Highly resolved dose–response functions for drug-modulated bacteria cultivation obtained by fluorometric and photometric flow-through sensing in microsegmented flow

Funfak, Anette; Hartung, Regina; Cao, Jialan; Martin, Karin; Wiesmüller, Karl‐Heinz; Wolfbeis, Otto S.; Köhler, J. Michael

doi:10.1016/j.snb.2009.07.017

Cited by 31 publications

(21 citation statements)

References 32 publications

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“…Life Sci. 2015, 00, 1-12 www.biotecvisions.com salts [64,82,83,88] as well as antibiotics [53,67,68,89,90] and other drugs [84,[91][92][93], and food components [80]. The obtained highly resolved dose/response functions from microfluidic investigations reflect roughly the same EC 50 values as observed in conventional screenings.…”

Section: In Situ Sensing Of Biological Activity and Single-cell Studimentioning

confidence: 53%

“…The spectrum of the analyzed organisms spans from bacteria as E. coli [53,57,67,74,[79][80][81] and Streptomyces [82], to eukaryotic microorganisms as Chlorella vulgaris [83], to multicellular organisms [64,65,84], animal cell lines [85], and human cells cultures such as human embryonic kidney (HEK) [86] and Jurkat cells [86,87]. The test substances involve heavy metal www.els-journal.com Eng.…”

Section: In Situ Sensing Of Biological Activity and Single-cell Studimentioning

confidence: 99%

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Droplet‐based microfluidics for microtoxicological studies

Cao

Köhler

2015

Engineering in Life Sciences

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The specific sensitivity of different organisms, cells, and tissues against chemicals, the huge number of produced and applied substances, and the combinatorial explosion of effects by the combination of substances demand new concepts for the efficient determination of toxicological dose/response relations. The generation, processing, and characterization of microdroplets of different chemical compositions enable microfluidic approaches to offer a very promising strategy for strongly miniaturized toxicological screenings. Over the last decade, several developments demonstrated the applicability of droplet-based microfluidic systems for toxicological studies. We review the current state of developments in microfluidics for toxicological applications. These have become powerful alternatives to microtiter plates and are very promising for replacing the standard methods in many fields. This article will focus on toxicological dose/response screenings with the variation of droplet composition, particularly on the possibility of generating "concentration spaces," as well as on the applied sensing principles of organisms and cells inside the microdroplets.

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Section: In Situ Sensing Of Biological Activity and Single-cell Studimentioning

confidence: 53%

Section: In Situ Sensing Of Biological Activity and Single-cell Studimentioning

confidence: 99%

Droplet‐based microfluidics for microtoxicological studies

Cao

Köhler

2015

Engineering in Life Sciences

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“…Following earlier cultivation experiments with E. coli bacteria in microfluid segment [34], fluid segments of 400-450 nl were generated and inoculated with about 100 cells per segment. A strong growth was evaluated after 23 h by measuring the autofluorescence intensity at 470 nm and light scattering at 505 nm.…”

Section: Evaluation Of Dose-related Effects Of Individual Substancementioning

confidence: 99%

Investigation of mixture toxicity of widely used drugs caffeine and ampicillin in the presence of an ACE inhibitor on bacterial growth using droplet-based microfluidic technique

Cao

Goldhan

Martin

et al. 2013

Green Processing and Synthesis

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Droplet-based microfluidic technique is very suitable for a variety of screening processes. The cultivation within nanoliter segments and multidimensional microtoxicological screening of the Gram-negative bacterium Escherichia coli were studied under droplet-based microfluidic conditions. In order to evaluate the toxicity of the binary and ternary mixtures of antibiotic ampicillin and caffeine in the presence of the angiotensin-converting enzyme inhibitor captopril, a time-resolved optical double endpoint detection unit was applied. It included a microflow-through fluorimeter and photometer, which can simultaneously analyze changes in the endogenous autofluorescence signal and the cell density of E. coli cultivated inside 450-nl microfluid segments. As a result, strong nonlinear combination effects and a concentration-dependent antagonistic effect, as well as formation of activity summits on bolographic maps, were found. Our findings confirm the importance of multiparameter investigations for toxicological studies and could be taken into account in medical practice.

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“…In their approach, sequences of microfluid segments behave as artificial compartments for complete separation of small reaction or cultivation volumes for a large spectrum of different targets for toxicological studies. In this work the authors present the simultaneous measurement of the metabolic activity readout by the fluorescence signals of pH-dependent microbeads and the microorganism as well as the growth of the E. coli culture by scattering measurement inside micro fluid segments [96]. …”

Section: Bioanalytical Analysis Technologiesmentioning

confidence: 99%

Particles and microfluidics merged: perspectives of highly sensitive diagnostic detection

et al. 2011

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There is a growing need for diagnostic technologies that provide laboratories with solutions that improve quality, enhance laboratory system productivity, and provide accurate detection of a broad range of infectious diseases and cancers. Recent advances in micro- and nanoscience and engineering, in particular in the areas of particles and microfluidic technologies, have advanced the “lab-on-a-chip” concept towards the development of a new generation of point-of-care diagnostic devices that could significantly enhance test sensitivity and speed. In this review, we will discuss many of the recent advances in microfluidics and particle technologies with an eye towards merging these two technologies for application in medical diagnostics. Although the potential diagnostic applications are virtually unlimited, the most important applications are foreseen in the areas of biomarker research, cancer diagnosis, and detection of infectious microorganisms.

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Highly resolved dose–response functions for drug-modulated bacteria cultivation obtained by fluorometric and photometric flow-through sensing in microsegmented flow

Cited by 31 publications

References 32 publications

Droplet‐based microfluidics for microtoxicological studies

Droplet‐based microfluidics for microtoxicological studies

Investigation of mixture toxicity of widely used drugs caffeine and ampicillin in the presence of an ACE inhibitor on bacterial growth using droplet-based microfluidic technique

Particles and microfluidics merged: perspectives of highly sensitive diagnostic detection

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