Highly sensitive detection of cancer antigen human epidermal growth factor receptor 2 using novel chicken egg yolk immunoglobulin

Sun, Yong; Yang, Yiheng; Wang, Lifen; Lv, Li; Zhu, Jie; Han, Wenqi; Wang, Enxia; Guo, Xin; Zhen, Yuhong

doi:10.1016/j.biologicals.2015.03.002

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…Anti-HER2-A and anti-HER2-B IgY effectively detected the HER2 glycoprotein in cultured breast cancer SK-BR-3 cells by immunofluorescence and in sections of breast tumors over expressing HER2-B, using immunohistochemistry. In addition, the antibodies bound to the glycoprotein in ELISA and Western blot, which indicates that IgY against HER2 is promising for use in breast cancer diagnosis [117].…”

Section: Diagnosis Of Tumorsmentioning

confidence: 96%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

124

135

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A B S T R A C TEgg yolk constitutes a relevant alternative source of antibodies. It presents some advantages over mammalian serum immunoglobulins regarding productivity, animal welfare and specificity. The main immunoglobulin present in avian blood (IgY) is transmitted to their offspring and accumulates in egg yolks, which enables the non-invasive harvesting of high amounts of antibodies. Moreover, due to structural differences and phylogenetic distance, IgY is more suitable for diagnostic purposes than mammalian antibodies, since it does not react with certain components of the human immune system and displays greater avidity for mammalian conserved proteins. IgY has been extensively used in health researches, as both therapeutic and diagnostic tool. This article aims to review its applications in both human and veterinary health.

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Section: Diagnosis Of Tumorsmentioning

confidence: 96%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

124

135

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“…Ab-B or Ab-C were cultured in Tryptic Soy Broth (TSB) media for 24 h and collected in PBS at a concentration of 10 9 cfu/ ml and then inactivated with 0.7% (w/v) formaldehyde for 24 h. The inactivated antigen suspensions were emulsified with an equal volume of complete and incomplete Freund's adjuvant for the first and subsequent booster immunizations, respectively. Hens were immunized according to reported methods [24]. Fifteen Hy-line laying hens were divided into three groups and immunized with the two vaccines and PBS, respectively.…”

Section: Production Of Igys Against Pdr-abmentioning

confidence: 99%

Effects of specific egg yolk immunoglobulin on pan-drug-resistant Acinetobacter baumannii

Shi

Zhu

Zou

et al. 2017

Biomedicine & Pharmacotherapy

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With the growing emergence of pan-drug-resistant Acinetobacter baumannii (PDR-Ab) strains in clinical, new strategies for the treatment of PDR-Ab infections are urgently needed. Egg yolk immunoglobulin (IgY) as a convenient and inexpensive antibody has been widely applied to the therapy of infectious diseases. The aim of this study was to produce IgY specific to PDR-Ab and investigate its antibacterial effects in vitro and in vivo. IgYs specific to two PDR-Ab strains were produced by immunizing hens with formaldehyde inactivated PDR-Ab cells and isolated from yolks with a purity of 90% by water dilution, salt precipitations and ultrafiltration. IgYs showed high titers when subjected to an ELISA and inhibited the growth of PDR-Ab in a dose-dependent manner in liquid medium. Scanning electron microscopy assay showed structural modification and aggregation of PDR-Ab treated with specific IgYs. Freshly cultured PDR-Ab cells were nasally inhaled in BALB/c mice to induce acute pneumonia. The infected mice were intraperitoneally injected with specific IgYs using cefoperazone/sulbactam and dexamethasone as positive controls. The IgYs specific to PDR-Ab lowered the mortality of mice with PDR-Ab-induced acute pneumonia, decreased the level of TNF-α and IL-1β in serum and reduced inflammation in lung tissue. Specific IgY has the potential to be used as a new therapeutic approach for the treatment of A. baumannii-induced infections.

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“…in parallel, the immunoglobulin y strategy to produce antibodies against cancerous biomarkers has been extensively used to develop tools (eliSA/immunohistochemistry) that contribute to the diagnosis/prognosis of the disease. [123][124][125][126][127][128][129][130]…”

Section: Egg Proteins In Targeting Cancer Cellsmentioning

confidence: 99%

Bioactive Minor Egg Components

Gautron

Guyot

Brionne

et al. 2019

Eggs as Functional Foods and Nutraceuticals for Human Health

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In the last 15 years, the development of functional genomics has increased the number of egg proteins identified from 50 to about 1300. These proteins are initially present in eggs to support a harmonious embryonic development. Consequently, this closed embryonic chamber contains molecules exhibiting diverse functions, including defense, nutrition and many predicted biological activities, which have been investigated using both bioinformatics and experimental investigations. In this chapter, we focus on some very interesting activities of high potential reported for minor egg proteins (excluding ovalbumin, ovotransferrin and lysozyme). The shell matrix proteins are involved in the calcification process to define and control the final texture of the shell and thereby its mechanical properties. Antimicrobial proteins are part of innate immunity and are mainly present in the white and vitelline membranes. They encompass several protein families, including protease inhibitors, vitamin-binding proteins, defensins, LBP-PLUNC family proteins and heparin-binding proteins. The egg also possesses additional bioactive proteins with direct anti-cancerous and antioxidant activities or whose biochemical properties are currently used to develop diagnostic tools and strategies for targeted therapy. Finally, this chapter also reports some emerging functions in tissue remodeling/wound healing and proposes some relevant bioactive candidates and research fields that would be interesting to investigate further.

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Highly sensitive detection of cancer antigen human epidermal growth factor receptor 2 using novel chicken egg yolk immunoglobulin

Cited by 4 publications

References 32 publications

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Effects of specific egg yolk immunoglobulin on pan-drug-resistant Acinetobacter baumannii

Bioactive Minor Egg Components

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