Highly sensitive H2O2 sensor based on poly(azure A)-platinum nanoparticles deposited on activated screen printed carbon electrodes

Abstract

The sensitive determination of hydrogen peroxide has broad analytical applications. In this work, a novel non-enzymatic hydrogen peroxide sensor based on Pt nanoparticles (PtNPs) electrochemically deposited on previously modified and activated screen-printed carbon electrodes (aSPCEs) was constructed. The pretreatment consisted of subjecting the electrodes to a surface activation treatment with hydrogen peroxide followed by the electrodeposition of poly(azure A) films (PAA) in a sodium dodecyl sulfate micellar aqueous solution. The PtNPs/PAA/aSPCEs were characterized by scanning electron microscope, X-Ray photoelectron spectrometry, linear scan voltammetry and electrochemical impedance spectroscopy. Linear sweep voltammograms showed that the oxidation peak potential of H2O2 shifts from ~1 V at SPCEs to ~0.1 V at PtNPs/PAA/aSPCEs. The fabricated electrodes showed excellent electrocatalytic activity towards H2O2 oxidation, making its detection possible at 0.1 V. The detection limit was 51.6 nM, which is significantly lower than other modified electrodes found in the literature, and the linear range ranging from 0 to 300 µM. The proposed electrode was successfully applied to the determination of H2O2 in real samples in different areas. Additional experiments against common interfering agents (ascorbic acid, dehydroascorbic acid, glucose, salicylic acid, among other compounds) showed no increase in the current signal and only in the case of ascorbic acid a small interference, not greater than 10% is observed, which indicates high specificity of the sensor. These electrodes open up alternative avenues for the development of highly sensitive, robust and low cost electrochemical H2O2 sensors for field tests.

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

“…This major content in the oxygen-containing functional groups might positively influence the anchorage of the polymer to the working electrode of the aSPCE. Furthermore, the N1s analysis revealed a higher amount (at.%) of nitrogen in PAA-aSPCE ( Figure 4 B), which could be indicative of a thicker electrodeposited PAA film on the activated carbonaceous surface, with the corresponding increase in the available surface for PtNPs deposition [ 29 ]. In addition, the nitrogen species found in PAA-aSPCE are different to those in PAA-SPCE.…”

“…In particular, an H 2 O 2 -basedpre-treatment (carbon activation) [ 25 , 26 ], the polymerization of poly(Azure A) (PAA) using dodecyl sulphate (DS) as a doping ion [ 19 , 27 ], and the electrodeposition of platinum nanoparticles (PtNPs) [ 18 , 28 ] on screen-printed carbon electrodes (SPCEs) have demonstrated excellent electrochemical properties in the measurement of H 2 O 2 . Recently, our research group published a novel non-enzymatic H 2 O 2 sensor based on PtNPs electrochemically deposited on a PAA film previously electrogenerated on screen-printed carbon electrodes whose surface was previously activated by H 2 O 2 (aSPCEs) [ 29 ]. The synergy between PAA and PtNPs and the high electrochemical surface obtained allowed for the development of a highly sensitive H 2 O 2 sensor at a low overpotential, with a very appropriate limit of detection, stability, and reproducibility.…”

Electrochemical Detection of H2O2 Released from Prostate Cancer Cells Using Pt Nanoparticle-Decorated rGO–CNT Nanocomposite-Modified Screen-Printed Carbon Electrodes

“…As shown in Figure 3b, the cathodic peak current (I pc ) and peak potential (E pc ) of PtNP/SPCE were −112.9 µA and −0.36 V, respectively. After deposition of rGO-CNT on PtNP/SPCE, the I pc decreased to −83.2 µA and the E pc increased to −0.47 V. Because PtNPs possessed better electrocatalytic property toward H 2 O 2 [27], the I pc of rGO-CNT/PtNP/SPCE was lower than that of PtNP/SPCE, and the E pc of rGO-CNT/PtNP/SPCE was shifted to a more negative potential due to slow electron transfer kinetics between rGO-CNT nanocomposite and H 2 O 2 , compared to PtNP/SPCE. This result indicates that the rGO-CNT nanocomposite was successfully deposited onto the surface of PtNP/SPCE.…”

“…In addition, different electrochemical behavior of PtNP/SPCE and PtNP/rGO-CNT/PtNP/SPCE can be explained by the PtNP dispersion and surface properties. The interconnected structure of the rGO-CNT nanocomposite can facilitate the dispersion of PtNPs on their surface, increasing the specific area of these materials, and it can improve the catalytic efficiency [27,47]. Furthermore, owing to the high electronic conductivity of the rGO-CNT nanocomposite, charge might be easily transmitted along the rGO-CNT networks toward the dispersed PtNPs where the electrocatalytic reaction occurs [27].…”

“…To solve this problem, non-enzymatic sensors based on noble metal nanoparticles (NPs), such as gold (Au), silver (Ag), platinum (Pt), and Pt/Au bimetallic nanostructure, have received much more attention due to their unique advantages, such as rapid response and high stability [22][23][24][25][26]. Among them, PtNPs exhibit good electrocatalytic property toward H 2 O 2 , as well as convenience of electron transfer and biocompatibility [27]. It has been demonstrated that they decrease the oxidation/reduction overvoltage in the determination of H 2 O 2 , which can easily avoid the interferents, like ascorbic acid (AA) and uric acid (UA) [28,29].…”