Highly Sensitive Enzymatic Biosensor Based on Polyaniline-Wrapped Titanium Dioxide Nanohybrid for Fish Freshness Detection

Thakur, Deeksha; Pandey, Chandra Mouli; Kumar, Devendra

doi:10.1007/s12010-022-03931-7

Cited by 17 publications

(7 citation statements)

References 41 publications

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“…An easy method suggested by Watanabe et al has been used to extract Xn from Rohu ( Labeo rohita ) fish and prepare the Xn solution. 21 The DPV study shows that the peak current steadily increases as the concentration of Xn increases from 0.001 to 1 μM. These results were identical to those of the synthetic Xn samples.…”

Section: Resultssupporting

confidence: 69%

“…The developed P o PD@g-C 3 N 4 nanocomposite-based sensor has been tested for Xn in the fish meat samples using DPV. An easy method suggested by Watanabe et al has been used to extract Xn from Rohu (Labeo rohita) fish and prepare the Xn solution . The DPV study shows that the peak current steadily increases as the concentration of Xn increases from 0.001 to 1 μM.…”

Section: Resultsmentioning

confidence: 99%

“…Nowadays, electrochemical techniques gained interest as being simple, sensitive, reliable, and affordable to use. 20 , 21 From the last decade, for hypoxanthine or xanthine detection, various conducting polymer-based nanocomposite biosensors are of interest such as polypyrrole-polyvinyl sulfonate (PPy-PTS) films, 22 polypyrrole- para -toluenesulfonate (PPy-pTS), 23 disposable amperometric biosensor with a screen-printed electrode, gold nanoparticles with single-walled carbon nano-horn (GNPs/SWCNH), 24 polymeric mediator/multiwalled carbon nanotube (MWCNT) 25 reduced graphene oxide/iron oxide bio-nanocomposite interface, 26 polymerized 10-[4 H -dithieno (3,2- b : 2′,3′- d ) pyrrole-4-yl] decane-1-amine film, 27 and so forth. However, these biosensors show a higher limit of detection, and the linear range may be due to slow electron transfer, poor stability, reusability, fragility, and poor absorption ability, which created requirement for introducing a system with a highly desirable lower detection limit and fast electron transfer.…”

Section: Introductionmentioning

confidence: 99%

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Graphitic Carbon Nitride-Wrapped Metal-free PoPD-Based Biosensor for Xanthine Detection

2022

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A metal-free, enzymatic biosensor was developed using graphitic carbon nitride (g-C3N4)-wrapped poly-ortho-phenylenediamine (PoPD) for the determination of xanthine (Xn). Field emission scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction confirmed the successful formation of the PoPD, g-C3N4 nanosheets and PoPD@g-C3N4 nanocomposite. Furthermore, the electrochemical behavior of the biosensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The prepared enzyme electrode exhibited maximum response at pH 7.5 with a response time of 5 s, and its sensitivity was 5.798 μAM–1. The nanocomposite shows exceptional sensing capabilities for detecting Xn, having a wide linear range from 1 nM to 1 μM with a relatively low detection limit of 0.001 nM. The biosensor shows good stability (4 weeks) and reproducibility and can detect the presence of Xn from other interfering analytes. Validation of the biosensor with real samples obtained from Rohu (Labeo rohita) fish shows that the fabricated biosensor has the requisite potential to be used for Xn detection in meat samples.

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Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Graphitic Carbon Nitride-Wrapped Metal-free PoPD-Based Biosensor for Xanthine Detection

2022

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“…In addition to the metallic nanocomposite, other materials, such as organic materials, are also being incorporated with metallic nanomaterials to develop enzymatic nanobiosensors. In one study, polyaniline (PANI), a conducting polymer, was combined with titanium dioxide (PANI@TiO 2 ) and prepared on an indium tin oxide (ITO) electrode using electrophoretic deposition to provide enhanced electron transfer kinetics for xanthine oxidase (XOs) immobilized on the PANI@TiO 2 electrode [ 82 ]. Using the enzymatic activity of XOs for the detection of xanthine (Xn), Xn was detected sensitively with a 100 nM LoD and rapid response (within 10 s) using the DPV technique.…”

Section: Enzymatic Electrochemical Nanobiosensorsmentioning

confidence: 99%

Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals

Choi

Yoon

2023

Biosensors

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The detection of small molecules has attracted enormous interest in various fields, including the chemical, biological, and healthcare fields. In order to achieve such detection with high accuracy, up to now, various types of biosensors have been developed. Among those biosensors, enzymatic biosensors have shown excellent sensing performances via their highly specific enzymatic reactions with small chemical molecules. As techniques used to implement the sensing function of such enzymatic biosensors, electrochemical and fluorescence techniques have been mostly used for the detection of small molecules because of their advantages. In addition, through the incorporation of nanotechnologies, the detection property of each technique-based enzymatic nanobiosensors can be improved to measure harmful or important small molecules accurately. This review provides interdisciplinary information related to developing enzymatic nanobiosensors for small molecule detection, such as widely used enzymes, target small molecules, and electrochemical/fluorescence techniques. We expect that this review will provide a broad perspective and well-organized roadmap to develop novel electrochemical and fluorescent enzymatic nanobiosensors.

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“…Meat consumption is rising among people and has become crucial to a balanced diet for humans since it is a great source of proteins and vitamins [ 1 ]. Meat quality, however, starts deteriorating immediately upon the death of the animal.…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Electrochemical Sensor for Rapid and Selective Detection of Hypoxanthine

Garg

Verma

Monika³

2022

Biosensors

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In this paper, we report on the coupling of an electrochemical transducer with a specifically designed biomimetic and synthetic polymeric layer that serves as a recognition surface that demonstrates the molecular memory necessary to facilitate the stable and selective identification of the meat-freshness indicator hypoxanthine. Consumer preferences and the food safety of meat products are largely influenced by their freshness, so it is crucial to monitor it so as to quickly identify when it deteriorates. The sensor consists of a glassy-carbon electrode, which can be regenerated in situ continuously, functionalized with molecularly imprinted polymers (MIPs) and a nanocomposite of curcumin-coated iron oxide magnetic nanospheres (C-IO-MNSs) and multiwalled carbon nanotubes (MWCNTs) that enhance the surface area as well as the electroactive characteristics. The electrochemical behavior of the fabricated sensor was analyzed by both cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Differential pulse voltammetric studies revealed the rapid response of the proposed sol-gel-MIP/MWCNT/C-IO-MNS/GCE sensor to hypoxanthine in a concentration range of 2–50 µg/mL with a lower limit of detection at 0.165 μg/mL. Application of the newly fabricated sensor demonstrated acceptable recoveries and satisfactory accuracy when used to measure hypoxanthine in different meat samples.

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Highly Sensitive Enzymatic Biosensor Based on Polyaniline-Wrapped Titanium Dioxide Nanohybrid for Fish Freshness Detection

Cited by 17 publications

References 41 publications

Graphitic Carbon Nitride-Wrapped Metal-free PoPD-Based Biosensor for Xanthine Detection

Graphitic Carbon Nitride-Wrapped Metal-free PoPD-Based Biosensor for Xanthine Detection

Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals

Molecularly Imprinted Polymer-Based Electrochemical Sensor for Rapid and Selective Detection of Hypoxanthine

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