Highly sensitive and accurate detection of Vibrio cholera O1 OmpW gene by fluorescence DNA biosensor based on gold and magnetic nanoparticles

Narmani, Asghar; Kamali, Mohammad; Amini, Bahram; Kooshki, Hamid; Amini, Ali; Hasani, Leila

doi:10.1016/j.procbio.2017.10.009

Cited by 61 publications

(29 citation statements)

References 27 publications

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“…In the last decades, gold NPs (GNPs), due to their unique fluorescence, low cost, and so forth have been developed extensively to establish powerful optical methods for sensing and biomedical applications (Figure ; Narmani, Kamali, Amini, et al, ; Peng et al, ). Owing to higher atomic number and electron density, GNP fundamentally has a higher X‐ray absorption coefficient that makes it as suitable candidate for diagnostic and therapeutic applications.…”

Section: Metal‐ and Nonmetal‐based Nps In Drug Deliverymentioning

confidence: 99%

Folic acid functionalized nanoparticles as pharmaceutical carriers in drug delivery systems

Narmani

Rezvani

Farhood

et al. 2019

Drug Development Research

Self Cite

153

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Conventional chemotherapeutic approaches in cancer therapy such as surgery, chemotherapy, and radiotherapy have several disadvantages due to their nontargeted distributions in the whole body. On the other hand, nanoparticles (NPs) based therapies are remarkably progressing to solve several limitations of conventional drug delivery systems (DDSs) including nonspecific biodistribution and targeting, poor water solubility, weak bioavailability and biodegradability, low pharmacokinetic properties, and so forth. The enhanced permeability and retention effect escape from Pglycoprotein trap in cancer cells as a passive targeting mechanism, and active targeting strategies are also other most important advantages of NPs in cancer diagnosis and therapy. Folic acid (FA) is one of the biologic molecules which has been targeted overexpressed-folic acid receptor (FR) on the surface of cancer cells. Therefore, conjugation of FA to NPs most easily enhances the FR-mediated targeting delivery of therapeutic agents. Here, the recent works in FA which have been decorated NPs-based DDSs are discussed and cancer therapy potency of these NPs in clinical trials are presented. K E Y W O R D Scancer diagnosis and therapy, folic acid, nanoparticles, nanotechnology, targeted drug delivery

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Section: Metal‐ and Nonmetal‐based Nps In Drug Deliverymentioning

confidence: 99%

Folic acid functionalized nanoparticles as pharmaceutical carriers in drug delivery systems

Narmani

Rezvani

Farhood

et al. 2019

Drug Development Research

Self Cite

153

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“…Enzymes and gold nanoparticles are mostly used in colorimetric technique but the critical operating situation, high price and short lifetime prevent them from being used as a routine test in laboratories [13,14]. Paramagnetic nanoparticles and Silica nanoparticles with fast kinetics in solution, quick and easy separation by the magnet, excellent compatibility and ease of synthesis, low cost of production and the presence of a large hydrophilic surface, have more applicability [15]. In most studies, SiNPs have been used in white or colourless, which are not suitable for signal amplification and signal conversion [16].…”

Section: Introductionmentioning

confidence: 99%

Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Shams

Zarif

2019

Artificial Cells, Nanomedicine, and Biotechnology

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Brucellosis has always been a threat to the health and economics of societies. We report a new colorimetric immunoassay based on colored silica nanoparticles for detection of Brucella abortus. An immunosensor was designed based on blue-SiNPs and paramagnetic nanoparticles (PMNPs). The synthesized immunosensor was conjugated with a polyclonal antibody against B. abortus, which was activated by 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to form detection and capture probes, respectively. After adding the conjugates to the bacterial suspension, sandwich structure of PMNPs B. abortus-blue-SiNPs was formed and then separated by a magnet. The blue dye was released from the silica structure and its absorbance was measured at 670 nm with a spectrophotometer. Under optimal conditions, results showed a wide dynamic range from 1.5 Â 10 3 to 1.5 Â 10 8 cfu mL À1 with a detection limit of 450 cfu mL À1. The specificity of the sensor was confirmed in comparison with 5 other bacteria. Also, during the 120-days period, the complex was stable. The results suggested that it can be used in real samples (R 2 ¼ .9865). This designed colorimetric immunoassay strategy can be used as an alternative, user-friendly and on-site tool for the rapid detection of Brucella spp. compared to other common methods with high sensitivity and specificity in a short time.

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“…Therefore, its diagnosis plays an important role in the disease assessment because of its mortal rate rounds between 50-60% [168]. Recently, Narmani et al developed an ultrasensitive and selective fluorescence DNA biosensor based on AuNPs and magnetic NPs for the determination of the bacteria's O1 OmpW gene [169].…”

Section: Bacterial Pathogen Detectionmentioning

confidence: 99%

Biosensors for the Detection of Bacterial and Viral Clinical Pathogens

Henríquez

Brenes-Acuña

Castro-Rojas

et al. 2020

Sensors

103

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Biosensors are measurement devices that can sense several biomolecules, and are widely used for the detection of relevant clinical pathogens such as bacteria and viruses, showing outstanding results. Because of the latent existing risk of facing another pandemic like the one we are living through due to COVID-19, researchers are constantly looking forward to developing new technologies for diagnosis and treatment of infections caused by different bacteria and viruses. Regarding that, nanotechnology has improved biosensors’ design and performance through the development of materials and nanoparticles that enhance their affinity, selectivity, and efficacy in detecting these pathogens, such as employing nanoparticles, graphene quantum dots, and electrospun nanofibers. Therefore, this work aims to present a comprehensive review that exposes how biosensors work in terms of bacterial and viral detection, and the nanotechnological features that are contributing to achieving a faster yet still efficient COVID-19 diagnosis at the point-of-care.

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Highly sensitive and accurate detection of Vibrio cholera O1 OmpW gene by fluorescence DNA biosensor based on gold and magnetic nanoparticles

Cited by 61 publications

References 27 publications

Folic acid functionalized nanoparticles as pharmaceutical carriers in drug delivery systems

Folic acid functionalized nanoparticles as pharmaceutical carriers in drug delivery systems

Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Biosensors for the Detection of Bacterial and Viral Clinical Pathogens

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