Highly sensitive amperometric detection of bilirubin using enzyme and gold nanoparticles on sol–gel film modified electrode

Kannan, Palanisamy; Chen, Hailan; Lee, Victor Tswen-Wen; Kim, Dong Hwan

doi:10.1016/j.talanta.2011.09.034

Cited by 46 publications

(21 citation statements)

References 52 publications

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“…The electroanalytical performances (detection limit, linear range, and sensitivity) of the MWCNT-SPE and Er-GR-SPE are comparable with earlier reported methods [ 12 , 13 , 15 , 17 , 19 , 24 , 25 , 26 , 27 , 38 , 39 , 40 ], as shown in Table 2 .…”

Section: Discussionsupporting

confidence: 85%

“…Recently, electrochemical sensors have been proven to be suitable alternatives since they are simple and selective. In particular, bilirubin oxidase (BOx) immobilized electrodes have been studied for the selective determination of BR [ 12 , 13 , 14 , 15 , 16 ]. In the presence of molecular oxygen, BOx oxidizes the BR into biliverdin and the concentration of BR is determined indirectly by measuring the depletion of molecular oxygen [ 17 ] or the generation of hydrogen peroxide [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Sensor for Bilirubin Detection Using Screen Printed Electrodes Functionalized with Carbon Nanotubes and Graphene

Thangamuthu

Gabriel

Santschi

et al. 2018

Sensors

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Practice oriented point-of-care diagnostics require easy-to-handle, miniaturized, and low-cost analytical tools. In a novel approach, screen printed carbon electrodes (SPEs), which were functionalized with nanomaterials, are employed for selective measurements of bilirubin, which is an important biomarker for jaundice. Multi-walled carbon nanotubes (MWCNT) and graphene separately deposited on SPEs provide the core of an electrochemical sensor for bilirubin. The electrocatalytic activity towards bilirubin oxidation (bilirubin to biliverdin) was observed at +0.25 V. In addition, a further peak corresponding to the electrochemical conversion of biliverdin into purpurin appeared at +0.48 V. When compared to MWCNT, the graphene type shows a 3-fold lower detection limit (0.3 ± 0.022 nM and 0.1 ± 0.018 nM, respectively), moreover, the graphene type exhibits a larger linear range (0.1–600 µM) than MWCNT (0.5–500 µM) with a two-fold better sensitivity, i.e., 30 nA µM−1 cm−2, and 15 nA µM−1 cm−2, respectively. The viability is validated through measurements of bilirubin in blood serum samples and the selectivity is ensured by inhibiting common interfering biological substrates using an ionic nafion membrane. The presented approach enables the design and implementation of low cost and miniaturized electrochemical sensors.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensor for Bilirubin Detection Using Screen Printed Electrodes Functionalized with Carbon Nanotubes and Graphene

Thangamuthu

Gabriel

Santschi

et al. 2018

Sensors

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“…1 ). BOD is a multi-copper oxidase that has been used in several biotechnological applications, such as, biosensors [ 29 , 30 ], biofuel cells [ 31 – 33 ], and other environmental and industrial applications [ 34 , 35 ]. Due to the wide range of biotechnological applications, BOD was selected as our model enzyme.…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide as a protein matrix: influence on protein biophysical properties

et al. 2015

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BackgroundThis study provides fundamental information on the influence of graphene oxide (GO) nanosheets and glycans on protein catalytic activity, dynamics, and thermal stability. We provide evidence of protein stabilization by glycans and how this strategy could be implemented when GO nanosheets is used as protein immobilization matrix. A series of bioconjugates was constructed using two different strategies: adsorbing or covalently attaching native and glycosylated bilirubin oxidase (BOD) to GO.ResultsBioconjugate formation was followed by FT-IR, zeta-potential, and X-ray photoelectron spectroscopy measurements. Enzyme kinetic parameters (km and kcat) revealed that the substrate binding affinity was not affected by glycosylation and immobilization on GO, but the rate of enzyme catalysis was reduced. Structural analysis by circular dichroism showed that glycosylation did not affect the tertiary or the secondary structure of BOD. However, GO produced slight changes in the secondary structure. To shed light into the biophysical consequence of protein glycosylation and protein immobilization on GO nanosheets, we studied structural protein dynamical changes by FT-IR H/D exchange and thermal inactivation.ConclusionsIt was found that glycosylation caused a reduction in structural dynamics that resulted in an increase in thermostability and a decrease in the catalytic activity for both, glycoconjugate and immobilized enzyme. These results establish the usefulness of chemical glycosylation to modulate protein structural dynamics and stability to develop a more stable GO-protein matrix.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-015-0134-0) contains supplementary material, which is available to authorized users.

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“…The use of a biocathode opens interesting prespectives, as the biocathode itself could act as the biosensing element. The possibility of using cathodic enzymes for biosensing widens the range of possible biomarkers that can be detected such as bilirubin, opening new and exciting biosensing applications [ 34 , 35 ]. Several biocathodes have already been designed to detect toxicants in water, such as arsenic [ 36 ] and mercury [ 37 ].…”

Section: Enzymatic Fuel Cells For Biosensing Applicationsmentioning

confidence: 99%

Enzymatic Fuel Cells: Towards Self-Powered Implantable and Wearable Diagnostics

2018

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With the rapid progress in nanotechnology and microengineering, point-of-care and personalised healthcare, based on wearable and implantable diagnostics, is becoming a reality. Enzymatic fuel cells (EFCs) hold great potential as a sustainable means to power such devices by using physiological fluids as the fuel. This review summarises the fundamental operation of EFCs and discusses the most recent advances for their use as implantable and wearable self-powered sensors.

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Highly sensitive amperometric detection of bilirubin using enzyme and gold nanoparticles on sol–gel film modified electrode

Cited by 46 publications

References 52 publications

Electrochemical Sensor for Bilirubin Detection Using Screen Printed Electrodes Functionalized with Carbon Nanotubes and Graphene

Electrochemical Sensor for Bilirubin Detection Using Screen Printed Electrodes Functionalized with Carbon Nanotubes and Graphene

Graphene oxide as a protein matrix: influence on protein biophysical properties

Enzymatic Fuel Cells: Towards Self-Powered Implantable and Wearable Diagnostics

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