Highly sensitive smartphone-based detection of Listeria monocytogenes using SYTO9

Liu, Sha; He, Xuliang; Zhang, Tao; Zhao, Kaihui; Xiao, Changhu; Tong, Zengrui; Jin, Lian; He, Nongyue; Deng, Yan; Song, Li; Guo, Yuan; Chen, Zhu

doi:10.1016/j.cclet.2021.11.051

Cited by 31 publications

(18 citation statements)

References 33 publications

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“…In addition to replication activity, polymerases with high chain displacement activity are required for amplification ( Garafutdinov et al, 2020 ; Padzil et al, 2022 ). Liu et al (2022) developed a LAMP method for LM detection using SYTO9 staining and image processing techniques. The detection limit of LM was 6 copies/μl.…”

Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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The global burden of foodborne disease is enormous and foodborne pathogens are the leading cause of human illnesses. The detection of foodborne pathogenic bacteria has become a research hotspot in recent years. Rapid detection methods based on immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry have developed rapidly and become the main methods for the detection of foodborne pathogens. This study reviewed a variety of rapid detection methods in recent years. The research advances are introduced based on the above technical methods for the rapid detection of foodborne pathogenic bacteria. The study also discusses the limitations of existing methods and their advantages and future development direction, to form an overall understanding of the detection methods, and for point-of-care testing (POCT) applications to accurately and rapidly diagnose and control diseases.

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Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

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“…Vision-based biosensors often do not require large instruments and equipment [ 196 , 197 ], and a small microscope [ 198 , 199 ] or a smartphone [ 200 , 201 ] can read out the detection signal through naked-eye observations [ 202 , 203 , 204 ]. Nano- or micromotors are inspired by natural movements, such as those of sperm, bacteria, or molecular motors, and are promising materials in many biomedical applications [ 205 ].…”

Section: Janus Particles For Optical Poctmentioning

confidence: 99%

Application of Janus Particles in Point-of-Care Testing

et al. 2022

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Janus particles (JPs), named after the two-faced Roman god, are asymmetric particles with different chemical properties or polarities. JPs have been widely used in the biomedical field in recent years, including as drug carriers for targeted controlled drug release and as biosensors for biological imaging and biomarker detection, which is crucial in the early detection and treatment of diseases. In this review, we highlight the most recent advancements made with regard to Janus particles in point-of-care testing (POCT). Firstly, we introduce several commonly used methods for preparing Janus particles. Secondly, we present biomarker detection using JPs based on various detection methods to achieve the goal of POCT. Finally, we discuss the challenges and opportunities for developing Janus particles in POCT. This review will facilitate the development of POCT biosensing devices based on the unique properties of Janus particles.

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“…The targets of biosensors also include the pathogens that cause diseases in humans, such as cancerous cells, harmful bacteria, and viruses [ 127 , 128 , 129 , 130 , 131 ]. The main method of detecting cells and bacteria was achieved by the interaction between cell membranes and compounds that have a strong affinity for cells and bacteria [ 132 , 133 ]. Moreover, monitoring the role of substances in living cells is important for understanding the behavior and heterogeneity of tumor cells [ 134 ].…”

Section: Overview Of Lc Droplet-based Biosensors For Poc Diagnosis Of...mentioning

confidence: 99%

Liquid Crystal Droplet-Based Biosensors: Promising for Point-of-Care Testing

Xie

et al. 2022

Biosensors

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The development of biosensing platforms has been impressively accelerated by advancements in liquid crystal (LC) technology. High response rate, easy operation, and good stability of the LC droplet-based biosensors are all benefits of the long-range order of LC molecules. Bioprobes emerged when LC droplets were combined with biotechnology, and these bioprobes are used extensively for disease diagnosis, food safety, and environmental monitoring. The LC droplet biosensors have high sensitivity and excellent selectivity, making them an attractive tool for the label-free, economical, and real-time detection of different targets. Portable devices work well as the accessory kits for LC droplet-based biosensors to make them easier to use by anyone for on-site monitoring of targets. Herein, we offer a review of the latest developments in the design of LC droplet-based biosensors for qualitative target monitoring and quantitative target analysis.

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Highly sensitive smartphone-based detection of Listeria monocytogenes using SYTO9

Cited by 31 publications

References 33 publications

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Application of Janus Particles in Point-of-Care Testing

Liquid Crystal Droplet-Based Biosensors: Promising for Point-of-Care Testing

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