Highly sensitive sequencing reveals dynamic modifications and activities of small RNAs in mouse oocytes and early embryos

Yang, Qiyuan; Lin, Jimin; Liu, Miao; Li, Ronghong; Tian, Bin; Zhang, Xue; Xu, Beiying; Liu, Mofang; Zhang, Xuan; Li, Yiping; Shi, Huijuan; Wu, Ligang

doi:10.1126/sciadv.1501482

Cited by 110 publications

(115 citation statements)

References 75 publications

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“…They were first identified in humans (Ostermeier et al 2005), followed by confirmation of their presence in mice (Amanai et al 2006) and have now been detected in various mammals, including porcine (Curry et al 2011, Kasimanickam & Kastelic 2016, bulls (Stowe et al 2014), stallions (Das et al 2013), rats and rabbits . It has been demonstrated that mature miRNAs that are carried into the zygote by sperm during the fertilization are relatively rare compared with oocyte miRNAs (Yang et al 2016). Accumulating evidence shows that miRNAs are critical for fertilization and early embryonic development (Liu et al 2012a,b, Cheong et al 2014.…”

Section: Introductionmentioning

confidence: 99%

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

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The similarities and differences of small RNAs in seminal plasma, epididymal sperm and ejaculated sperm remain largely undefined. We conducted a systematic comparative analysis of small RNA profiles in pig ejaculated sperm, epididymal sperm and seminal plasma and found that the diversity distribution of small RNA species was generally similar, whereas the abundance of small RNAs is dramatically different across the three libraries; miRNAs and small RNAs derived from rRNA, tRNA, small nuclear RNA, 7SK RNA, NRON RNA and cis-regulatory RNA were enriched in the three libraries, but piRNA was absent. A large population of small RNAs from ejaculated sperm are ejaculated sperm specific, and only 8-30% of small RNAs overlapped with those of epididymal sperm or seminal plasma and a small proportion (5-18%) of small RNAs were shared in the three libraries, suggesting that, in addition to the testes, sperm RNAs may also originate from seminal plasma, epididymis as well as other resources. Most miRNAs were co-distributed but differentially expressed across the three libraries, with epididymal sperm exhibiting the highest abundance, followed by ejaculated sperm and seminal plasma. The prediction of target genes of the top 10 highly expressed miRNAs across the three libraries revealed that these miRNAs may be involved in spermatogenesis, zygote development and the interaction between the environment and animals. Our study provides the first description of the similarities and differences of small RNA profiles in ejaculated sperm, epididymal sperm and seminal plasma and indicates that sperm RNA may have origins other than the testes.Reproduction (2017) 153 [785][786][787][788][789][790][791][792][793][794][795][796]

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Section: Introductionmentioning

confidence: 99%

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

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show abstract

“…This indicates a relatively lower expression of miRNAs during oocyte development. Consistent with our results, mouse oocytes showed the same pattern of a lower proportion of miRNAs with a rapid increase during early embryonic development (Yang et al, ). Ohnishi et al () reported that a transition of a major small RNA class from siRNA/piRNA to miRNA takes place during early preimplantation mouse embryos.…”

Section: Discussionmentioning

confidence: 99%

“…Kim et al, ). Using highly sensitive sequencing technology, Q. Yang et al () reported that let‐7 family miRNAs were among the most abundant miRNAs in mouse oocytes and early embryos, indicating their potential role in regulating early embryonic development. Moreover, miR‐16 and let‐7 family miRNAs were detected with a high expression level in mouse GV oocytes with an indication of their regulatory role during follicular development (Murchison et al, ).…”

Section: Discussionmentioning

confidence: 99%

microRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles

Gad

Němcová

Murín

et al. 2019

Molecular Reproduction Devel

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Oocyte developmental competence is acquired during folliculogenesis and regulated by complex molecular mechanisms. Several molecules are involved in these mechanisms, including microRNAs (miRNAs) that are essential for oocyte‐specific processes throughout the development. The objective of this study was to identify the expression profile of miRNAs in porcine oocytes derived from follicles of different sizes using RNA deep sequencing. Oocytes were aspirated from large (LO; 3–6 mm) or small (SO; 1.5–1.9 mm) follicles and tested for developmental competence and chromatin configurations. Small RNA libraries were constructed from both groups and then sequenced in an Illumina NextSeq. 500. Oocytes from the LO group exhibited higher developmental competence and different chromatin configuration compared with oocytes from the SO group. In total, 167 and 162 known miRNAs were detected in the LO and SO groups, respectively. MiR‐205, miR‐16, miR‐148a‐3p, and miR‐125b were among the top 10 highly expressed miRNAs in both groups. Eight miRNAs were differentially expressed (DE) between both groups. Target gene prediction and pathway analysis revealed 46 pathways that were enriched with miRNA‐target genes. The oocyte meiosis pathway and signaling pathways including FoxO, PI3K‐Akt, and cAMP were predictably targeted by DE miRNAs. These results give more insights into the potential role of miRNAs in regulating the oocyte development.

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“…However, injection of miR‐dKO round spermatids into fertile mouse oocyte successfully gave rise to healthy offspring (Yuan et al, ): indicating that this miRNA family is essential for normal spermatogenesis, but not for fertilization and embryonic development (Liu et al, ). Sperm‐derived miR‐191, miR‐16, and miR‐146a are the most abundant miRNAs in early zygotes of mice (Yang et al, ), but their roles are not clear. Despite being silenced functionally, the biogenesis of miRNAs is not abrogated during fertilization to ZGA in mice (Tang et al, ; Suh et al, ), suggesting that miRNAs might have important roles during transformation of zygotes to pluripotent blastocysts (Tang et al, ; Suh et al, ).…”

Section: Mirnas As Regulators Of Zygotic and Early Embryo Reprogrammingmentioning

confidence: 99%

Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri‐implantation embryos

et al. 2018

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MicroRNAs (miRNAs) are active regulators of numerous biological and physiological processes including most of the events of mammalian reproduction. Understanding the biological functions of miRNAs in the context of mammalian reproduction will allow a better and comparative understanding of fertility and sterility in male and female mammals. Herein, we summarize recent progress in miRNA-mediated regulation of mammalian reproduction and highlight the significance of miRNAs in different aspects of mammalian reproduction including the biogenesis of germ cells, the functionality of reproductive organs, and the development of early embryos. Furthermore, we focus on the gene expression regulatory feedback loops involving hormones and miRNA expression to increase our understanding of germ cell commitment and the functioning of reproductive organs. Finally, we discuss the influence of miRNAs on male and female reproductive failure, and provide perspectives for future studies on this topic.

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Highly sensitive sequencing reveals dynamic modifications and activities of small RNAs in mouse oocytes and early embryos

Abstract: Expression, modification, and activity of microRNAs are dynamically regulated in early mouse embryos unveiled by sensitive sequencing.

Cited by 110 publications

References 75 publications

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

microRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles

Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri‐implantation embryos

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