Highly-sensitive graphene field effect transistor biosensor using PNA and DNA probes for RNA detection

Tian, Meng; Qiao, Ming; Shen, Congcong; Meng, Fanlu; Frank, Ludmila A.; Krasitskaya, Vasilisa V.; Wang, Zhihui; Zhang, Xiumei; Song, Ruihong; Li, Yingxian; Liu, Jianjian; Xu, Shicai; Wang, Jihua

doi:10.1016/j.apsusc.2020.146839

Cited by 60 publications

(36 citation statements)

References 55 publications

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“…Different experiments show that FET based on graphene functionalized with PBASE have high conductivity and sensitivity. Functionalization with PBASE neither induces defects in the AGNR, nor changes the electrical and physical properties of AGNR (Xu et al 2017 ; Ping et al 2016 ; Kim et al 2020 ; Vishnubhotla et al 2020 ; Béraud et al 2021 ; Hao et al 2020 ; Fernandes et al 2019 ; Tsang et al 2019 ; Mudusu et al 2020 ; Xia et al 2021 ; Tian et al 2020 , 2018 ; Ohno et al 2013 ; Guo et al 2011 ; Wu et al 2015 , 2017 ; Gao et al 2018 ; Forsyth et al 2017 ). Based on these results, we have functionalized AGNR with PBASE.…”

Section: Simulation Details and Methodsmentioning

confidence: 99%

Real-time label-free detection of DNA hybridization using a functionalized graphene field effect transistor: a theoretical study

Bagherzadeh-Nobari

Kalantarinejad²

2021

J Nanopart Res

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Graphical abstract Detection of DNA hybridization with high sensitivity and accuracy plays a major role in clinical diagnosis and treatment. Despite intense experimental studies of graphene field effect transistor as DNA hybridization detector, the mechanism of detection and changes in the electrical properties of the device is not investigated in detail. To this end, we have investigated an armchair graphene nanoribbon (AGNR) interconnected between gold electrodes as a detector of DNA hybridization. Using non-equilibrium Green’s function method and density functional theory, the effect of 1-pyrenebutanoic acid succinimidyl ester (PBASE) linker, probe, and target DNA on the electrical properties of the device has been investigated at zero bias voltage. The results show that, after functionalization of AGNR with PBASE, the conductance of the device increases while functionalization with probe and target DNA leads to a decrease in conductance. The changes in the projected density of states on the AGNR and transmission around Fermi energy are the reason for the change in conductance of the system. In all cases, both charge transfer and electrostatic gating are responsible for the change in the electrical properties of the system. The results show that our device detects DNA hybridization with a sensitivity of 10% at zero bias voltage, and by applying a suitable gate voltage, it can show higher sensitivity.

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Section: Simulation Details and Methodsmentioning

confidence: 99%

Real-time label-free detection of DNA hybridization using a functionalized graphene field effect transistor: a theoretical study

Bagherzadeh-Nobari

Kalantarinejad²

2021

J Nanopart Res

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“…If the material used in the channel is graphene, it is called a graphene field-effect transistor (GFET) ( Prattis et al, 2021 ). For example, a peptide nucleic acid (PNA) based GFET quantitatively detected RNA target with the graphene ( Tian et al, 2020 ). The PNA probe with an amine bond frame provided specific binding efficiency to DNA and RNA targets, thus reducing the detection time ( Mat Zaid et al, 2017 ; Movilli et al, 2018 ).…”

Section: Intelligent Wearable Sensorsmentioning

confidence: 99%

Recent Progress in Intelligent Wearable Sensors for Health Monitoring and Wound Healing Based on Biofluids

Cheng

Zhou

et al. 2021

Front. Bioeng. Biotechnol.

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The intelligent wearable sensors promote the transformation of the health care from a traditional hospital-centered model to a personal portable device-centered model. There is an urgent need of real-time, multi-functional, and personalized monitoring of various biochemical target substances and signals based on the intelligent wearable sensors for health monitoring, especially wound healing. Under this background, this review article first reviews the outstanding progress in the development of intelligent, wearable sensors designed for continuous, real-time analysis, and monitoring of sweat, blood, interstitial fluid, tears, wound fluid, etc. Second, this paper reports the advanced status of intelligent wound monitoring sensors designed for wound diagnosis and treatment. The paper highlights some smart sensors to monitor target analytes in various wounds. Finally, this paper makes conservative recommendations regarding future development of intelligent wearable sensors.

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“…The advantage of PNA is that it provides a great advantage in reducing the determination time. Tian et al [21] used graphene field-effected transistors in their PNA-modified electrochemical biosensor study. In the DNA analysis study by measuring the gate voltage, it reached the level of 0.1 aM LOD and performed between 0.1 aM and 1 pM.…”

Section: Dna and Rna Usage In Biosensor Technologymentioning

confidence: 99%

Nucleic Acids for Electrochemical Biosensor Technology

Uygun¹,

Uygun²,

Sağın³

2021

Biosensors - Current and Novel Strategies for Biosensing

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Biosensor technology has developed extremely rapidly in recent years. This technology brings along precise measurements as well as specific measurements. Thanks to its ability to be miniaturized and be easily accessible to the end user, it is one-step ahead of other similar methods. The selectivity of biological molecules and the sensitivity of electrochemical methods enable the continuous evolvement of these new technologies. In this chapter, the use of nucleic acids as both recognition agents and target molecules, the way they are used in biosensor technology and their electrical properties are explained in detail with examples. Aptamers, which are synthetic nucleic acids, and their use in electrochemical biosensor systems with different electrochemical and immobilization methods have been compared extensively.

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Highly-sensitive graphene field effect transistor biosensor using PNA and DNA probes for RNA detection

Cited by 60 publications

References 55 publications

Real-time label-free detection of DNA hybridization using a functionalized graphene field effect transistor: a theoretical study

Real-time label-free detection of DNA hybridization using a functionalized graphene field effect transistor: a theoretical study

Recent Progress in Intelligent Wearable Sensors for Health Monitoring and Wound Healing Based on Biofluids

Nucleic Acids for Electrochemical Biosensor Technology

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