence: 99%

Microfluidic investigation of the effect of graphene oxide on mechanical properties of cell and actin cytoskeleton networks: experimental and theoretical approaches

Ghorbani

Soleymani

Hashemzadeh

et al. 2021

Sci Rep

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Biomechanical and morphological analysis of the cells is a novel approach for monitoring the environmental features, drugs, and toxic compounds’ effects on cells. Graphene oxide (GO) has a broad range of medical applications such as tissue engineering and drug delivery. However, the effects of GO nanosheets on biological systems have not been completely understood. In this study, we focused on the biophysical characteristics of cells and their changes resulting from the effect of GO nanosheets. The biophysical properties of the cell population were characterized as follows: cell stiffness was calculated by atomic force microscopy, cell motility and invasive properties were characterized in the microfluidic chip in which the cells are able to visualize cell migration at a single-cell level. Intracellular actin was stained to establish a quantitative picture of the intracellular cytoskeleton. In addition, to understand the molecular interaction of GO nanosheets and actin filaments, coarse-grained (CG) molecular dynamics (MD) simulations were carried out. Our results showed that GO nanosheets can reduce cell stiffness in MCF7 cells and MDA-MB-231 cell lines and highly inhibited cell migration (39.2%) in MCF-7 and (38.6%) in MDA-MB-231 cell lines through the GO nanosheets-mediated disruption of the intracellular cytoskeleton. In the presence of GO nanosheets, the cell migration of both cell lines, as well as the cell stiffness, significantly decreased. Moreover, after GO nanosheets treatment, the cell actin network dramatically changed. The experimental and theoretical approaches established a quantitative picture of changes in these networks. Our results showed the reduction of the order parameter in actin filaments was 23% in the MCF7 cell line and 20.4% in the MDA-MB-231 cell line. The theoretical studies also showed that the GO nanosheet–actin filaments have stable interaction during MD simulation. Moreover, the 2D free energy plot indicated the GO nanosheet can induce conformational changes in actin filaments. Our findings showed that the GO nanosheets can increase the distance of actin-actin subunits from 3.22 to 3.5 nm and in addition disrupt native contacts between two subunits which lead to separate actin subunits from each other in actin filaments. In this study, the biomechanical characteristics were used to explain the effect of GO nanosheets on cells which presents a novel view of how GO nanosheets can affect the biological properties of cells without cell death. These findings have the potential to be applied in different biomedical applications.

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Highly sensitive transient absorption imaging of graphene and graphene oxide in living cells and circulating blood

Cited by 30 publications

References 60 publications

Recent advances in nonlinear optics for bio-imaging applications

Recent advances in nonlinear optics for bio-imaging applications

Invited Review Article: Pump-probe microscopy

Microfluidic investigation of the effect of graphene oxide on mechanical properties of cell and actin cytoskeleton networks: experimental and theoretical approaches

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