Highly sensitive detection of rabbit IgG by electron spin resonance using CuS nanoparticles as probe

Tian, Sizhu; Li, Xuwen; Jiang, Jia; Tang, Li; Zhang, Hanqi; Yu, Yong; Zhang, Ziwei

doi:10.1016/j.snb.2021.129835

Cited by 6 publications

(2 citation statements)

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“…20 Since the ESR technique has some unique and excellent performances, such as high measurement sensitivity, a small amount of sample required, and no damage to the detection sample, 21,22 it has been widely applied in many fields such as food science, biology, physics, chemistry, and medicine. 23 Herein, we developed a method to quantitatively detect BChE and OPs in plant samples based on directly measuring Mn 2+ by ESR spectroscopy. In this strategy, MnO 2 nanosheets with no ESR signal are reduced into Mn 2+ by thiocholine generated from the reaction catalyzed by BChE.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The research objects of ESR spectroscopy include free radicals, transition/rare-earth metal ions and their compounds, as well as impurities and defects in solids . Since the ESR technique has some unique and excellent performances, such as high measurement sensitivity, a small amount of sample required, and no damage to the detection sample, , it has been widely applied in many fields such as food science, biology, physics, chemistry, and medicine …”

Section: Introductionmentioning

confidence: 99%

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Label-Free and Ultrasensitive Detection of Butyrylcholinesterase and Organophosphorus Pesticides by Mn(II)-Based Electron Spin Resonance Spectroscopy with a Zero Background Signal

et al. 2022

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Mn(II)-based electron spin resonance (ESR) spectroscopy was used for detecting butyrylcholinesterase (BChE) and organophosphorus pesticides (OPs). MnO2 nanosheets were synthesized with manganese chloride and hydrogen peroxide. With the catalysis of BChE, S-butyrylthiocholine iodide (BTCh) was hydrolyzed into thiocholine which has a reducing −SH group. In the presence of thiocholine, MnO2 nanosheets were broken down and Mn(IV) in MnO2 nanosheets was reduced into Mn(II). Mn2+ is a paramagnetic ion and gives a good ESR signal. In contrast, MnO2 nanosheets have no ESR signal and need not be separated from Mn2+. Mn2+ can be determined directly by ESR spectroscopy, and no further sensing probe is needed. ESR spectroscopy based on directly detecting Mn2+ is much simpler than those using other probes besides MnO2. The ESR signal of Mn2+ is proportional to the catalytic activity of BChE. OPs which inhibit the activity of BChE can also be detected by probing the ESR signal of Mn2+. Since there is no ESR signal of MnO2 nanosheets, the background signal in the absence of BChE was close to zero. The limit of detection (LOD) of BChE was as low as 0.042 U L–1. The standard curve for determining the OP paraoxon was established by measuring the inhibition of BChE by paraoxon, and the LOD of paraoxon was found to be 0.076 ng mL–1. The spiked Chinese cabbage extract samples were analyzed, and the experimental results indicated that the recoveries were from 96.5 to 102.8%. The planted Chinese cabbage was sprayed with the paraoxon solution, and the residue amount of paraoxon in the extract was estimated by the method. The result obtained by the present method was consistent with that obtained by HPLC, which proved the practicability of this new method.

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