Highly sensitive cell imaging “Off–On” fluorescent probe for mitochondria and ATP

Srivastava, Priyanka; Razi, Syed S.; Ali, Rashid; Srivastav, Saurabh; Patnaik, Satyakam; Srikrishna, Saripella; Misra, Arvind

doi:10.1016/j.bios.2015.02.028

Cited by 56 publications

(39 citation statements)

References 33 publications

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“…First attempts to design ATP fluorescent probes were accented on the highly negative charge and stacking properties of the adenosine moiety of ATP . This approach has proven its efficiency over time and is still used for designing new receptors . To increase the stability of the probe–ATP complexes and decrease the detection limit, the principle of phosphate coordination with Zn 2+ ion was proposed and utilized in many ATP probe constructs (Figure S1‐A in the Supporting Information).…”

Section: Introductionmentioning

confidence: 99%

A Toolbox of Chromones and Quinolones for Measuring a Wide Range of ATP Concentrations

Pivovarenko

Bugera

Humbert

et al. 2017

Chemistry A European J

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A series of 26 3-hydroxychromones, three bis-flavonols and four 3-hydroxyquinolones were studied to evaluate their fluorescence response to interaction with ATP in buffer. The dyes differ by the total charge, the size and number of their aromatic units, as well as the position or electron-donating ability of their substituents. All of them were suggested to form complexes with ATP of 1:1 and 1:2 stoichiometry, which can be evidenced by their bright fluorescence and their 3000-6000 cm red-shifted excitation band. These fluorescent complexes allow detection of ATP concentrations over 3 orders of magnitude, whereas most other known probes cover no more than two orders. In total, the dyes allow ATP detection from 0.001 to 57 mm. In addition, most of the dye-ATP complexes can be excited in the visible and monitored in the red region of the spectrum. The response amplitude of the described dyes to ATP is as high as for the best known probes. Considering that complexation takes place at neutral pH, the studied dyes form a toolbox of fluorescent probes for intensiometric and ratiometric measurements of ATP concentration in a broad concentration range. Finally, the obtained results stimulate the idea that most of natural 3-hydroxyflavones in living cells may form complexes with ATP.

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Section: Introductionmentioning

confidence: 99%

A Toolbox of Chromones and Quinolones for Measuring a Wide Range of ATP Concentrations

Pivovarenko

Bugera

Humbert

et al. 2017

Chemistry A European J

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“…[9] A new type of ATP-selective probe, Rho-ATP-1, was disclosed in 2013, which utilized rhodamine lactam ring-opening to signal ATP binding. [10] An obvious advantage of the new sensing system is the turn-on type of fluorescence change through the ring opening process. Also, the ring-opening and -closing processes are reversible, allowing detection of dynamic ATP changes (Figure 4).…”

Section: Strategies To Develop Atp-selective Probesmentioning

confidence: 99%

“…Also, the ring-opening and -closing processes are reversible, allowing detection of dynamic ATP changes (Figure 4). [10][11] Rho-ATP-1, a lactam generated from diethylenetriamine, is structurally simple yet exhibited high selectivity toward ATP: only ATP caused the rhodamine lactam ring-opening and thus a large fluorescence change, whereas ADP and AMP, which have one and two less phosphates, caused little fluorescence change. In addition to the ionic or ionic hydrogen bonding interactions between the ATP phosphate group and the triamine in the protonated form, there seems to be p-p stacking interaction between the adenine base and the rhodamine core.…”

Section: Strategies To Develop Atp-selective Probesmentioning

confidence: 99%

Molecular Probes for Fluorescence Imaging of ATP in Cells and Tissues

et al. 2019

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Adenosine triphosphate (ATP), as the main energy source in cells, powers most cellular activities, such as synthesis and transportation of biomolecules, cell signaling, and muscle contraction. Therefore, detection and monitoring of ATP are essential to understand many biological processes, demanding molecular probes with high selectivity and sensitivity, fast response times, along with long‐term monitoring and tissue imaging capability. We overviewed the reported small‐molecule fluorescent probes for ATP by categorizing their recognition elements, along with comments on their merits and limitations. Also, we briefly stated the desirable properties, challenges, and perspectives in the development of ATP probes.

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“… ATP is hydrolyzed to ADP in a series of biological processes . Thus, those systems that have excellent ATP/ADP selectivity have been explored to detect the ratio of ATP/ADP concentrations in cells . Several publications have focused on the quantification of cAMP and ATP concentrations in human blood samples.…”

Section: Application Of Receptors and Probes For Nucleotidesmentioning

confidence: 99%

Molecular Receptors for Recognition and Sensing of Nucleotides

Agafontsev

Ravi

Shumilova

et al. 2018

Chemistry A European J

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Nucleotides are constituents of nucleic acids and they have a variety of functions in cellular metabolism. Synthetic receptors and sensors are required to reveal the role of nucleotides in living organisms and mechanisms of signal transduction events. In recent years, a large number of nucleotide‐selective synthetic receptors have been devised, which utilize different molecular designs and sensing mechanisms. This Minireview presents recent progress in the design of synthetic molecular receptors for selective recognition of nucleotides in aqueous solution. The binding properties of receptors and the origins of their selectivity for a particular nucleotide are discussed.

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Highly sensitive cell imaging “Off–On” fluorescent probe for mitochondria and ATP

Cited by 56 publications

References 33 publications

A Toolbox of Chromones and Quinolones for Measuring a Wide Range of ATP Concentrations

A Toolbox of Chromones and Quinolones for Measuring a Wide Range of ATP Concentrations

Molecular Probes for Fluorescence Imaging of ATP in Cells and Tissues

Molecular Receptors for Recognition and Sensing of Nucleotides

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