Designing an immunosensor for detection of
            <i>Brucella abortus</i>
            based on coloured silica nanoparticles

Shams, Arash; Zarif, Bahareh Rahimian

doi:10.1080/21691401.2019.1626403

Cited by 10 publications

(8 citation statements)

References 31 publications

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“…As a new nanomaterial, SiNPs have significant advantages, namely, stability and feasibility, compared with other nanoparticles. Based on their stability, bioavailability, colorfulness, sensitivity, and specificity, SiNPs have shown great promise in immunology ( 27 ), virus detection ( 28 ), cell biology ( 29 ), and environmental monitoring ( 30 ). Blue SiNPs as a new nanomaterial were used as a signal probe and SPA was used as a capture probe to catch and immobilize antibodies in the serum as well as combined with the secondary antibody on the C line in our research.…”

Section: Discussionmentioning

confidence: 99%

Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles

Wang

Lian

et al. 2021

Front. Vet. Sci.

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Brucellosis is a highly contagious zoonosis chronic infectious disease with a strong latent capability to endanger human health and economic development via direct or indirect ways. However, the existing methods for brucellosis diagnosis are time-consuming and expensive as they require a tedious experimental procedure and a sophisticated experimental device and performance. To overcome these defects, it is truly necessary to establish a real-time, on-site, and rapid detection method for human brucellosis. Here, a lateral flow immunoassay (LFIA) with a rapid, sensitive, and alternative diagnostic procedure for human brucellosis with a high degree of accuracy was developed based on blue silica nanoparticles (SiNPs), Staphylococcal protein A (SPA), and surface Lipopolysaccharide of Brucella spp. (LPS), which can be applied for rapid and feasible detection of human brucellosis. To our knowledge, this is the first report that uses blue SiNPs as a signal probe of LFIA for the rapid diagnosis of human brucellosis. The precursor of blue SiNPs@SPA such as colorless SiNPs and blue SiNPs was synthesized at first and then coupled with SPA onto the surface of blue SiNPs by covalent bond to prepare blue SiNPs@SPA as a capture signal to catch the antibody in the brucellosis-positive serum. When SPA was combined with the antibodies in the brucellosis-positive serum, it was captured by LPS on the test line, forming an antigen–antibody sandwich structure, resulting in the T line turning blue. Finally, the results showed that it is acceptable to use blue SiNPs as visible labels of LFIA, and standard brucellosis serum (containing Brucella spp. antibody at 1,000 IU/ml) could be detected at a dilution of 10−5 and the detection limit of this method was 0.01 IU/ml. Moreover, it also demonstrated good specificity and accuracy for the detection of real human serum samples. Above all, the blue SiNPs-based LFIA that we developed provides a rapid, highly accurate, and inexpensive on-site diagnosis of human brucellosis, and shows great promise in clinical diagnostics for other diseases.

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Section: Discussionmentioning

confidence: 99%

Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles

Wang

Lian

et al. 2021

Front. Vet. Sci.

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“…The intensity of the color change correlated with the concentration of bacterial cells and measurement of the absorbance of C.I. Reactive Blue 21 at 670 nm could quantitatively indicate the number of B. abortus cells (Shams et al, 2019).…”

Section: Detection Of Bacterial Pathogens Using Biosensing Techniquesmentioning

confidence: 99%

“…Of all nanomaterials, GNPs have all the features needed for electrochemical and optical bioassay including nontoxic, simple and fast synthesis, good performance, high surface area‐to‐volume ratio, and high electrical conductivity (Mittal et al, 2017). Besides, MNPs are also used for the specific characterization of pathogenic bacteria due to their remarkable enzyme activity, low cost, ease of synthesis, and possibility of quick separation by a magnet (Shams et al, 2019).…”

Section: Nanomaterial‐based Biosensorsmentioning

confidence: 99%

“…countries. The disease is extremely important from both aspects of human health risks and the livestock economy(Shams et al, 2019). Several species of the Brucella genus are involved in the development of brucellosis; of which, four classical species B. melitensis, Brucella abortus, Brucella suis, and Brucella canis are considered as the main causative agents of human infections(Pal et al, 2017).…”

mentioning

confidence: 99%

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Advanced nano biosensors for rapid detection of zoonotic bacteria

Ahangari

Mahmoodi

Mohammadzadeh

2022

Biotech & Bioengineering

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An infectious disease that is transmitted from animals to humans and vice-versa is called zoonosis. Bacterial zoonotic diseases can re-emerge after they have been eradicated or controlled and are among the world's major health problems which inflict tremendous burden on healthcare systems. The first step to encounter such illnesses can be early and precise detection of bacterial pathogens to further prevent the following losses due to their infections. Although conventional methods for diagnosing pathogens, including culture-based, polymerase chain reaction-based, and immunological-based techniques, benefit from their advantages, they also have their own drawbacks, for example, taking long time to provide results, and requiring laborious work, expensive materials, and special equipment in certain conditions.Consequently, there is a greater tendency to introduce simple, innovative, quicker, accurate, and low-cost detection methods to effectively characterize the causative agents of infectious diseases. Biosensors, therefore, seem to practically be one of those novel promising diagnostic tools on this aim. These are effective and reliable elements with high sensitivity and specificity, that their usability can even be improved in medical diagnostic systems when empowered by nanoparticles. In the present review, recent advances in the development of several bio and nano biosensors, for rapid detection of zoonotic bacteria, have been discussed in details.

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“…Sandwich structure of PMNPs B. abortus -blue-SiNPs was then formed and separated by a magnet. Using a spectrophotometer, the absorbance of the blue color released from the silica structure was measured alongside the visible color shift to assess the presence of the bacterial cells in the samples (Shams, Rahimian Zarif, Salouti, Shapouri, & Mirzaii, 2019).…”

Section: Sandwich Immunoassay Based On the Silica-and Magnetic-nanoparticles For Detection Of Brucellamentioning

confidence: 99%

Biosensors functionalized with nanoparticles for rapid detection of Brucella

Ahangari

Mahmoodi

Mohammadzadeh

2021

Preprint

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Brucellosis is an infectious bacterial disease that mainly affects ruminants and humans and its detection is still a significant challenge in particular, in the developing countries. At present, methods used to diagnose brucellosis consist of microbiological, biochemical, immunological, and molecular techniques which may be time-consuming, laborious, expensive, and dependent on special equipment, while they may not be suitable for on-site miniaturized detection. Thus, development of novel methods that can characterize Brucella spp with minimal equipment is invaluable for rapid and easy detection of these pathogenic bacteria especially in basic laboratories or even in the farm fields. The nanotechnology-driven approach can be successfully applied using different nanoparticles for the diagnosis of pathogens. Nanobiosensors are therefore, one of those promising solutions in this case. These sensors are efficient tools with diverse biological applications which can identify pathogenic bacteria with relatively high sensitivity and specificity. In this context, new nanobiosensors, developed based on various nanoparticles, for rapid detection of Brucella spp have been discussed.

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Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Cited by 10 publications

References 31 publications

Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles

Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles

Advanced nano biosensors for rapid detection of zoonotic bacteria

Biosensors functionalized with nanoparticles for rapid detection of Brucella

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