Highly selective fluorescent peptide–based chemosensors for aluminium ions in aqueous solution

Ramezanpour, Sorour; Barzinmehr, Hamed; Shiri, Pezhman; Meier, Chris; Ayatollahi, Seyed Abdulmajid; Mehrazar, Mehrdad

doi:10.1007/s00216-021-03339-y

Cited by 10 publications

(3 citation statements)

References 66 publications

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“…Following the same line, Ramezanpour and coworkers improved the detection ability by replacing the dansyl probe with more water-soluble and cheaper 2-aminobenzoyl moiety, keeping the tripeptide the same to give 15. 67 The authors designed another chemosensor 16 with the same fluorophore based on the heptapeptide sequence Ala–Glu–Pro–Glu–Ala–Glu–Pro, a naturally occurring Apidaecin 1 gene peptide with three glutamic acids, which can potentially act as ligating sites for Al 3+ . Both turn-on fluorescent sensors are highly specific and selective to Al 3+ in water with emission maxima at 475 nm after complexation ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Water-soluble optical sensors: keys to detect aluminium in biological environment

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Water-soluble optical sensors: keys to detect aluminium in biological environment

et al. 2022

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“…Thus, microbially produced nanowires might be designed for effective sensing of a diversity of gases of biomedical, environmental, or practical importance. It may also be possible to tailor protein nanowires for sensing non-volatiles such as proteins (Vanova et al 2021), viruses (Fu et al 2020a), pathogenic bacteria (Bruce and Clapper 2020; Pardoux et al 2019), and metallic ions (Liu et al 2015; Ramezanpour et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Microbial nanowires with genetically modified peptide ligands to sustainably fabricate electronic sensing devices

Lekbach¹,

Ueki²,

Liu³

et al. 2022

Preprint

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Nanowires have substantial potential as the sensor component in electronic sensing devices. However, surface functionalization of traditional nanowire and nanotube materials with short peptides that increase sensor selectivity and sensitivity requires complex chemistries with toxic reagents. In contrast, microorganisms can assemble pilin monomers into protein nanowires with intrinsic conductivity from renewable feedstocks, yielding an electronic material that is robust and stable in applications, but also biodegradable. Here we report that the sensitivity and selectivity of protein nanowire-based sensors can be modified with a simple plug and play genetic approach in which a short peptide sequence, designed to bind the analyte of interest, is incorporated into the pilin protein that is microbially assembled into nanowires. We employed a scalable Escherichia coli chassis to fabricate protein nanowires that displayed either a peptide previously demonstrated to effectively bind ammonia, or a peptide known to bind acetic acid. Sensors comprised of thin films of the nanowires amended with the ammonia-specific peptide had a ca. 100-fold greater response to ammonia than sensors made with unmodified protein nanowires. Protein nanowires with the peptide that binds acetic acid yielded a 4-fold higher response than nanowires without the peptide. The results demonstrate that protein nanowires with enhanced sensor response for analytes of interest can be fabricated with a flexible genetic strategy that sustainably eliminates the energy, environmental, and health concerns associated with other common nanomaterials.

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“…Furthermore, Huang et al reported that a hexameric peptide dimerized through disulfide binding containing two tetraphenylethylenes selectively detected Pb 2+ by fluorescence emission through 1:1 binding (K d = 2.1 × 10 −6 M) in 20% aqueous acetonitrile [8]. Ramezanpour et al reported that trimeric Ser-Glu-Glu and heptameric Ala-Glu-Pro-Glu-Ala-Glu-Pro modified with a 2-aminobenzoyl group selectively detected Al 3+ by fluorescence emission through 1:1 binding (K d = 7.7 × 10 −5 and 5.7 × 10 −5 M, respectively) in aqueous solution [9]. Li et al reported that a pentameric peptide, Glu-Cys-Glu-Glu-Trp, modified with a dansyl group (Dns), selectively detected Ag + by ratiometric and turn-on fluorescence emission through 1:1 binding (K d = 6.4 × 10 −9 M) in aqueous solution [10].…”

Section: Introductionmentioning

confidence: 99%

Pyrene-Modified Cyclic Peptides Detect Cu2+ Ions by Fluorescence in Water

Maekawa,

Sakura,

Furutani

et al. 2024

Processes

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The detection of metal ions is an option for maintaining water quality and diagnosing metal ion-related diseases. In this study, we successfully detected metal ions using fluorescent peptides in water. First, we prepared seven linear (L1–L7) and seven cyclic (C1–C7) peptides containing two pyrenyl (Pyr) units and assessed the response to various metal ions by fluorescence. The results indicated that C1, which contains a hexameric cyclic peptide moiety consisting of Pyr and Gly units, did not show a fluorescent response to metal ions, while the linear L1 corresponding to C1 showed a response to Cu2+, but its selectivity was found to be poor through a competition assay for each metal ion. We then assessed C2–C7 and L2–L7, in which Gly was replaced by His units at various positions in the same manner. The results showed that C2–C7 responded to Cu2+ in a manner dependent on the His position. Additionally, superior selectivity was observed in C7 through a competition assay. These results demonstrate that the structural restriction of peptides and the sequence affect the selective detection of Cu2+ and reveal that peptides with an appropriate structure can accomplish the fluorescent detection of Cu2+ specifically.

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Highly selective fluorescent peptide–based chemosensors for aluminium ions in aqueous solution

Cited by 10 publications

References 66 publications

Water-soluble optical sensors: keys to detect aluminium in biological environment

Water-soluble optical sensors: keys to detect aluminium in biological environment

Microbial nanowires with genetically modified peptide ligands to sustainably fabricate electronic sensing devices

Pyrene-Modified Cyclic Peptides Detect Cu2+ Ions by Fluorescence in Water

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