Highly sensitive label-free electrochemical aptasensors based on photoresist derived carbon for cancer biomarker detection

Forouzanfar, Shahrzad; Alam, Fahmida; Pala, Nezih; Wang, Chunlei

doi:10.1016/j.bios.2020.112598

Cited by 36 publications

(27 citation statements)

References 43 publications

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“…The PDGF-BB binding aptamer (ssDNA, amino linker-5 -C 6 -CAG GCT ACG GCA CGT AGA GCA TCA CCA TGA TCC TG-3 [14]) was purchased in HPLC purification from ThermoFisher Scientific, Waltham, MA, USA. Tris-ethylenediaminetetraacetic acid (TE) buffer, ethanol, acetone, phosphate-buffered saline (1 M and pH 7.4) (PBS), 1-ethyl-3-(3dimethylaminopropyl) carbodiimide hydrochloride linker (EDC), N-hydroxysuccinimide linker (NHS), hydrochloric acid (HCl), Polyoxyethylene (20) sorbitan monolaurate (Tween-20), KCl, and k 3 Fe(CN) 6 were purchased from ThermoFisher Scientific, Waltham, MA, USA.…”

Section: Materials and Reagentsmentioning

confidence: 99%

“…The 3D C-MEMS microelectrodes were fabricated via the previously reported C-MEMS synthesis process [3,14,36,37], schematically illustrated in Figure 1a. Briefly, the fabrication process includes producing a uniform layer of SU-8 25 with the thickness of 15 µm on a 4 N-doped silicon wafer via spinning at 3000 rpm for 30 s, performing pre-exposure bakes at the recommended temperature, UV exposure with a dose of 300 mJ cm −2 , and post-exposure bakes.…”

Section: D C-mems Photolithographymentioning

confidence: 99%

“…Previous studies have thoroughly discussed the deployed aptasensor development [14,15]. The process includes activating the amino-tags of aptamers in a solution of 20 mg mL −1 EDC and 10 µL of 20 mg mL −1 NHS and immobilizing the aptamers via the drop-casting method.…”

Section: Aptasensor Developmentmentioning

confidence: 99%

“…Aptamer-based biosensors (also known as aptasensors) are a well-known type of electrochemical sensor in which aptamers are used for the detection of the bio targets (e.g., cancer biomarkers) [12,13]. Aptamers can provide several advantages over the other detection methods (e.g., enzymatic and immunosensing), including the high yield of fabrication, ease of manipulation (e.g., immobilization), low cost of synthesis, and high affinity and selectivity [4,14]. The suitability of electrochemical aptasensors for POC biosensing can be further explained by the fact that the cost of fabrication and operation can be considerably reduced.…”

Section: Introductionmentioning

confidence: 99%

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In-Situ Integration of 3D C-MEMS Microelectrodes with Bipolar Exfoliated Graphene for Label-Free Electrochemical Cancer Biomarkers Aptasensor

2022

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The electrochemical label-free aptamer-based biosensors (also known as aptasensors) are highly suitable for point-of-care applications. The well-established C-MEMS (carbon microelectromechanical systems) platforms have distinguishing features which are highly suitable for biosensing applications such as low background noise, high capacitance, high stability when exposed to different physical/chemical treatments, biocompatibility, and good electrical conductivity. This study investigates the integration of bipolar exfoliated (BPE) reduced graphene oxide (rGO) with 3D C-MEMS microelectrodes for developing PDGF-BB (platelet-derived growth factor-BB) label-free aptasensors. A simple setup has been used for exfoliation, reduction, and deposition of rGO on the 3D C-MEMS microelectrodes based on the principle of bipolar electrochemistry of graphite in deionized water. The electrochemical bipolar exfoliation of rGO resolves the drawbacks of commonly applied methods for synthesis and deposition of rGO, such as requiring complicated and costly processes, excessive use of harsh chemicals, and complex subsequent deposition procedures. The PDGF-BB affinity aptamers were covalently immobilized by binding amino-tag terminated aptamers and rGO surfaces. The turn-off sensing strategy was implemented by measuring the areal capacitance from CV plots. The aptasensor showed a wide linear range of 1 pM–10 nM, high sensitivity of 3.09 mF cm−2 Logc−1 (unit of c, pM), and a low detection limit of 0.75 pM. This study demonstrated the successful and novel in-situ deposition of BPE-rGO on 3D C-MEMS microelectrodes. Considering the BPE technique’s simplicity and efficiency, along with the high potential of C-MEMS technology, this novel procedure is highly promising for developing high-performance graphene-based viable lab-on-chip and point-of-care cancer diagnosis technologies.

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Section: Materials and Reagentsmentioning

confidence: 99%

Section: D C-mems Photolithographymentioning

confidence: 99%

Section: Aptasensor Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In-Situ Integration of 3D C-MEMS Microelectrodes with Bipolar Exfoliated Graphene for Label-Free Electrochemical Cancer Biomarkers Aptasensor

2022

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“…Since their introduction in the 1990s, 69 aptamers have been utilized for detecting various biomarkers, including cardiac, cancer, viral, and bacterial biomarkers. [70][71][72][73] So far, aptamers are utilized as single receptor and sandwich assays with and without labeling for detecting cardiac biomarkers (Figure 2(d)-(g)). 70 In label-free single-receptor aptasensors, various functionalized aptamers (e.g., -SH and -NH 2 ) with specific DNA/RNA sequences are immobilized onto the transducer surface via SAM and covalent bonding.…”

Section: Principles Of the Electrochemical Detection Of Cardiac Biomarkersmentioning

confidence: 99%

Advances in electrochemical aptasensing for cardiac biomarkers

Rahman

Lopa

Lee

2021

Bulletin Korean Chem Soc

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Novel bio-receptors or bio-recognition molecular markers with high specificity, stability, and affinity for the efficient binding and entrapment of target biomolecules are essential for developing biomedical devices with high sensitivity and reliability. Aptamers are peptide or nucleic-acid (DNA or RNA) molecules capable of binding to target biomarkers by forming a unique three-dimensional structure, providing an effective platform for biosensing. This review comprehensively summarizes the progress of label-free and label-based electrochemical aptasensors for the detection of various biomarkers related to cardiovascular diseases. It also outlines the fundamental properties of different cardiac biomarkers, synthesis of target-specific aptamers, and working principles, such as single receptor and sandwich, of the different types of sensing assays for the electrochemical detection of cardiac biomarkers while highlighting the current challenges and future perspectives.

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Nanofabrication and Sensing Technology: from the Interface‐Mediated Mechanism Point‐of‐View

Kar,

Guerra,

Liao

2024

Advanced Sensor Research

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In recent years, the field of sensing technology has experienced notable advancements, where sensing devices have emerged as pivotal tools in enhancing operational efficiency, cutting costs, and bolstering security across diverse sectors. In this context, the preparation of nanoscale materials and structures, including colloidal particle synthesis and lithographic structure fabrication will be discussed. The significance of managing the interface environment in sensor designs, with nanofabrication advancements enabling the development of innovative sensing devices, is highlighted. Control over the interface environment is argued not only dictates the capability of sensor designs but also opens avenues for next‐generation sensor fabrication and integration. By focusing on the interface‐mediated mechanism, this approach offers a comprehensive roadmap of this research area, its challenges and potential solutions, and prospective opportunities.

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Highly sensitive label-free electrochemical aptasensors based on photoresist derived carbon for cancer biomarker detection

Cited by 36 publications

References 43 publications

In-Situ Integration of 3D C-MEMS Microelectrodes with Bipolar Exfoliated Graphene for Label-Free Electrochemical Cancer Biomarkers Aptasensor

In-Situ Integration of 3D C-MEMS Microelectrodes with Bipolar Exfoliated Graphene for Label-Free Electrochemical Cancer Biomarkers Aptasensor

Advances in electrochemical aptasensing for cardiac biomarkers

Nanofabrication and Sensing Technology: from the Interface‐Mediated Mechanism Point‐of‐View

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