Highly polymorphic microsatellite loci in the rice‐ and maize‐infecting fungal pathogen Rhizoctonia solani anastomosis group 1 IA

Abstract

Ten polymorphic microsatellite loci were isolated and characterized from the rice- and maize-infecting Basidiomycete fungus Rhizoctonia solani anastomosis group AG-1 IA. All loci were polymorphic in two populations from Louisiana in USA and Venezuela. The total number of alleles per locus ranged from four to eight. All 10 loci were also useful for genotyping soybean-infecting R. solani AG-1 isolates from Brazil and USA. One locus, TC06, amplified across two other AG groups representing different species, showing species-specific repeat length polymorphism. This marker suite will be used to determine the global population structure of this important pathogenic fungus.

Divergence Between Sympatric Rice- and Maize-Infecting Populations of Rhizoctonia solani AG-1 IA from Latin America

“…Ten polymorphic co-dominant microsatellite simple sequence repeat (SSR) loci were used to genotype the isolates using fluorescentlabeled primers as reported by Zala et al (72). Two control isolates (Tc05US_KatC6 and Tc05VNZ_A1A1) were included in every run of 94 samples.…”

“…An additional test for reproducibility of the microsatellite allele assignments was made by comparing sizes of each allele at every locus for the maize population from Santa Rosalía, which was independently genotyped twice. The data from this second independent genotyping were used to describe the microsatellite genotyping system (72). The statistical binning of alleles into fragment size categories consistent with the respective unit increment described by Zala et al (72) was implemented using the program Flexibin (3).…”

“…The data from this second independent genotyping were used to describe the microsatellite genotyping system (72). The statistical binning of alleles into fragment size categories consistent with the respective unit increment described by Zala et al (72) was implemented using the program Flexibin (3). Based on the observed number of repeats found for the microsatellite loci in this and three other studies (6,7,15), we assumed a stepwise mutation model (SMM) in all of the analyses.…”

Assessing genetic diversity in the web blight pathogen Thanatephorus cucumeris (anamorph = Rhizoctonia solani) subgroups AG-1-IE and AG-1-IF with molecular markers

“…Isolates were tested with 10 markers that have been reported to be polymorphic and reliable in characterizing genetic diversity in R. solani Zala et al 2008). Only four molecular markers, URP: URP2R (5′-CCCAGCAACTGATCGCACAC-3′) and URP6R (5′-GCAAGCTGGTGGGAGGTAC-3′) , and ISSR: ISSR10 (5′-CACCACCACCAC-CAC-3′) ) and (GACA) 4 (Ureña-Padilla et al 2002), were informative for assessing diversity.…”

Molecular markers for genotyping anastomosis groups and understanding the population biology of Rhizoctonia species.

“…McDonald, 1997) ．さらに近年では，Rhizoctonia 属の特定の 種内系統群あるいは分類群を対象とした，集団遺伝学解析用 の分子マーカーが作成されつつある (Chaijuckam et al, 2010;Ferrucho et al, 2009;Zala et al, 2008 (Naito and Sugimoto, 1978;Naito et al, 1995;Priyatmojo et al, 2001 , 1997;Guo et al, 2003;Guo et al, 2006;Inagaki, 1998;Inagaki et al, 2004;Ogoshi and Ui, 1983 and Inagaki, 2014;Godoy-Lutz et al, 2008;Johanson et al, 1998;Justesen et al, 2003;Lees et al, 2002;Matsumoto, 2002;Okubara et al, 2008;Sayler and Yang, 2007;Toda et al, 2004 …”