Highly Sensitive Molecularly Imprinted Sensor Based on Platinum Thin‐film Microelectrode for Detection of Chloramphenicol in Food Samples

Abstract

A highly sensitive and selective electrochemical biomimetic sensor was fabricated for fast detection of chloramphenicol (CAP) in honey and milk samples. Platinum thin‐film microelectrode (Pt TFME), which could provide unique electrochemical properties and achieve measurement using very limited solution volumes, was surface‐modified by electropolymerizing o‐phenylenediamine. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the preparation process of CAP‐imprinted poly(o‐phenylenediamine) film and rebinding ability of CAP into the imprinted cavities. The electrochemical properties of the sensor were further investigated with square wave voltammetry (SWV) by using K3Fe(CN)6 as an electroactive probe. The current difference of oxidation peaks of K3Fe(CN)6 had a good linear relationship with the concentration of CAP in the range of 0.9–10 nM. The detection limit was 0.39 nM based on the signal to noise ratio of 3. The developed sensor was successfully applied to determine CAP in honey and milk samples, and the result was in good agreement with that obtained by high performance liquid chromatography‐mass spectrometry (HPLC‐MS). The sensor showed high sensitivity and excellent selectivity to CAP in comparison to other structurally related and/or normally existing antibiotics, and demonstrated great promise for the rapid quantification of CAP in real food samples and field analysis.

Green preparation of chlorine-doped graphene and its application in electrochemical sensor for chloramphenicol detection

“…Alternatively, electrochemical method has the advantages of rapid analysis, cheap equipment and simple operation [8,9]. Thus many electrochemical sensors for CAP detection based on various electrode materials have been developed [10][11][12][13][14][15][16][17]. Among these electrode materials, various nanomaterials such as carbon and metal nanoparticles, have been employed for antibiotics drug residues detection in animal-derived food and water samples [18][19][20][21].…”

Honey Evaluation Using Electronic Tongues: An Overview

“…From a qualitative (i.e., classification/discrimination) point of view, the majority of the literature works addressed the possibility of classifying honey samples according to the botanical or geographical origins as well as to identify honey adulterations or the adulteration level [81,91,[103][104][105][106][107][108][109][110][111][112][113]. A substantial number of works reported the satisfactory quantitative performance of voltammetric E-tongues used to predict chemical and biochemical honey composition as well as the levels of adulterants and/or contaminants [91,104,106,[113][114][115][116][117][118][119][120][121][122][123][124][125]. As can be easily inferred from Table 3 (commercial devices) and Tables 4 and 5 (self-assembled lab-made conventional or multi-sensors devices), the use of voltammetric E-tongues for honey analysis is a more recent practice (from 2011) compared to the potentiometric approaches (from 2008) being largely used together with different multivariate statistical techniques (e.g., multiple linear regression models (MLRM), PLS, ANN, among others) as successful quantitative analytical tools.…”

“…Only one work reported the use of a commercial conventional three-component device [114]. In contrast all the other studies, reported, as previously stated, the development and/or use of lab-made devices comprising a single WE [81,[105][106][107][108][109][110][111][115][116][117][118][119][120][121][122][123][124][125] or more WEs [91,103,104,112,113], some of them modified incorporated porous films or nanoparticles [99][100][101]115,[117][118][119][120][121][122][123][124][125]. Within these applications, different voltammetric techniques have been applied namely cyclic voltammetry (CV, the most common), square-wave voltammetry (SWV) and square-wave cathodic stripping voltammetry (SWCSV), differential pulse voltammetry (DPV) and multifrequency large amplitude pulse voltammetry (MLAPV) as well as linear sweep voltammetry (LSV).…”

Electrosynthesis of Molecularly Imprinted Poly‐o‐phenylenediamine on MWCNT Modified Electrode for Selective Determination of Meldonium

“…MIPs are artificial polymers formed upon polymerization of complex structure that was produced by interaction between target molecule and functional monomers. The extraction of the template from the polymer backbone creates molecular cavities which have supplemental shape and size of the template, so the target molecules could be recognized selectively through the cavities in any matrix of interest . The most used methods for the preparation of MIPs are photopolymerization, electropolymerization and free radical polymerization .…”