Highly polarized HLA class II antigen processing and presentation by human intestinal epithelial cells.

Hershberg, Robert M.; Cho, D H; Youakim, Adel; Bradley, Mikaela; Lee, J S; Framson, Paul E.; Nepom, Gerald T.

doi:10.1172/jci3201

Cited by 170 publications

(111 citation statements)

References 42 publications

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“…We found that presentation of Ags was significantly greater at the basolateral surface than at the apical surface. These findings extend the important observations of Hershberg et al (21) who demonstrated preferential basolateral presentation with a human IEC line that expressed HLA-DR. To the best of our knowledge, our findings represent the first demonstration of Ag processing by primary epithelial cells.…”

Section: Discussionsupporting

confidence: 90%

“…In contrast, Hershberg et al (21) reported that polarized IEC process as well as present Ags to HLA-DR-restricted T cells. One explanation for this difference may be that because epithelial cells from the reproductive tract are under hormonal control, the presence of estradiol and/or progesterone may be essential for processing to occur.…”

Section: Discussionmentioning

confidence: 91%

“…Subsequent studies with other Ags confirmed these results, although most Ags were inefficiently presented (19). Hershberg et al (20,21) used a polarized IEC line transfected to express HLA-DR to demonstrate class II-tetanus toxoid Ag presentation to restricted CD4 ϩ T cells. These studies demonstrated that epithelial cells preferentially presented Ag at the basolateral surface (21).…”

Section: Polarized Uterine Epithelial Cells Preferentially Presentmentioning

confidence: 93%

“…Hershberg et al (20,21) used a polarized IEC line transfected to express HLA-DR to demonstrate class II-tetanus toxoid Ag presentation to restricted CD4 ϩ T cells. These studies demonstrated that epithelial cells preferentially presented Ag at the basolateral surface (21). We more recently demonstrated that uterine cell suspensions consisting of human epithelium and stromal cells were capable of presenting Ags to autologous T cells (17).…”

Section: Polarized Uterine Epithelial Cells Preferentially Presentmentioning

confidence: 99%

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Polarized Uterine Epithelial Cells Preferentially Present Antigen at the Basolateral Surface: Role of Stromal Cells in Regulating Class II-Mediated Epithelial Cell Antigen Presentation

Wira

Rossoll

Young³

2005

The Journal of Immunology

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To study Ag presentation in the female reproductive tract, DO11.10 TCR transgenic mice specific for the class II MHC-restricted OVA323–339 peptide and nontransgenic BALB/c mice were used. We report here that freshly isolated uterine epithelial cells, uterine stromal, and vaginal APCs present OVA and OVA323–339peptide to naive- and memory T cells, which is reduced when cells are incubated with Abs to CD80 and 86. To determine whether polarized primary epithelial cells present Ags, uterine epithelial cells were cultured on cell inserts in either the upright or inverted position. After reaching confluence, as indicated by high transepithelial resistance (>2000 ohms/well), Ag presentation by epithelial cells incubated with memory T cells and OVA323–339 peptide placed on the basolateral surface (inverted) was 2- to 3-fold greater than that seen with epithelial cells in contact with T cells and peptide on the apical surface (upright). In contrast, whereas freshly isolated epithelial cells process OVA, polarized epithelial cells did not. When epithelial cells grown upright on inserts were incubated with T cells and OVA323–339 peptide, coculture with either hepatocyte growth factor or conditioned stromal medium increased epithelial cell Ag presentation (∼90% higher than controls). These studies indicate that uterine stromal cells produce a soluble factor(s) in addition to a hepatocyte growth factor, which regulates epithelial cell Ag presentation. Overall, these results demonstrate that polarized epithelial cells are able to present Ags and suggest that uterine stromal cells communicate with epithelial cells via a soluble factor(s) to regulate Ag presentation in the uterus.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 91%

Section: Polarized Uterine Epithelial Cells Preferentially Presentmentioning

confidence: 93%

Section: Polarized Uterine Epithelial Cells Preferentially Presentmentioning

confidence: 99%

See 2 more Smart Citations

Polarized Uterine Epithelial Cells Preferentially Present Antigen at the Basolateral Surface: Role of Stromal Cells in Regulating Class II-Mediated Epithelial Cell Antigen Presentation

Wira

Rossoll

Young³

2005

The Journal of Immunology

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“…Mucosal epithelium can serve as a physical barrier to prevent the exposure of potentially harmful antigens to underlying lymphoid tissues and this is primarily achieved through adherens and tight junctions. Gut epithelium also expresses MHC Class II molecules that lack traditional co-stimulatory molecules, thus promoting tolerance after binding to food proteins, rather than reactivity [3]. Peyer's patches contain organized lymphoid follicles consisting of a B-cell germinal center surrounded by T lymphocytes.…”

Section: Introductionmentioning

confidence: 99%