Highly sensitive interference-free electrochemical determination of pyridoxine at graphene modified electrode: Importance in Parkinson and Asthma treatments

In this study; a sensitive, selective, and simple electrochemical sensor was developed to determine low concentration pyridoxine (Py) using a phosphorus‐doped pencil graphite electrode (P‐doped/PGE). Electrode modification was implemented using the chronoamperometry method at +2.0 V constant potential and 100 seconds in 0.1 mol L−1 H3PO4 supporting electrolyte solution. The characterization processes of the P‐doped/PGE were carried out using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), X‐ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM), energy‐dispersive X‐ray spectroscopy (EDX), and atomic force microscope (AFM) methods. In the concentration study, using the differential pulse voltammetry (DPV) method, a linear calibration plot was acquired in the concentration range of 0.5 to 300 μmol L−1 Py. The limit of quantification (LOQ) and limit of detection (LOD) of the developed method were calculated as 0.219 μmol L−1 and 0.0656 μmol L−1, respectively. Detection of Py has been successfully performed on the P‐doped/PGE in the beverage samples. As a result, the method developed has been shown to have fast, low cost, and simple for the sensitive and selective detection of Py as an effective electrode.

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“…After that, the na was calculated as 1.62, which is approximately 2.0. The reaction taking place is as in Figure 4 [3,5,40,43,48].…”

Section: Kinetic Studies Of Py At the P-doped/pgementioning

confidence: 99%

“…4. The electrochemical oxidation reaction of Py [3,5,40,43,48]. doped/PGE has some important advantages over other electrochemical methods described in the literature, such as simplicity (one-step preparation), speed, and low cost.…”

Section: Analytical Applicationmentioning

confidence: 99%

Cost‐effective and Facile Production of a Phosphorus‐doped Graphite Electrode for the Electrochemical Determination of Pyridoxine

2021

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“…Therefore, regular monitoring of DA levels in human body is necessary . Pyridoxine (PY), otherwise known as vitamin B 6 is a water soluble vitamin which plays significant role in body cells maintenance and in amino acids metabolism . Three forms of PY are available which are known as, pyridoxal, pyridoxol and pyridoxamine .…”

Section: Introductionmentioning

confidence: 99%

“…It is reported that vitamin B 6 imposes major effects on gene modulation, sexual behaviour, and immune modulation in HIV‐1 infection . Insufficient concentration level of PY in body leads to illness and various sicknesses such as hypertension, cardiovascular problems, hyperlipidemia, obesity and anaemia . Hence, accurate PY determination is essential in human health diagnostics.…”

Section: Introductionmentioning

confidence: 99%

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Sensitive Determination of Acetaminophen in the Presence of Dopamine and Pyridoxine Facilitated by their Extent of Interaction with Single‐walled Carbon Nanotubes

et al. 2019

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Single‐walled carbon nanotubes (SWCNTs) were immobilized on glassy carbon (GC) electrode by drop casting The resulting modified electrode (represented as GC/SWCNTs) efficiently oxidizes acetaminophen (AC), dopamine (DA) and pyridoxine (PY) by decreasing the respective oxidation potentials and increasing peak currents in comparison to bare GC electrode. The extent of lowering of overpotentials is in the order of AC>PY>DA, in agreement with the order of decrease in the HOMO‐LUMO energy gap (ΔE) of these analytes, as determined from Density Functional Theory (DFT) calculations. DFT calculations further reveal that due to the interaction of the analytes on the SWCNT(10,10) there is a negative charge density transfer (higher probability of electron transfer, lower ΔE value) to the frontier molecular orbitals of the analytes, which eases their oxidation. Since AC, DA and PY oxidize distinctly at distinct potential values, the present SWCNTs modified electrodes could be used to simultaneously determine them. Cyclic voltammetry, differential pulse voltammetry and amperometry techniques are utilized to understand the electrochemical characteristics of the analytes (AC, DA and PY) and subsequent sensing of them at the GC/SWCNTs electrode. The electrode is then applied to the determination of AC as a case study. Sensitivity, detection limit and linear calibration range for the AC are found to be 7.9 μA μM−1 cm−2, 1.1 μM and 2.0–100.0 μM, respectively. The increased electroactive surface area of the GC/SWCNTs increases the oxidation peak currents and hence increases the sensitivity of the determination.

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Electrochemical Sensor Based on Multi‐walled Carbon Nanotubes and Cobalt Phthalocyanine Composite for Pyridoxine Determination

Porto

Silva

et al. 2019

Electroanalysis

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In this work, an electrochemical sensor based on pyrolytic graphite electrode (PGE), cobalt phthalocyanine (CoPc) and multiwalled carbon nanotube (MWCNT) composite designed as PGE‐MWCNT/CoPc was developed and validated for pyridoxine (vitamin B6) determination employing Differential Pulse Voltammetry (DPV). The electrochemical behaviour of pyridoxine at the PGE‐MWCNT/CoPc has been evaluated and the charge transfer coefficient, α, and the charge transfer rate constant, κ, were calculated as 0.30 and 11.67±0.43 s−1, respectively, which indicates that, although this system is irreversible, it is viable kinetically to be used as a sensor. The optimized experimental conditions were pH 5.5 in 0.30 mol L−1 phosphate buffer. The linear range found was 10 to 400 μmol L−1 of pyridoxine, with r=0.9987. The limits of detection and quantification were 0.50 and 1.67 μmol L−1, respectively, showing the good sensitivity of the method. The method was successfully applied for the pyridoxine determination in real samples of pharmaceutical formulation with RSD% lower than 5 % indicating that it can be used for routine quality control pharmaceutical formulations containing pyridoxine. Furthermore, it has the advantages of a fast response, a low detection limit and low cost.

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Highly sensitive interference-free electrochemical determination of pyridoxine at graphene modified electrode: Importance in Parkinson and Asthma treatments

Cited by 27 publications

References 39 publications

Cost‐effective and Facile Production of a Phosphorus‐doped Graphite Electrode for the Electrochemical Determination of Pyridoxine

Cost‐effective and Facile Production of a Phosphorus‐doped Graphite Electrode for the Electrochemical Determination of Pyridoxine

Sensitive Determination of Acetaminophen in the Presence of Dopamine and Pyridoxine Facilitated by their Extent of Interaction with Single‐walled Carbon Nanotubes

Electrochemical Sensor Based on Multi‐walled Carbon Nanotubes and Cobalt Phthalocyanine Composite for Pyridoxine Determination

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