A review of analytical methods for the determination of 5-fluorouracil in biological matrices

Breda, Massimo; Barattè, S.

doi:10.1007/s00216-010-3633-8

Cited by 38 publications

(37 citation statements)

References 93 publications

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“…Several analytical techniques have been developed for quantitating 5-FU and related compounds [43]. In the 1960’s, cell-based culture assays were developed to measure 5-FU levels, and these assays were subsequently replaced with gas chromatography in the mid-1970’s.…”

Section: Quantitation Of Plasma 5-fu Levelsmentioning

confidence: 99%

Therapeutic drug monitoring of 5-fluorouracil

Lee

Beumer

Chu

2016

Cancer Chemother Pharmacol

172

147

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Purpose For over 50 years, 5-FU has played a critical role in the systemic chemotherapy of cancer patients. 5-FU serves as the main backbone of combination chemotherapy for patients with colorectal cancer (CRC) in both the adjuvant and metastatic disease settings. Herein, we review the current status of 5-FU therapeutic drug monitoring (TDM) and discuss its potential role in the clinical practice setting. Method PubMed and abstracts from the American Society of Clinical Oncology (ASCO) were searched up through September 2015 for clinical data relating to 5-FU TDM. Results 5-FU dosing has been typically determined by using body surface area (BSA). However, it is now well-established that BSA-based 5-FU dosing is correlated with a wide variation of 5-FU systemic exposure. Pharmacokinetic (PK) studies of 5-FU systemic exposure have shown a wide range of interpatient variation of 5-FU plasma drug levels. Over the past 30 years, increasing efforts have been placed on optimizing 5-FU dosing with the main goals of increasing antitumor efficacy while reducing drug-associated toxicity. There is growing evidence to show that 5-FU dosing based on plasma 5-FU drug level is feasible and that 5-FU TDM can improve clinical outcomes by improving efficacy of 5-FU-based combination regimens and reducing toxicities. Conclusion Dose adjustment of 5-FU is feasible and PK-based dosing can significantly improve clinical outcomes by reducing toxicities and improving efficacy.

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Section: Quantitation Of Plasma 5-fu Levelsmentioning

confidence: 99%

Therapeutic drug monitoring of 5-fluorouracil

Lee

Beumer

Chu

2016

Cancer Chemother Pharmacol

172

147

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“…5 ‐flurouarcil (5 ‐ FU) a derivative of uracil in which the 5 th positioned hydrogen is replaced by fluorine, is used as an excellent antineoplastic agent in treatment of cancer such as colorectal, breast, stomach, pancreatic, and cervical . One of the major mechanisms responsible for the anti‐tumor activity of 5 ‐ FU is inhibition of thymidylate synthetase . To affect a pharmacological activity against tumor it is necessary for drug concentration to be high.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensor for the Determination of Anticancer Drug 5- Fluorouracil at Glucose Modified Electrode

Bukkitgar¹,

Shetti

2016

ChemistrySelect

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In the present work, the complex oxidation mechanism of glucose at the surface of carbon paste electrode was utilized for the electro deposition of glucose. As compared to the bare electrode there was increase in the peak current, which would enhance the sensitivity and selectivity of the electrode. The electro-catalytic activity of the novel electrode was investigated for the oxidation of anticancer drug 5 -fluorouracil. The observed results indicated the applicability of electrode for electrochemical investigation of 5 -fluorouracil with limit of detection and limit of quantification 5.17 nm and 17.2 nM.Investigation for influence of scan rate was used to underatand the electrode process of 5 -fluorouracil. It was observed that it undegoes diffusion controlled process with transfer of two protons and two electrons respectively. In addition, a differential pulse voltammetric technique was developed for the determination of 5 -fluorouracil. The developed method was used for determination of 5 -fluorouracil in pharmaceutical dosage and urine samples. Good recovery from biological sample such as urine indicates the applicability of the proposed method for clinical trials.[a] S.

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“…It is still considered to be among the most effective anti-neoplastic agents in advanced colorectal cancer and malignancies of the head and neck. 1) Many non-chromatographic and chromatographic methods for the quantitation of 5-FU, related pro-drugs, and metabolites in biological matrices have been developed over the last 30 years to support preclinical and clinical studies.…”

mentioning

confidence: 99%

Rebamipide Attenuates 5-Fluorouracil-Induced Small Intestinal Mucositis in a Mouse Model

Kim

Moon

et al. 2015

Biological & Pharmaceutical Bulletin

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5-Fluorouracil (5-FU)-induced intestinal mucositis is one of the most common morbidities in chemother-apy and involves the reactive oxygen species (ROS) system, apoptosis, and inflammatory cytokines. Rebamipide exerts a mucosal-protective effect, mediated through several mechanisms. The aim of this study was to evaluate the effects of rebamipide in 5-FU-induced mouse small-intestinal mucositis. BALB/c mice were assigned randomly to four groups; (1) control group (n 10; receiving saline orally for 6 d), (2) Key words mucositis; 5-fluorouracil; small intestine; rebamipide 5-Fluorouracil (5-FU) is a cytostatic agent that has been used widely in the treatment of various solid tumors for more than 20 years. It is still considered to be among the most effective anti-neoplastic agents in advanced colorectal cancer and malignancies of the head and neck.1) Many non-chromatographic and chromatographic methods for the quantitation of 5-FU, related pro-drugs, and metabolites in biological matrices have been developed over the last 30 years to support preclinical and clinical studies.2) Nevertheless, 50-80% of patients who undergo 5-FU chemotherapy show clinical manifestations of mucositis, symptoms of which include severe diarrhea.2) Its incidence in patients who underwent standarddose chemotherapy was 40%, while it approached 100% in patients treated with high-dose chemotherapy.3)The pathophysiological mechanism of 5-FU-induced mucositis comprises five phases 4)

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A review of analytical methods for the determination of 5-fluorouracil in biological matrices

Cited by 38 publications

References 93 publications

Therapeutic drug monitoring of 5-fluorouracil

Therapeutic drug monitoring of 5-fluorouracil

Electrochemical Sensor for the Determination of Anticancer Drug 5- Fluorouracil at Glucose Modified Electrode

Rebamipide Attenuates 5-Fluorouracil-Induced Small Intestinal Mucositis in a Mouse Model

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