Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri‐implantation embryos

Reza, Abu Musa Md Talimur; Choi, Yun‐Jung; Han, Sung Gu; Song, Hyuk; Park, Chankyu; Hong, Kwonho; Kim, Jin‐Hoi

doi:10.1111/brv.12459

Cited by 107 publications

(103 citation statements)

References 273 publications

(419 reference statements)

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“…The relevance of miRNAs to ovarian aging and POI has been investigated previously (Esteller, 2011; Lee & Young, 2013; Reza et al, 2019). Transcriptional landscape of aged‐mouse ovaries discovered a unique set of miRNAs associated with physiological and environmental ovarian dysfunctions, suggesting miRNAs as mediators of the effects of age, genes, and environment on ovarian aging (Cuomo & Ambrosino, 2019; Cuomo et al, 2018).…”

Section: Discussionmentioning

confidence: 98%

MicroRNA‐127‐5p impairs function of granulosa cells via HMGB2 gene in premature ovarian insufficiency

Zhang

Dang

Liu

et al. 2020

Journal Cellular Physiology

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Distinct microRNA (miRNA) profiles have been reported in premature ovarian insufficiency (POI), but their functional relevance in POI is not yet clearly stated. In this study, aberrant expressions of miR‐127‐5p and high mobility group box 2 (HMGB2) were observed by microarrays in granulosa cells (GCs) from biochemical POI (bPOI) women and further confirmed by a quantitative reverse‐transcription polymerase chain reaction. Immortalized human granulosa cell line and mouse primary ovarian GCs were used for functional validation. Orthotopic mouse model was established to examine the role of miR‐127‐5p in vivo. Finally, the expression of miR‐127‐5p was measured in the plasma of bPOI women. The receiver operating characteristic curve analysis was performed to determine the indicative role of miR‐127‐5p for ovarian reserve. Results showed the upregulation of miR‐127‐5p was identified in GCs from bPOI patients. It inhibited GCs proliferation and impaired DNA damage repair capacity through targeting HMGB2, which was significantly downregulated in GCs from the same cohort of cases. miR‐127‐5p was confirmed to attenuate DNA repair capability via HMGB2 in mouse ovary in vivo. Intriguingly, the upexpression of miR‐127‐5p was also detected in plasma of bPOI individuals, suggesting that miR‐127‐5p could be a promising indicator for bPOI. Taken together, our results discovered the deleterious effects of miR‐127‐5p on GCs function and its predictive value in POI process. The target gene HMGB2 could be considered as a new candidate for POI. This study highlights the importance of DNA repair capacity for ovarian function and sheds light on the epigenetic mechanism in the pathogenicity of POI.

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Section: Discussionmentioning

confidence: 98%

MicroRNA‐127‐5p impairs function of granulosa cells via HMGB2 gene in premature ovarian insufficiency

Zhang

Dang

Liu

et al. 2020

Journal Cellular Physiology

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“…Among the small ncRNAs, tRNAs, small nucleolar and nuclear RNAs have been identified as the small housekeeping ncRNAs, instead PIWI-interacting RNAs (piRNAs) and microRNAs (miRNAs) are known as small regulatory ncRNAs. In addition to miRNAs and piRNAs [44,45], tRNA-derived small RNAs (tsRNAs) populate mature SPZ [46,47]. These are 5 fragments of tRNAs with a size from 29 to 34 nt, whose biogenesis still remains largely unknown.…”

Section: Sperm Rna Codementioning

confidence: 99%

Histone Post-Translational Modifications and CircRNAs in Mouse and Human Spermatozoa: Potential Epigenetic Marks to Assess Human Sperm Quality

Chioccarelli

Pierantoni

Manfrevola

et al. 2020

JCM

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Spermatozoa (SPZ) are motile cells, characterized by a cargo of epigenetic information including histone post-translational modifications (histone PTMs) and non-coding RNAs. Specific histone PTMs are present in developing germ cells, with a key role in spermatogenic events such as self-renewal and commitment of spermatogonia (SPG), meiotic recombination, nuclear condensation in spermatids (SPT). Nuclear condensation is related to chromatin remodeling events and requires a massive histone-to-protamine exchange. After this event a small percentage of chromatin is condensed by histones and SPZ contain nucleoprotamines and a small fraction of nucleohistone chromatin carrying a landascape of histone PTMs. Circular RNAs (circRNAs), a new class of non-coding RNAs, characterized by a nonlinear back-spliced junction, able to play as microRNA (miRNA) sponges, protein scaffolds and translation templates, have been recently characterized in both human and mouse SPZ. Since their abundance in eukaryote tissues, it is challenging to deepen their biological function, especially in the field of reproduction. Here we review the critical role of histone PTMs in male germ cells and the profile of circRNAs in mouse and human SPZ. Furthermore, we discuss their suggested role as novel epigenetic biomarkers to assess sperm quality and improve artificial insemination procedure.

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“…It can be assumed that the sperm histone code is sensitive to endocrine disruptors, with effect similar to estrogens, as well as to the involvement of estrogen receptors in histone code establishment [15]. Moreover, the negative role of environmental pollutants in the influence on noncoding RNAs in spermatozoa, another tool of epigenetic regulation [87] with ability to drive epigenetic inheritance [88], is wellknown [89,90].…”

Section: Molecular Mechanism Of Eds In Extremely Low Dosesmentioning

confidence: 99%

Endocrine Disruptors: Very Low Doses with Genuinely High Impacts on Male Reproduction

Ješeta¹,

Nevoral²

2020

Male Reproductive Health

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Endocrine disruptors (EDs) are chemical substances that affect physiological processes in the body via hormonal regulation. They are often detected in food, plastic water bottles, cosmetics, and many other daily need items. Thereafter, EDs are detected in many bodily fluids, pointing out the real exposure to even very low doses. Permanent and long-term utilization of EDs has harmful effects on male reproductive health mainly due to interference with sex hormone synthesis and mechanism of action. However, with decreasing dosage of EDs, the possibilities of unpredictable modes of action arise. In addition to various molecular actions of individual EDs, the interference of individual ones represents another dimension of the ED issue. This review provides an overview of the EDs and their possible impact on reproductive health in males, with focus on sperm quality with the mighty potential of epigenetic transmission to further generations. The "posttranslational" effect of EDs in really low doses in real exposure routes is stigmatized in this review, being strongly considered as creeping molecular action of individual EDs as well as amplifications of their copresence in the environment.

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Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri‐implantation embryos

Cited by 107 publications

References 273 publications

MicroRNA‐127‐5p impairs function of granulosa cells via HMGB2 gene in premature ovarian insufficiency

MicroRNA‐127‐5p impairs function of granulosa cells via HMGB2 gene in premature ovarian insufficiency

Histone Post-Translational Modifications and CircRNAs in Mouse and Human Spermatozoa: Potential Epigenetic Marks to Assess Human Sperm Quality

Endocrine Disruptors: Very Low Doses with Genuinely High Impacts on Male Reproduction

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