Highly sensitive microbial biosensor based on recombinant Escherichia coli overexpressing catechol 2,3-dioxygenase for reliable detection of catechol

Liu, Zhuang; Zhang, Yachao; Bian, Congcong; Xia, Tian; Gao, Yan; Zhang, Xueli; Wang, Huimin; Ma, Hanyue; Hu, Yifeng; Wang, Xia

doi:10.1016/j.bios.2018.10.050

Cited by 35 publications

(15 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous work, it was found that NPG with bicontinuous three-dimensional nanoporous structure (as shown in Figure 1a and Figure 2a) was an ideal supporting material for the immobilization of bio-recognition elements such as enzymes or microbial cells 29,30,31 . Moreover, as a nanoporous metal material, NPG has an excellent electrical conductivity and efficient electrocatalytic activity towards many substrates, such as catechol 31 and NADH 32 . These excellent properties of NPG can be utilized in the construction of the multi-biocatalyst sensing chip for pathogenic (which was not certified by peer review) is the author/funder.…”

Section: Construction Principle Of the Triple Functional Sensing Chipmentioning

confidence: 99%

“…The pore-forming phenomenon will occur when LLO protein adsorbs on the membrane of the Cat-Lipo, which can cause catechol release from the Cat-Lipo (Figure 1b). Then, the released catechol can be catalyzed by NPG generating a current signal as shown in Figure 1b, and the working principle could be expressed as follows 31…”

Section: Construction Principle Of the Triple Functional Sensing Chipmentioning

confidence: 99%

“…XhoI site underlined). The procedure of heterologous expression referred to our previous report31 . For AR expression, the bdha gene was amplified by PCR using Bacillus subtilis genomic DNA as a template.…”

mentioning

confidence: 99%

See 2 more Smart Citations

A triple functional sensing chip for rapid detection of pathogenicListeria monocytogenes

Zhang

Wang

Xiao

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Here a triple functional sensing chip was created for L. monocytogenes detection by integrating three biomarkers (Listeriolysin O (LLO) at protein level, hly gene at genetic level, and acetoin at metabolic level). Liposome encapsulated catechol was used for LLO detection via LLO pore-forming ability. hly gene was specifically captured by using a thiolated capture probe on nanoporous gold (NPG). As an electroactive label, methylene blue was embedded in double-stranded structures to generate an electrochemical signal for hly detection. Combined with the electrocatalysis of NADH by NPG, the acetoin detection was achieved by measuring the consumption of NADH as a cofactor under acetoin reductase catalysis. Importantly, the L. monocytogenes detection results obtained by detecting three biomarkers using the chip can be mutually verified, which reduces the probability of false positives based on a single marker. Moreover, the detection time was reduced to about 90 min, making it a rapid and reliable tool for L. monocytogenes detection.

show abstract

Section: Construction Principle Of the Triple Functional Sensing Chipmentioning

confidence: 99%

Section: Construction Principle Of the Triple Functional Sensing Chipmentioning

confidence: 99%

See 1 more Smart Citation

A triple functional sensing chip for rapid detection of pathogenicListeria monocytogenes

Zhang

Wang

Xiao

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…This gene was cloned and over-expressed in E. coli BL21 that proved to have higher catalytic activity towards catechol electrochemical detection, the whole cells presence providing a better stability for C23O when compared with simple enzyme. NPG has unique structural properties and was successfully selected as a support for the immobilization of the recombinant E. coli BL21 over-expressed C23O and revealed a good stability (Liu et al 2019).…”

Section: Metallic-based Nanomaterials As Platform For Cell Biosensorsmentioning

confidence: 99%

New Materials for the Construction of Electrochemical Cell-Based Biosensors

Cernat¹,

Ciui²,

Fritea³

et al. 2020

Handbook of Cell Biosensors

View full text Add to dashboard Cite

show abstract

“…In the present paper, a simple yet efficient bacterial model based on Escherichia coli is utilized. Recently, it was used to develop a biosensor for detection of catechol [19] and to detect biofilm formation using localized surface plasmon resonance in real-time [20]. Here, we decided to apply an E. coli biosensor based on the MG1655 pKatG-lux strain, which is specific to one of the main reactive oxygen species—hydrogen peroxide (H 2 O 2 ).…”

Section: Introductionmentioning

confidence: 99%

Biological Effects of C60 Fullerene Revealed with Bacterial Biosensor—Toxic or Rather Antioxidant?

et al. 2019

View full text Add to dashboard Cite

Nanoparticles have been attracting growing interest for both their antioxidant and toxic effects. Their exact action on cells strongly depends on many factors, including experimental conditions, preparation, and solvents used, which have contributed to the confusion regarding their safety and possible health benefits. In order to clarify the biological effects of the most abundant fullerene C60, its impact on the Escherichia coli model has been studied. The main question was if C60 would have any antioxidant influence on the cell and, if yes, whether and to which extent it would be concentration-dependent. An oxidative stress induced by adding hydrogen peroxide was measured with an E. coli MG1655 pKatG-lux strain sensor, with its time evolution being recorded in the presence of fullerene C60 suspensions of different concentrations. Optimal conditions for the fullerene C60 solubilization in TWEEN 80 2% aqueous solution, together with resulting aggregate sizes, were determined. Results obtained for the bacterial model can be extrapolated on eukaryote mitochondria. The ability of C60 to penetrate through biological membranes, conduct protons, and interact with free radicals is likely responsible for its protective effect detected for E. coli. Thus, fullerene can be considered as a mitochondria-targeted antioxidant, worth further researching as a prospective component of novel medications.

show abstract

Highly sensitive microbial biosensor based on recombinant Escherichia coli overexpressing catechol 2,3-dioxygenase for reliable detection of catechol

Cited by 35 publications

References 31 publications

A triple functional sensing chip for rapid detection of pathogenicListeria monocytogenes

A triple functional sensing chip for rapid detection of pathogenicListeria monocytogenes

New Materials for the Construction of Electrochemical Cell-Based Biosensors

Biological Effects of C60 Fullerene Revealed with Bacterial Biosensor—Toxic or Rather Antioxidant?

Contact Info

Product

Resources

About