Highly sensitive determination of recombinant human erythropoietin-α in aptamer-based affinity probe capillary electrophoresis with laser-induced fluorescence detection

Shen, Rui; Guo, Lei; Zhang, Zhaoyang; Meng, Qingwei; Xie, Jianwei

doi:10.1016/j.chroma.2010.06.072

Cited by 35 publications

(19 citation statements)

References 30 publications

(44 reference statements)

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“…Some detection methods based on this anti-rHuEPO-α aptamer have been developed and proved its feasibility [24–26]. …”

Section: Resultsmentioning

confidence: 99%

A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α

Sun¹,

Guo

Zhang³

et al. 2011

Sensors

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We present here a novel conjugated aptamer-gold nanoparticle (Apt-AuNPs) fluorescent probe and its application for specific detection of recombinant human erythropoietin-α (rHuEPO-α). In this nanobiosensor, 12 nm AuNPs function as both a nano-scaffold and a nano-quencher (fluorescent energy acceptor), on the surface of which the complementary sequences are linked (as cODN-AuNPs) and pre-hybridized with carboxymethylfluorescein (FAM)-labeled anti-rHuEPO-α aptamers. Upon target protein binding, the aptamers can be released from the AuNP surface and the fluorescence signal is restored. Key variables such as the length of linker, the hybridization site and length have been designed and optimized. Full performance evaluation including sensitivity, linear range and interference substances are also described. This nanobiosensor provides a promising approach for a simple and direct quantification of rHuEPO-α concentrations as low as 0.92 nM within a few hours.

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“…Some detection methods based on this anti-rHuEPO-α aptamer have been developed and proved its feasibility [24–26]. …”

Section: Resultsmentioning

confidence: 99%

A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α

Sun¹,

Guo

Zhang³

et al. 2011

Sensors

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“…In another work Shen et al used affinity probe capillary electrophoresis coupled with LIF to study the interaction of recombinant erythropoietin-a (used in treatment of anemia) and fluorescently labeled specific ssDNA aptamer [47].…”

Section: Protein-dna Interactionsmentioning

confidence: 99%

Recent Advances in Affinity Capillary Electrophoresis for Binding Studies

et al. 2014

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The present review covers recent advances and important applications of affinity capillary electrophoresis (ACE). It provides an overview about various ACE types, including ACE-MS, the multiple injection mode, the use of microchips and field-amplified sample injection-ACE. The most common scenarios of the studied affinity interactions are protein-drug, protein-metal ion, protein-protein, protein-DNA, protein-carbohydrate, carbohydrate-drug, peptide-peptide, DNA-drug and antigen-antibody. Approaches for the improvements of ACE in term of precision, rinsing protocols and sensitivity are discussed. The combined use of computer simulation programs to support data evaluation is presented. In conclusion, the performance of ACE is compared with other techniques such as equilibrium dialysis, parallel artificial membrane permeability assay, high-performance affinity chromatography as well as surface plasmon resonance, ultraviolet, circular dichroism, nuclear magnetic resonance, Fourier transform infrared, fluorescence, MS and isothermal titration calorimetry.

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“…The method was successfully applied for the quantification of rHuEPO‐α in physiological buffer, artificial urine and human serum. The linear range for rHuEPO‐α was from 0.2 to 100 nM and the limit of detection was 0.2 nM 47.…”

Section: Proteins and Peptides Of Forensic Interestmentioning

confidence: 99%

Recent advances in the application of CE to forensic sciences, an update over years 2009–2011

2011

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The present article reviews and comments the applications of capillary electrophoresis in the different areas of forensic sciences covering the time from the second half of 2009 until the first part of 2011, being the latest update of previous reviews covering the years from 2001 to 2009. Numerous articles reporting applications of capillary electrophoresis to analytical problems of potential interest for the forensic researchers and scientists can be found in the most qualified journals of analytical chemistry, analytical biochemistry, pharmacology, toxicology, laboratory medicine, human genetics, etc. However, the present review has been focused on discussing only the most relevant examples of analytical applications of capillary electrophoretic and electrokinetic techniques published in the following fields: (i) illicit and abused drugs, (ii) ions and small molecules of forensic interest, (iii) proteins and peptides of forensic interest, (iv) dyes and inks, (v) forensic DNA. The present review collects and comments on 60 references.

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Highly sensitive determination of recombinant human erythropoietin-α in aptamer-based affinity probe capillary electrophoresis with laser-induced fluorescence detection

Cited by 35 publications

References 30 publications

A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α

A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α

Recent Advances in Affinity Capillary Electrophoresis for Binding Studies

Recent advances in the application of CE to forensic sciences, an update over years 2009–2011

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