Highly sensitive, stable and selective hydrogen peroxide amperometric biosensors based on peroxidases from different sources wired by Os-polymer: A comparative study

Bollella, Paolo; Medici, Luca; Tessema, Merid; Полозников, А. А.; Hushpulian, Dmitry M.; Тишков, В. И.; Andreu, Rafael; Leech, Dónal; Megersa, Negussie; Marcaccio, Massimo; Gorton, Lo; Antiochia, Riccarda

doi:10.1016/j.ssi.2017.10.015

Cited by 25 publications

(19 citation statements)

References 87 publications

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“…In this case, it is likely that the H 2 O 2 detection follows an alternative non-native route, which proceeds via formation of the ferrous [Fe(II)] enzyme. This low potential redox cycle of peroxidase electrocatalysis has been associated with non-native enzyme states and transient formation of inactive catalytic intermediates (e.g., Fe(II)-O 2 ), which can also be generated in the presence of high concentrations of H 2 O 2 [51,91,95,96]. Among the DET mechanisms proposed to rationalize these experimental findings (cf.…”

Section: Detection Modes In Peroxidase-based Biosensorsmentioning

confidence: 99%

Electrocatalysis by Heme Enzymes—Applications in Biosensing

et al. 2021

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Heme proteins take part in a number of fundamental biological processes, including oxygen transport and storage, electron transfer, catalysis and signal transduction. The redox chemistry of the heme iron and the biochemical diversity of heme proteins have led to the development of a plethora of biotechnological applications. This work focuses on biosensing devices based on heme proteins, in which they are electronically coupled to an electrode and their activity is determined through the measurement of catalytic currents in the presence of substrate, i.e., the target analyte of the biosensor. After an overview of the main concepts of amperometric biosensors, we address transduction schemes, protein immobilization strategies, and the performance of devices that explore reactions of heme biocatalysts, including peroxidase, cytochrome P450, catalase, nitrite reductase, cytochrome c oxidase, cytochrome c and derived microperoxidases, hemoglobin, and myoglobin. We further discuss how structural information about immobilized heme proteins can lead to rational design of biosensing devices, ensuring insights into their efficiency and long-term stability.

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Section: Detection Modes In Peroxidase-based Biosensorsmentioning

confidence: 99%

Electrocatalysis by Heme Enzymes—Applications in Biosensing

et al. 2021

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“…Bollella et at. presented an interesting comparison between the use of two different peroxidases enzymes to make a hydrogen peroxide biosensor ( Figure 14 ), the cationic horseradish peroxidase (HRP), and the anionic tobacco peroxidase (TOP), combined with a highly cationic osmium polymer [Os(4,4

-dimethyl-2,2

-bipyridine) 2poly(N-vinylimidazole)10Cl]

([Os(dmp)PVI]

) [ 110 ]. They immobilized both enzymes separately onto graphite rod (G) electrodes through a three-step drop-casting procedure, to make two biosensors, one named as HRP/PEGDGE/[Os(dmp)PVI]

/G and the other as TOP/PEGDGE/[Os(dmp)PVI]

/G electrodes, with each enzyme, respectively.…”

Section: Materials Used For Electrocatalytic Hydrogen Peroxide Senmentioning

confidence: 99%

Nanostructures in Hydrogen Peroxide Sensing

Trujillo

Barraza

Zamora

et al. 2021

Sensors

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In recent years, several devices have been developed for the direct measurement of hydrogen peroxide (), a key compound in biological processes and an important chemical reagent in industrial applications. Classical enzymatic biosensors for have been recently outclassed by electrochemical sensors that take advantage of material properties in the nano range. Electrodes with metal nanoparticles (NPs) such as Pt, Au, Pd and Ag have been widely used, often in combination with organic and inorganic molecules to improve the sensing capabilities. In this review, we present an overview of nanomaterials, molecules, polymers, and transduction methods used in the optimization of electrochemical sensors for sensing. The different devices are compared on the basis of the sensitivity values, the limit of detection (LOD) and the linear range of application reported in the literature. The review aims to provide an overview of the advantages associated with different nanostructures to assess which one best suits a target application.

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“…The heme peroxidase from horseradish (HRP) is probably the best-studied member of this family and therefore, the most popular peroxidase for the construction of electrochemical biosensors. However, systems employing other heme peroxidases e.g., from soybean [ 25 , 26 , 27 ], tobacco [ 28 , 29 , 30 ], as well as peanut and sweet potato [ 28 , 31 ], have been reported.…”

Section: Heme Peroxidases and Their Mimicsmentioning

confidence: 99%

Electrochemical Biosensors Employing Natural and Artificial Heme Peroxidases on Semiconductors

Neumann

Wollenberger

2020

Sensors

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Heme peroxidases are widely used as biological recognition elements in electrochemical biosensors for hydrogen peroxide and phenolic compounds. Various nature-derived and fully synthetic heme peroxidase mimics have been designed and their potential for replacing the natural enzymes in biosensors has been investigated. The use of semiconducting materials as transducers can thereby offer new opportunities with respect to catalyst immobilization, reaction stimulation, or read-out. This review focuses on approaches for the construction of electrochemical biosensors employing natural heme peroxidases as well as various mimics immobilized on semiconducting electrode surfaces. It will outline important advances made so far as well as the novel applications resulting thereof.

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Highly sensitive, stable and selective hydrogen peroxide amperometric biosensors based on peroxidases from different sources wired by Os-polymer: A comparative study

Cited by 25 publications

References 87 publications

Electrocatalysis by Heme Enzymes—Applications in Biosensing

Electrocatalysis by Heme Enzymes—Applications in Biosensing

Nanostructures in Hydrogen Peroxide Sensing

Electrochemical Biosensors Employing Natural and Artificial Heme Peroxidases on Semiconductors

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