A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α

Sun, Jingbo; Guo, Aitao; Zhang, Zhaoyang; Guo, Lei; Xie, Jianwei

doi:10.3390/s111110490

Cited by 31 publications

(9 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wang et al [19] reported a FRET-based aptasensor of Ochratoxin A (OTA) when fluorescein amidite (FAM) and gold nanoparticles (AuNPs) were as energy donor and acceptor pair, respectively, and the detection limit was about 2 × 10 −12 g/mL. Sun et al [20] presented a FRET-based aptasensor of recombinant human erythropoietin-α (rHuEPO-α) when AuNPs were as the energy donors, and the detection limit was about 0.92 × 10 −9 mol/L.…”

Section: Introductionmentioning

confidence: 99%

Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Meng

Zhao

Guo

et al. 2016

Nanomaterials and Nanotechnology

View full text Add to dashboard Cite

A fluorescence resonance energy transfer (FRET)-based aptamer biosensor (aptasensor) for sensitive determination of adenosine triphosphate (ATP) was developed. The energy donor and acceptor were 5′-carboxyfluorescein (5′-FAM) and gold nanoparticles (AuNPs), respectively. AuNPs were linked to 3′-SH-DNA by Au-S bond. This aptasensor was obtained by the hybridization between the complementary DNA strands of the 5′-FAM and AuNPs. The detecting results indicated that the FRET increase efficiency (F r) of detecting system at 519 nm was linear when the concentration of ATP target was about 0.1-10 mmol/L. Moreover, the aptasensors showed a good stability in different buffer conditions and a low-detection limit (15.2 nmol/L) for ATP.

show abstract

Section: Introductionmentioning

confidence: 99%

Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Meng

Zhao

Guo

et al. 2016

Nanomaterials and Nanotechnology

View full text Add to dashboard Cite

show abstract

“…Since AuNPs are known as strong quenchers Sun, Guo, Zhang, Guo, & Xie, 2011) fluorescent resonance energy transfer (FRET). In the absence of streptomycin a strong fluorescence emission is observed.…”

Section: Sensing Schemementioning

confidence: 99%

Colorimetric and fluorescence quenching aptasensors for detection of streptomycin in blood serum and milk based on double-stranded DNA and gold nanoparticles

et al. 2016

View full text Add to dashboard Cite

“…Aptamers are single-stranded RNA or DNA molecules that have been selected to bind specifically for a given protein or small molecule analyte . The versatility of aptamers as recognition elements in affinity sensors has seen them attract widespread interest as alternatives to antibodies. − In modern biosensing, aptamers are often combined with nanoparticles . Despite the wide application of aptamer–nanoparticle conjugates reported in the literature, an understanding of how the surface architecture of the aptamer-modified nanoparticle influences the binding interaction with the target analyte, and hence the final biosensor performance, is still limited.…”

mentioning

confidence: 99%

Impact of the Coverage of Aptamers on a Nanoparticle on the Binding Equilibrium and Kinetics between Aptamer and Protein

et al. 2020

View full text Add to dashboard Cite

Knowledge of the interaction between aptamer and protein is integral to the design and development of aptamer-based biosensors. Nanoparticles functionalized with aptamers are commonly used in these kinds of sensors. As such, studies into how the number of aptamers on the nanoparticle surface influence both kinetics and thermodynamics of the binding interaction are required. In this study, aptamers specific for interferon gamma (IFN-γ) were immobilized on the surface of gold nanoparticles (AuNPs), and the effect of surface coverage of aptamer on the binding interaction with its target was investigated using fluorescence spectroscopy. The number of aptamers were adjusted from an average of 9.6 to 258 per particle. The binding isotherm between AuNPs–aptamer conjugate and protein was modeled with the Hill-Langmuir equation, and the determined equilibrium dissociation constant (K′D) decreased 10-fold when increasing the coverage of aptamer. The kinetics of the reaction as a function of coverage of aptamer were also investigated, including the association rate constant (k on) and the dissociation rate constant (k off). The AuNPs–aptamer conjugate with 258 aptamers per particle had the highest k on, while the k off was similar for AuNPs–aptamer conjugates with different surface coverages. Therefore, the surface coverage of aptamers on AuNPs affects both the thermodynamics and the kinetics of the binding. The AuNPs–aptamer conjugate with the highest surface coverage is the most favorable in biosensors considering the limit of detection, sensitivity, and response time of the assay. These findings deepen our understanding of the interaction between aptamer and target protein on the particle surface, which is important to both improve the scientific design and increase the application of aptamer–nanoparticle based biosensor.

show abstract

A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α

Cited by 31 publications

References 30 publications

Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate

Colorimetric and fluorescence quenching aptasensors for detection of streptomycin in blood serum and milk based on double-stranded DNA and gold nanoparticles

Impact of the Coverage of Aptamers on a Nanoparticle on the Binding Equilibrium and Kinetics between Aptamer and Protein

Contact Info

Product

Resources

About