Highly sensitive method for the determination of 5-fluorouracil in biological samples in the presence of 2'-deoxy-5-fluorouridine by gas chromatography—mass spectrometry

Kubo, Michinori; Sasabe, Hiroyuki; Shimizu, Takefumi

doi:10.1016/0378-4347(91)80076-o

Cited by 17 publications

(3 citation statements)

References 23 publications

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“…5-FU was assessed by electron-impact ionization GC-MS technique within the linear range of 0-100 ng mL -1 , without any interference of 2 0 -deoxy-5-flurouridine. This method was validated in rat plasma samples (LOD: 0.5 ng mL -1 ) [10]. Plasma 5-FU concentrations have been determined by GC-MS technique in gastric and colorectal cancer patients receiving Ura and tegafur or oral doxifluridine.…”

Section: Hybrid Instrumental Techniques For 5-fu Analysis In Biologicmentioning

confidence: 99%

“…Many opportunities for applications particularly molecularly imprinted polymer (MIP) as chromatographic, LLE, SPE, and SPME materials for Ura and 5-FU analysis are still to be exploited. Depending on the origins of samples and analytical objectives, Ura and 5-FU analysis have been carried out using various analytical instruments in many situations such as clinical control for diagnosis and treatment of diseases, pharmacology, and toxicology [1][2][3][4][8][9][10]. In the next section, we would like to contemplate on the current chromatographic methods developed for the purposes of separating the Ura and 5-FU from complex samples containing interferents and detecting them once separation is complete (Fig.…”

Section: Introductionmentioning

confidence: 99%

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A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil

2015

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The most important objectives that are frequently found in bio-analytical chemistry involve applying tools to relevant medical/biological problems and refining these applications. Developing a reliable sample preparation step, for the medical and biological fields is another primary objective in analytical chemistry, in order to extract and isolate the analytes of interest from complex biological matrices. Since, main inborn errors of metabolism (IEM) diagnosable through uracil analysis and the therapeutic monitoring of toxic 5-fluoruracil (an important anticancerous drug) in dihydropyrimidine dehydrogenase deficient patients, require an ultra-sensitive, reproducible, selective, and accurate analytical techniques for their measurements. Therefore, keeping in view, the diagnostic value of uracil and 5-fluoruracil measurements, this article refines several analytical techniques involved in selective recognition and quantification of uracil and 5-fluoruracil from biological and pharmaceutical samples. The prospective study revealed that implementation of molecularly imprinted polymer as a solid-phase material for sample preparation and preconcentration of uracil and 5-fluoruracil had proven to be effective as it could obviates problems related to tedious separation techniques, owing to protein binding and drastic interferences, from the complex matrices in real samples such as blood plasma, serum samples.

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Section: Hybrid Instrumental Techniques For 5-fu Analysis In Biologicmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil

2015

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“…The latter is known to be broken down into 5-FU during derivatization for GC. However, Kubo et al (1991) reported mild reaction conditions (30 min, r.t.) to convert these compounds into their tert-butyldimethylsilyl derivatives with no FdUrd being converted into 5-FU. The bicyclic antidepressant, fluoxitine (FX) was determined in either postmortem biological samples (Crifasi et al, 1997) or rat tissues such as brain, blood, liver and hair (Lefebvre et al, 1999).…”

Section: Ms As a Detector For Gc Hplc And Ce Gc-msmentioning

confidence: 99%

Hyphenated chromatographic methods for biomaterials

Al-Dirbashi¹,

Nakashima²

2000

Biomed. Chromatogr.

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The improvement in hyphenated analytical techniques has significantly widened their applications to the analysis of biomaterials. In this article, we discuss recent advances in applications of hyphenated chromatographic techniques including capillary electrophoresis to the analyses of biological samples. As tools of separation, gas chromatography, high-performance liquid chromatography and capillary electrophoresis are considered with special emphasis on applications utilizing the hyphenation of these methods to mass spectrometry. Moreover, applications using other detection methods such as Fourier transform infrared spectroscopy hyphenated to gas chromatography and photodiode array detector combined with high-performance liquid chromatography or capillary electrophoresis are also discussed. Owing to their high sensitivity, luminescence-based detection systems such as laser-induced fluorescence and chemiluminescence are also included in this review.

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A review of analytical methods for the determination of 5-fluorouracil in biological matrices

Breda¹,

Barattè²

2010

Anal Bioanal Chem

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5-Fluorouracil (5-FU) is a cytostatic agent that has been widely used in the treatment of various solid tumours for more than 20 years, and is still considered to be among the most active antineoplastic agents in advanced colorectal cancer and malignancies of the head and neck. A large number of non-chromatographic and chromatographic methods for the quantitation of 5-FU, related prodrugs and their metabolites in biological matrices have been developed in the last 30 years to support preclinical and clinical studies. However, 5-FU monitoring has not been widely used, at least not in the USA, and certainly not outside the clinical research setting, given the absence of simple, fast and inexpensive testing methods for 5-FU monitoring. Recent developments with testing based on liquid chromatography-tandem mass spectrometry and a nanoparticle antibody-based immunoassay may facilitate routine monitoring of 5-FU in daily clinical practice. In this review the advantages and disadvantages of the bioanalytical methods developed and used for 5-FU, its metabolites and related prodrugs are discussed.

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Highly sensitive method for the determination of 5-fluorouracil in biological samples in the presence of 2'-deoxy-5-fluorouridine by gas chromatography—mass spectrometry

Cited by 17 publications

References 23 publications

A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil

A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil

Hyphenated chromatographic methods for biomaterials

A review of analytical methods for the determination of 5-fluorouracil in biological matrices

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