Highly sensitive amperometric detection of cardiac troponin I using sandwich aptamers and screen-printed carbon electrodes

Jo, Hang-Hyun; Her, Jin; Lee, Hee-Hyun; Shim, Yoon‐Bo; Ban, Changill

doi:10.1016/j.talanta.2016.12.091

Cited by 104 publications

(40 citation statements)

References 33 publications

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“…Nowadays, application of screen-printed electrodes (SPEs) for analyte analysis is in the main research focus in electrochemical sensors area, what is due to their reliability, mass production, reproducibility, and low cost [39]. As ductile devices, SPEs can be drawn in different shapes and made from various materials [40]. This study will focus on the design and fabrication of disposable SPE modified with La2O3/Co3O4 nanocomposite for electrochemical detection of carvacrol.…”

Section: Introductionmentioning

confidence: 99%

Carvacrol electrochemical reaction characteristics on screen printed electrode modified with La2O3/Co3O4 nanocomposite

Mohammadi

Beitollahi

Rohani

et al. 2019

J. Electrochem. Sci. Eng.

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Electrochemical characteristics of carvacrol were investigated on a screen-printed electrode (SPE) modified with La2O3/Co3O4 nanocomposite by using voltammetric techniques, which displayed a well-defined peak for sensitive carvacrol determination in phosphate buffer solution (PBS) at pH 7.0. La2O3/Co3O4 nanoparticles demonstrated suitable catalytic activity for carvacrol determination by differential pulse voltammetry (DPV) technique. Besides, determination of carvacrol in a real samples was recognized in the light of electrochemical findings and a validated voltammetric technique for quantitative analysis of carvacrol in a real formulation was proposed. The DPV peak currents were found to be linear in the concentration range of 10.0 to 800.0 μM. The limit of detection (LOD) was found to be 1.0 μM.

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Section: Introductionmentioning

confidence: 99%

Carvacrol electrochemical reaction characteristics on screen printed electrode modified with La2O3/Co3O4 nanocomposite

Mohammadi

Beitollahi

Rohani

et al. 2019

J. Electrochem. Sci. Eng.

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“…The detection limit was 0.21 pM (5.00 × 10 À 6 μg/ml), which is much lower than the clinical cut off level of 400 pg/mL. [4,28] So this is a sufficient sensitive aptasensor to utilize as cTnI quantification.…”

Section: Analytical Performance Of the Aptasensor For Quantification mentioning

confidence: 90%

Predicting the Cardiac Troponin I (cTnl) Aptamer/Methylene Blue Configuration Using Computational Modeling Studies: A Screening Search Method for Constructing Aptasensors

et al. 2020

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Acute Myocardial Infarction (AMI) is the most common cause of mortality, and cardiac troponin I (cTnI) is the gold standard for AMI diagnosis. Some aptamers have been reported to detect cTnI, but there is rarely any structural information on their binding with cTnI. There are many studies involving electrochemical aptasensors with methylene blue (MB) as a redox indicator that does not provide any significant new information. Our study, however, involves the computational modeling of the complexes of a documented 40‐mer ssDNA‐cTnI aptamer with its target and MB. Here, MB was used as a model system to investigate the rather complex interaction modes between small redox molecules and a cTnI‐aptamer. Understanding these modes is an important facet in designing electrochemical aptasensors to ascertain the effectiveness of a redox indicator. In our study, docking and molecular dynamics (MD) were used to provide information on the stability, fluctuations, and interaction analysis of the complexes. Based on computational evaluations, we understand how the structure of the cTnI‐aptamer steers the MB releasing after inducing cTnI. In order to evaluate the sensing performance of the cTnI‐aptamer in the presence of MB experimentally, after covalent immobilization of the 5‐amino terminated cTnI‐aptamer at a pre‐functionalized carboxylated multi‐walled carbon nanotube (COOH‐MWCNT) glassy carbon electrode, some techniques including spectroscopy and voltammetry were employed.

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“…Therefore, it is possible to create aptamer-target-antibody hybrids. Such hybrid-systems were successfully demonstrated to detect cardiovascular biomarkers, such as troponin, but were not yet tested with small molecules [ 26 , 27 ]. The small molecule progesterone could also be detected in a sandwich-like lateral flow assay with a final LOD of 5 nM using gold-immobilized aptamers [ 28 ].…”

Section: Aptasensorsmentioning

confidence: 99%

Aptasensors for Point-of-Care Detection of Small Molecules

et al. 2020

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Aptamers, a group of nucleic acids which can specifically bind to a target molecule, have drawn extensive interest over the past few decades. For analytics, aptamers represent a viable alternative to gold-standard antibodies due to their oligonucleic nature combined with advantageous properties, including higher stability in harsh environments and longer shelf-life. Indeed, over the last decade, aptamers have been used in numerous bioanalytical assays and in various point-of-care testing (POCT) platforms. The latter allows for rapid on-site testing and can be performed outside a laboratory by unskilled labor. Aptamer technology for POCT is not limited just to medical diagnostics; it can be used for a range of applications, including environmental monitoring and quality control. In this review, we critically examine the use of aptamers in POCT with an emphasis on their advantages and limitations. We also examine the recent success of aptasensor technology and how these findings pave the way for the analysis of small molecules in POCT and other health-related applications. Finally, the current major limitations of aptamers are discussed, and possible approaches for overcoming these challenges are presented.

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Highly sensitive amperometric detection of cardiac troponin I using sandwich aptamers and screen-printed carbon electrodes

Cited by 104 publications

References 33 publications

Carvacrol electrochemical reaction characteristics on screen printed electrode modified with La2O3/Co3O4 nanocomposite

Carvacrol electrochemical reaction characteristics on screen printed electrode modified with La2O3/Co3O4 nanocomposite

Predicting the Cardiac Troponin I (cTnl) Aptamer/Methylene Blue Configuration Using Computational Modeling Studies: A Screening Search Method for Constructing Aptasensors

Aptasensors for Point-of-Care Detection of Small Molecules

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